Dígalo con alimentos : jugar, sentir, hacer pensar

En este trabajo se narrará la experiencia acerca de una actividad desarrollada en el marco de la VIII Jornada de Campo en la Chacra Experimental de la Facultad de Agronomía de Azul en el marco del programa "Azul Saludable".Fil: Adam, Sandra Gabriela . Universidad Nacional del Centro de la Provincia de Buenos Aires.Fil: Brescia, María Marcela. Universidad Nacional del Centro de la Provincia de Buenos Aires.Fil: Fittipaldi, Carolina Beatriz. Universidad Nacional del Centro de la Provincia de Buenos Aires.Fil: Salsamendi, Maité . Universidad Nacional del Centro de la Provincia de Buenos Aires

Th17-Gene Expression Profile in Patients with Chronic Venous Disease and Venous Ulcers: Genetic Modulations and Preliminary Clinical Evidence

Chronic venous disease is a condition globally widespread, resulting in a disabling pathological disorder. The CD4 + Th17+ (Cluster Differentiation 4) lymphocytes represent a regulative factor for innate immunity related to the development of complex diseases. Recently, these mechanisms have been associated with vascular disease. The aim of this work is to validate whether the Th17 response correlates with the development of CVI (Chronic venous insufficiency)and CVLUs (chronic venous limbs ulcers) and whether Th17 markers can be used, both as intrinsic risk factors and diagnostic markers, for disease development. PBL derived from peripheral blood samples of patients and controls were subjected to gene expression analysis for IL23R, IL17, SGK1, TGFβ, RORγ, FOXO1, and RANBP1 by qRT-PCR and immunoblot. A post hoc correlation, the diagnostic performance of the target genes, and multivariable analyses were properly conducted. The main expression markers of the CD4 + Th17+ switch were strongly activated in chronic venous insufficiency and in advanced ulceration. The correlation analysis demonstrated the inter-dependence on Th17’s signature modulation. ROC (Receiver Operating Characteristic) analysis defined, for the examined genes, a clinical value as the potential diagnostic markers. Multi-logistic regression studies showed that Th17 markers behave as empirical risk factors for CVD (chronic venous disease) development. Taken together, the present data provide a new hypothesis for the TH17-dependent pathogenesis of CVD, favoring the possibility for the development of new diagnostic, preventive, and therapeutic approaches

RANBP1, a member of the nuclear-cytoplasmic trafficking-regulator complex, is the terminal-striking point of the SGK1-dependent Th17+ pathological differentiation

The Th17+ arrangement is critical for orchestrating both innate and acquired immune responses. In this context, the serum and glucocorticoid regulated kinase 1 (SGK1) exerts a key role in the governance of IL-23R-dependent Th17+ maturation, through the phosphorylation-dependent control of FOXO1 localization. Our previous work has shown that some of the SGK1-key functions are dependent on RAN-binding protein 1 (RANBP1), a terminal gene in the nuclear transport regulation. Here, we show that RANBP1, similarly to SGK1, is modulated during Th17+ differentiation and that RANBP1 fluctuations mediate the SGK1-dependent effects on Th17+ maturation. RANBP1, as the final effector of the SGK1 pathway, affects FOXO1 transport from the nucleus to the cytoplasm, thus enabling RORγt activation. In this light, RANBP1 represents the missing piece, in an essential and rate-limiting manner, underlying the Th17+ immune asset

