Measured Effects of Surface Cloth Impressions on Polar Backscatter and Comparison with a Reflection Grating Model

Integrated polar backscatter has been shown to have potential applications to composites, especially for the detection of matrix cracking, delaminations, fiber waviness, fiber fracture, inclusions and porosity [1–11]. The method was attractive because it avoided several measurement limitations inherent to conventional pulse echo techniques. Polar backscatter, however, has not been without its disadvantages. It has been reported that surface texture introduces unwanted artifacts in images made using the polar backscatter method [12]. One suggested method to overcome this limitation was the use of stripable coatings, which are paints that approximately match the impedance of the composite surface and have the effect of physically “smoothing” the surface impressions away [13]. After ultrasonic testing, these paints can be removed, but this method entails additional part handling and increases the cost of production

High contrast and resolution 3-D ultrasonography with a clinical linear transducer array scanned in a rotate-translate geometry

International audienceWe propose a novel solution for volumetric ultrasound imaging using single-side access 3-D synthetic-aperture scanning of a clinical linear array. This solution is based on an advanced scanning geometry and a software-based ultrasound platform. The rotate-translate scanning scheme increases the elevation angular aperture by pivoting the array [-45° to 45°] around its array axis (axis along the row of its elements) and then, scans the imaged object for each pivoted angle by translating the array perpendicularly to the rotation axis. A theoretical basis is presented so that the angular and translational scan sampling periods can be best adjusted for any linear transducer array. We experimentally implemented scanning with a 5-MHz array. In vitro characterization was performed with phantoms designed to test resolution and contrast. Spatial resolution assessed based on the full-width half-maximum of images from isolated microspheres was increased by a factor 3 along the translational direction from a simple translation scan of the array. Moreover, the resolution is uniform over a cross-sectional area of 4.5 cm 2. Angular sampling periods were optimized and tapered to decrease the scan duration while maintaining image contrast (contrast at the center of a 5 mm cyst on the order of-26 dB for 4° angular period and a scan duration of 10 s for a 9cm 3 volume). We demonstrate that superior 3-D US imaging can be obtained with a clinical array using our scanning strategy. This technique offers a promising and flexible alternative to development of costly matrix arrays toward the development of sensitive volumetric ultrasonography

Measured Effects of Surface Cloth Impressions on Polar Backscatter and Comparison with a Reflection Grating Model

Integrated polar backscatter has been shown to have potential applications to composites, especially for the detection of matrix cracking, delaminations, fiber waviness, fiber fracture, inclusions and porosity [1–11]. The method was attractive because it avoided several measurement limitations inherent to conventional pulse echo techniques. Polar backscatter, however, has not been without its disadvantages. It has been reported that surface texture introduces unwanted artifacts in images made using the polar backscatter method [12]. One suggested method to overcome this limitation was the use of stripable coatings, which are paints that approximately match the impedance of the composite surface and have the effect of physically “smoothing” the surface impressions away [13]. After ultrasonic testing, these paints can be removed, but this method entails additional part handling and increases the cost of production.</p

Non-Invasive Ultrasonic Description of Tumor Evolution

International audienceSimple SummaryDuring tumor evolution, heterogeneous structural and functional changes occur in the tumor microenvironment. These complex changes have pro- or anti-tumorigenesis effects and have an impact on therapy efficiency. Therefore, the tumor microenvironment needs to be non-invasively characterized over time. The aim of this preclinical work is to compare the sensitivity of modifications occurring during tumor evolution of volume, immunohistochemistry and non-invasive quantitative ultrasound parameters (Shear Wave Elastography and dynamic Contrast-Enhanced Ultrasound) and to study the link between them. The complementary evaluation over time of multiple morphological and functional parameters during tumor growth underlines the need to integrate histological, morphological, functional, and, ultimately, genomic information into models that can consider the temporal and spatial variability of features to better understand tumor evolution.AbstractPurpose: There is a clinical need to better non-invasively characterize the tumor microenvironment in order to reveal evidence of early tumor response to therapy and to better understand therapeutic response. The goals of this work are first to compare the sensitivity to modifications occurring during tumor growth for measurements of tumor volume, immunohistochemistry parameters, and emerging ultrasound parameters (Shear Wave Elastography (SWE) and dynamic Contrast-Enhanced Ultrasound (CEUS)), and secondly, to study the link between the different parameters. Methods: Five different groups of 9 to 10 BALB/c female mice with subcutaneous CT26 tumors were imaged using B-mode morphological imaging, SWE, and CEUS at different dates. Whole-slice immunohistological data stained for the nuclei, T lymphocytes, apoptosis, and vascular endothelium from these tumors were analyzed. Results: Tumor volume and three CEUS parameters (Time to Peak, Wash-In Rate, and Wash-Out Rate) significantly changed over time. The immunohistological parameters, CEUS parameters, and SWE parameters showed intracorrelation. Four immunohistological parameters (the number of T lymphocytes per mm2 and its standard deviation, the percentage area of apoptosis, and the colocalization of apoptosis and vascular endothelium) were correlated with the CEUS parameters (Time to Peak, Wash-In Rate, Wash-Out Rate, and Mean Transit Time). The SWE parameters were not correlated with the CEUS parameters nor with the immunohistological parameters. Conclusions: US imaging can provide additional information on tumoral changes. This could help to better explore the effect of therapies on tumor evolution, by studying the evolution of the parameters over time and by studying their correlations

