Switching of macromolecular ligand display by thermoresponsive polymers mediates endocytosis of multi-conjugate nanoparticles

Ligand-mediated targeting and internalization of plasma membrane receptors is central to cellular function. These types of receptors have accordingly been investigated as targets to facilitate entry of diagnostic and therapeutic constructs into cells. However, there remains a need to characterize how receptor targeting agents on nanoparticles interact at surface receptors and whether it is possible to control these interactions via exogenous stimuli. Here, we describe the switchable display of the iron-transporting protein, transferrin (Tf), at the surface of thermoresponsive polymer-coated gold nanoparticles, and show that internalization of the coated nanoparticles into target cells changes across temperature ranges over which transferrin is expected to be sterically ‘hidden’ by an extended polymer chain and then ‘revealed’ by polymer chain collapse. The switching process is dependent on the numbers of transferrin molecules and thermoresponsive polymer chains attached, and whether the assay temperature is above or below the transition temperatures of the responsive polymers at the nanoparticle surfaces. Significantly, however, the control of internalization is critically reliant on overall nanoparticle colloidal stability while the thermoresponsive component of the surface undergoes conformational change. The data show that the cell entry function of complex and large biomolecule ligands can be modulated by polymer-induced accessibility change, but that a simple ‘hide and reveal’ mechanism for ligand display following polymer chain collapse is insufficient to account for nanoparticle uptake and subsequent intracellular trafficking

Tailoring Cosmopolitanism in the Italian Nordest

Cosmopolitanism has become a potent means through which the fashion industry captures value in the global economy. Recognizing the selling power of a cosmopolitan imaginary, the provincial clothing firms of North East Italy actively cultivate associations with global cities— drawing from their flow of people, cultures, images, and ideas—to absorb their urban edge and worldly aura. Located in predominantly rural areas far from established fashion centers, these firms symbolically capture the urbs through distribution, communication, and marketing strategies that endorse cities’ mythologies of modernity and excitement. Using stratagems centered around metropolitan cosmopolitanism, firms like Benetton and Diesel—prototypes of the industrial system of the region—skillfully transformed their labels into trendy "cosmobrands" and gained a central place in the topography of transnational fashion networks

Lockdowns

A short response to the sounds of lockdowns during a session of a Zoom Writing Salon initiated by Michelle Dent which involved eight former colleagues from the Department of Performance Studies at New York University

Introduction: Fashioning the Global City

Introduction to Streetnotes 20:  Fashioning the Global Cit

Tailoring Cosmopolitanism in the Italian Nordest

Cosmopolitanism has become a potent means through which the fashion industry captures value in the global economy. Recognizing the selling power of a cosmopolitan imaginary, the provincial clothing firms of North East Italy actively cultivate associations with global cities— drawing from their flow of people, cultures, images, and ideas—to absorb their urban edge and worldly aura. Located in predominantly rural areas far from established fashion centers, these firms symbolically capture the urbs through distribution, communication, and marketing strategies that endorse cities’ mythologies of modernity and excitement. Using stratagems centered around metropolitan cosmopolitanism, firms like Benetton and Diesel—prototypes of the industrial system of the region—skillfully transformed their labels into trendy "cosmobrands" and gained a central place in the topography of transnational fashion networks

Introduction: Fashioning the Global City

Introduction to Streetnotes 20:  Fashioning the Global Cit

Tailoring Cosmopolitanism in the Italian Nordest

Cosmopolitanism has become a potent means through which the fashion industry captures value in the global economy. Recognizing the selling power of a cosmopolitan imaginary, the provincial clothing firms of North East Italy actively cultivate associations with global cities— drawing from their flow of people, cultures, images, and ideas—to absorb their urban edge and worldly aura. Located in predominantly rural areas far from established fashion centers, these firms symbolically capture the urbs through distribution, communication, and marketing strategies that endorse cities’ mythologies of modernity and excitement. Using stratagems centered around metropolitan cosmopolitanism, firms like Benetton and Diesel—prototypes of the industrial system of the region—skillfully transformed their labels into trendy "cosmobrands" and gained a central place in the topography of transnational fashion networks

Streetnotes 20 Front Matter

Streetnotes 20 Front Matte