COVID-19 Severity in Multiple Sclerosis: Putting Data Into Context

Background and objectives: It is unclear how multiple sclerosis (MS) affects the severity of COVID-19. The aim of this study is to compare COVID-19-related outcomes collected in an Italian cohort of patients with MS with the outcomes expected in the age- and sex-matched Italian population. Methods: Hospitalization, intensive care unit (ICU) admission, and death after COVID-19 diagnosis of 1,362 patients with MS were compared with the age- and sex-matched Italian population in a retrospective observational case-cohort study with population-based control. The observed vs the expected events were compared in the whole MS cohort and in different subgroups (higher risk: Expanded Disability Status Scale [EDSS] score > 3 or at least 1 comorbidity, lower risk: EDSS score ≤ 3 and no comorbidities) by the χ2 test, and the risk excess was quantified by risk ratios (RRs). Results: The risk of severe events was about twice the risk in the age- and sex-matched Italian population: RR = 2.12 for hospitalization (p < 0.001), RR = 2.19 for ICU admission (p < 0.001), and RR = 2.43 for death (p < 0.001). The excess of risk was confined to the higher-risk group (n = 553). In lower-risk patients (n = 809), the rate of events was close to that of the Italian age- and sex-matched population (RR = 1.12 for hospitalization, RR = 1.52 for ICU admission, and RR = 1.19 for death). In the lower-risk group, an increased hospitalization risk was detected in patients on anti-CD20 (RR = 3.03, p = 0.005), whereas a decrease was detected in patients on interferon (0 observed vs 4 expected events, p = 0.04). Discussion: Overall, the MS cohort had a risk of severe events that is twice the risk than the age- and sex-matched Italian population. This excess of risk is mainly explained by the EDSS score and comorbidities, whereas a residual increase of hospitalization risk was observed in patients on anti-CD20 therapies and a decrease in people on interferon

DMTs and Covid-19 severity in MS: a pooled analysis from Italy and France

We evaluated the effect of DMTs on Covid-19 severity in patients with MS, with a pooled-analysis of two large cohorts from Italy and France. The association of baseline characteristics and DMTs with Covid-19 severity was assessed by multivariate ordinal-logistic models and pooled by a fixed-effect meta-analysis. 1066 patients with MS from Italy and 721 from France were included. In the multivariate model, anti-CD20 therapies were significantly associated (OR = 2.05, 95%CI = 1.39–3.02, p < 0.001) with Covid-19 severity, whereas interferon indicated a decreased risk (OR = 0.42, 95%CI = 0.18–0.99, p = 0.047). This pooled-analysis confirms an increased risk of severe Covid-19 in patients on anti-CD20 therapies and supports the protective role of interferon

RANBP1 (RAN Binding Protein 1): The Missing Genetic Piece in Cancer Pathophysiology and Other Complex Diseases

RANBP1 encoded by RANBP1 or HTF9A (Hpall Tiny Fragments Locus 9A), plays regulatory functions of the RAN-network, belonging to the RAS superfamily of small GTPases. Through this function, RANBP1 regulates the RANGAP1 activity and, thus, the fluctuations between GTP-RAN and GDP-RAN. In the light of this, RANBP1 take actions in maintaining the nucleus–cytoplasmic gradient, thus making nuclear import–export functional. RANBP1 has been implicated in the inter-nuclear transport of proteins, nucleic acids and microRNAs, fully contributing to cellular epigenomic signature. Recently, a RANBP1 diriment role in spindle checkpoint formation and nucleation has emerged, thus constituting an essential element in the control of mitotic stability. Over time, RANBP1 has been demonstrated to be variously involved in human cancers both for the role in controlling nuclear transport and RAN activity and for its ability to determine the efficiency of the mitotic process. RANBP1 also appears to be implicated in chemo-hormone and radio-resistance. A key role of this small-GTPases related protein has also been demonstrated in alterations of axonal flow and neuronal plasticity, as well as in viral and bacterial metabolism and in embryological maturation. In conclusion, RANBP1 appears not only to be an interesting factor in several pathological conditions but also a putative target of clinical interest

Antiarrhythmic action of chronic melatonin in female rats

Introduction: Arrhythmias are complications during reperfusion of the ischemic myocardium. Acute melatonin is antiarrhythmic, but there is less information about females´ animals receiving chronic treatment. Objective: We aim to evaluate the electrophysiological effects of melatonin in female rats. Methodology: Wistar Kyoto rats (WKY), ten weeks old, received melatonin dissolved in water (3-5 mg/kg/day, MEL) during 15 days. After this period, we evaluated the electrocardiogram in isolated hearts undergoing a 10 minutes regional ischemia, followed by reperfusion of 10 minutes. All procedures were approved by local Institutional Animal Care and Use Committees (106/2017). Results: Melatonin treatment shortened PR and QRS intervals and prolonged the QT interval and QT corrected for heart rate. Melatonin chronic treatment prevented the incidence of ventricular fibrillation during reperfusion (WKY 6/9; WKY+MEL 1/10; P=0.0198 by Fisher´s exact test). Conclusion: Our results confirmed the cardioprotective effects of oral melatonin administration in female rats and showed electrocardiographic modifications compatible with increased conduction and delayed myocardial repolarization as possible antiarrhythmic mechanisms. Universidad Nacional de Cuyo, SIIP project 06-J505Fil: Prado, Natalia Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Brescia, Carolina Gabriela. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Carrión, Adriana Miriam. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Renna, Nicolas Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; ArgentinaFil: Maitello, Roberto Miguel. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Diez, Emiliano Raúl. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaSociedad Chilena de Ciencias Fisiológicas + Asociación Latinoamericana de Ciencias Fisiológicas 2020 Joint MeetingSantiagoChileSociedad Chilena de Ciencias Fisiológicas y Asociación Latinoamericana de Ciencias Fisiológica