Quantitative Ultrasound and Immunohistochemistry Characterization at Different Stages of Murine Tumor Development

International audienc

Impact of Recirculation in Dynamic Contrast-Enhanced Ultrasound: A Simulation Study

International audienc

Relative Quality of Tumor Growth Equations Describing Volume and Vascular Evolution Assessed with Ultrasound

International audienc

Volumetric and Simultaneous Photoacoustic and Ultrasound Imaging with a Conventional Linear Array in a Multiview Scanning Scheme

Volumetric dual photacoustic (PA) / ultrasonic (US) imaging with precise spatial and temporal coregistration can provide valuable and complementary information for diagnosis and monitoring. Considerable research has sought to combine 3D PA/US imaging in configurations that can be transferred to clinical application but technical compromises currently result in poor image quality either for photoacoustic or ultrasonic modes. Simultaneous 3D PA/US tomography was implemented here by interlacing PA and US acquisitions during the rotate-translate scan of a 5-MHz linear array (12 angles and 30 mm translational range to image a cylindrical volume of 21 mm diameter and 19 mm length within 21 seconds). Volumetric image reconstruction was performed with synthetic aperture approaches. An original calibration method was developed to estimate 6 geometrical parameters and 1 temporal off-set providing sharpest and best superimposed reconstructions. Calibration thread phantom design and choice of metrics to build the cost function were based on analysis of a numerical phantom and the final selection demonstrates a high estimation accuracy of the 7 parameters. Experimental estimations validated the calibration repeatability. Experiments in an additional phantom showed a superposition distance between thread centers identified in the PA and US images to be smaller than 10% of the acoustic wavelength, and a spatial resolution on the order of the wavelength. Dual mode 3D imaging with high-quality co-registration and excellent, uniform spatial resolution was further demonstrated on phantoms with complementary contrasts, and should contribute to more sensitive and robust imaging to detect and follow biological changes or the accumulation of nanoagents in living systems

VEGFR2-targeted contrast-enhanced ultrasound to distinguish between two antiangiogenic treatments

International audienceThe aim of this study was to evaluate the capacity of BR55, an ultrasound contrast agent specifically targeting vascular endothelial growth factor receptor 2 (VEGFR2), to distinguish the specific anti-VEGFR2 therapy effect of sunitinib from other anti-angiogenic effects of a therapy (imatinib) that does not directly inhibit VEGFR2. Sunitinib, imatinib and placebo were administered daily for 11 d (264 h) to 45 BalbC mice bearing ectopic CT26 murine colorectal carcinomas. During the course of therapy, B-mode ultrasound, contrast-enhanced ultrasound and VEGFR2-targeted contrast-enhanced ultrasound were performed to assess tumor morphology, vascularization and VEGFR2 expression, respectively. The angiogenic effects on these three aspects were characterized using tumor volume, contrast-enhanced area and differential targeted enhancement. Necrosis, microvasculature and expression of VEGFR2 were also determined by histology and immunostaining. B-Mode imaging revealed that tumor growth was significantly decreased in sunitinib-treated mice at day 11 (p < 0.05), whereas imatinib did not affect growth. Functional evaluation revealed that the contrast-enhanced area decreased significantly (p < 0.02) and by similar amounts under both anti-angiogenic treatments by day 8 (192 h): −23% for imatinib and −21% for sunitinib. No significant decrease was observed in the placebo group. Targeted contrast-enhanced imaging revealed lower differential targeted enhancement, that is, lower levels of VEGFR2 expression, in sunitinib-treated mice relative to placebo-treated mice from 24 h (p < 0.05) and relative to both placebo- and imatinib-treated mice from 48 h (p < 0.05). Histologic assessment of tumors after the final imaging indicated that necrotic area was significantly higher for the sunitinib group (21%) than for the placebo (8%, p < 0.001) and imatinib (11%, p < 0.05) groups. VEGFR2-targeted ultrasound was able to sensitively differentiate the anti-VEGFR2 effect from the reduced area of tumor with functional flow produced by both anti-angiogenic agents. BR55 molecular imaging was, thus, able both to detect early therapeutic response to sunitinib in CT26 tumors as soon as 24 h after the beginning of the treatment and to provide early discrimination (48 h) between tumor response during anti-angiogenic therapy targeting VEGFR2 expression and response during anti-angiogenic therapy not directly acting on this receptor