The hosts of z=2z=2 QSOs

We present results of the hosts of four high-redshift ($z \approx 2$) and high luminosity (M_B \lsim -28 mag) QSOs, three radio-quiet one radio-loud, imaged in R and K bands. The extensions to the nuclear unresolved source are most likely due to the hosts galaxies of these QSOs, with luminosities at rest-frame 2300\AA of at least 3-7% of the QSO luminosity, and most likely around 6-18% of the QSO luminosity. Our observations show that, if the extensions we have detected are indeed galaxies, extraordinary big and luminous host galaxies are not only a characteristic of radio-loud objects, but of QSOs as an entire class.Comment: 6 pages, 4 Figures, Latex, uses lamuphys.sty, also available at http://www.mpa-garching.mpg.de/~itziar To appear in the Proceedings of the ESO/IAC Conference on 'Quasar Hosts' ESO Astrophysics Symposia eds. D. Clements and I. Perez-Fourno

The Effect of Variability on the Estimation of Quasar Black Hole Masses

We investigate the time-dependent variations of ultraviolet (UV) black hole mass estimates of quasars in the Sloan Digital Sky Survey (SDSS). From SDSS spectra of 615 high-redshift (1.69 < z < 4.75) quasars with spectra from two epochs, we estimate black hole masses, using a single-epoch technique which employs an additional, automated night-sky-line removal, and relies on UV continuum luminosity and CIV (1549A) emission line dispersion. Mass estimates show variations between epochs at about the 30% level for the sample as a whole. We determine that, for our full sample, measurement error in the line dispersion likely plays a larger role than the inherent variability, in terms of contributing to variations in mass estimates between epochs. However, we use the variations in quasars with r-band spectral signal-to-noise ratio greater than 15 to estimate that the contribution to these variations from inherent variability is roughly 20%. We conclude that these differences in black hole mass estimates between epochs indicate variability is not a large contributer to the current factor of two scatter between mass estimates derived from low- and high-ionization emission lines.Comment: 76 pages, 15 figures, 2 (long) tables; Accepted for publication in ApJ (November 10, 2007

Can Survey-based Scenarios Measure Consumer Values for Improved Food Safety?

Demand and Price Analysis, Food Consumption/Nutrition/Food Safety,

Spectral Variability of Quasars in the Sloan Digital Sky Survey. II: The C IV Line

We examine the variability of the high-ionizaton C IV line in a sample of 105 quasars observed at multiple epochs by the Sloan Digital Sky Survey. We find a strong correlation between the change in the C IV line flux and the change in the line width, but no correlations between the change in flux and changes in line center and skewness. The relation between line flux change and line width change is consistent with a model in which a broad line base varies with greater amplitude than the line core. The objects studied here are more luminous and at higher redshift than those normally studied for variability, ranging in redshift from 1.65 to 4.00 and in absolute r-band magnitude from roughly -24 to -28. Using moment analysis line-fitting techniques, we measure line fluxes, centers, widths and skewnesses for the C IV line at two epochs for each object. The well-known Baldwin Effect is seen for these objects, with a slope beta = -0.22. The sample has a median intrinsic Baldwin Effect slope of beta = -0.85; the C IV lines in these high-luminosity quasars appear to be less responsive to continuum variations than those in lower luminosity AGN. Additionally, we find no evidence for variability of the well known blueshift of the C IV line with respect to the low-ionization Mg II line in the highest flux objects, indicating that this blueshift might be useful as a measure of orientation.Comment: 52 pages, 14 figures, accepted for publication in Ap

Propidium monoazide (PMA) and ethidium bromide monoazide(EMA) improve DNA array and high-throughput sequencing ofporcine reproductive and respiratory syndrome virus identification

Pan-viral DNA array (PVDA) and high-throughput sequencing (HTS) are useful tools to identify novel viruses of emerging diseases. However, both techniques have difficulties to identify viruses in clinical samples because of the host genomic nucleic acid content (hg/cont). Both propidium monoazide (PMA) and ethidium bromide monoazide (EMA) have the capacity to bind free DNA/RNA, but are cell membrane-impermeable. Thus, both are unable to bind protected nucleic acid such as viral genomes within intact virions. However, EMA/PMA modified genetic material cannot be amplified by enzymes. In order to assess the potential of EMA/PMA to lower the presence of amplifiable hg/cont in samples and improve virus detection, serum and lung tissue homogenates were spiked with porcine reproductive and respiratory virus (PRRSV) and were processed with EMA/PMA. In addition, PRRSV RT-qPCR positive clinical samples were also tested. EMA/PMA treatments significantly decreased amplifiable hg/cont and significantly increased the number of PVDA positive probes and their signal intensity compared to untreated spiked lung samples. EMA/PMA treatments also increased the sensitivity of HTS by increasing the number of specific PRRSV reads and the PRRSV percentage of coverage. Interestingly, EMA/PMA treatments significantly increased the sensitivity of PVDA and HTS in two out of three clinical tissue samples. Thus, EMA/PMA treatments offer a new approach to lower the amplifiable hg/cont in clinical samples and increase the success of PVDA and HTS to identify viruses