Informe final del proyecto: La formación inicial magisterial en Uruguay y el desarrollo de competencias en investigación educativa, escritura académica y práctica reflexiva, para el ejercicio de la práctica profesional

Esta investigación se propuso describir, analizar e interpretar las percepciones de los agentes involucrados en la formación inicial de los estudiantes de magisterio del Uruguay sobre sus competencias para afrontar la práctica profesional, especialmente las que se vinculan con la investigación educativa, la producción de escritura académica, y la reflexión sobre sus prácticas pedagógicas. En la misma línea, continúa y profundiza las investigaciones sobre la construcción de la identidad profesional del docente de educación primaria, y las características de la práctica preprofesional magisterial, ambas a cargo del mismo equipo y seleccionadas por este Fondo en sus ediciones 2017 y 2018. Desde un enfoque y metodología cualitativa, se realizaron entrevistas semiestructuradas a docentes formadores y maestras noveles, así como grupos de discusión con estudiantes de último año de la carrera. El estudio tuvo alcance nacional e involucró a los centros de formación docente de Florida, Mercedes, Minas, Montevideo, y Salto. Los principales hallazgos se refieren a tres dimensiones de la formación inicial magisterial que se vinculan directamente con el ejercicio de una profesión que se proyecta con estatus universitario: investigación, escritura académica, y práctica reflexiva. Muestran algunas fortalezas, dadas a partir de prácticas de docentes puntuales, y sobre todo la necesidad de impulsar estrategias formativas que habiliten y potencien el desarrollo de competencias en esas tres dimensiones, hoy muy escasas, tanto en estudiantes de último año de la carrera como en egresadas recientes.Agencia Nacional de Investigación e Innovació

Informe final del proyecto: La formación inicial magisterial en Uruguay y el desarrollo de competencias en investigación educativa, escritura académica y práctica reflexiva, para el ejercicio de la práctica profesional

Esta investigación se propuso describir, analizar e interpretar las percepciones de los agentes involucrados en la formación inicial de los estudiantes de magisterio del Uruguay sobre sus competencias para afrontar la práctica profesional, especialmente las que se vinculan con la investigación educativa, la producción de escritura académica, y la reflexión sobre sus prácticas pedagógicas. En la misma línea, continúa y profundiza las investigaciones sobre la construcción de la identidad profesional del docente de educación primaria, y las características de la práctica preprofesional magisterial, ambas a cargo del mismo equipo y seleccionadas por este Fondo en sus ediciones 2017 y 2018. Desde un enfoque y metodología cualitativa, se realizaron entrevistas semiestructuradas a docentes formadores y maestras noveles, así como grupos de discusión con estudiantes de último año de la carrera. El estudio tuvo alcance nacional e involucró a los centros de formación docente de Florida, Mercedes, Minas, Montevideo, y Salto. Los principales hallazgos se refieren a tres dimensiones de la formación inicial magisterial que se vinculan directamente con el ejercicio de una profesión que se proyecta con estatus universitario: investigación, escritura académica, y práctica reflexiva. Muestran algunas fortalezas, dadas a partir de prácticas de docentes puntuales, y sobre todo la necesidad de impulsar estrategias formativas que habiliten y potencien el desarrollo de competencias en esas tres dimensiones, hoy muy escasas, tanto en estudiantes de último año de la carrera como en egresadas recientes.Agencia Nacional de Investigación e Innovació