192 research outputs found
Comparative genomics reveals multiple pathways to mutualism for tick-borne pathogens
Accelerated pipeline for DNA and amino acid sequences clustering
Whole Proteome Clustering of 2,307 Proteobacterial Genomes Reveals Conserved Proteins and Significant Annotation Issues
We clustered 8.76 M protein sequences deduced from 2,307 completely sequenced Proteobacterial genomes resulting in 707,311 clusters of one or more sequences of which 224,442 ranged in size from 2 to 2,894 sequences. To our knowledge this is the first study of this scale. We were surprised to find that no single cluster contained a representative sequence from all the organisms in the study. Given the minimal genome concept, we expected to find a shared set of proteins. To determine why the clusters did not have universal representation we chose four essential proteins, the chaperonin GroEL, DNA dependent RNA polymerase subunits beta and beta′ (RpoB/RpoB′), and DNA polymerase I (PolA), representing fundamental cellular functions, and examined their cluster distribution. We found these proteins to be remarkably conserved with certain caveats. Although the groEL gene was universally conserved in all the organisms in the study, the protein was not represented in all the deduced proteomes. The genes for RpoB and RpoB′ were missing from two genomes and merged in 88, and the sequences were sufficiently divergent that they formed separate clusters for 18 RpoB proteins (seven clusters) and 14 RpoB′ proteins (three clusters). For PolA, 52 organisms lacked an identifiable sequence, and seven sequences were sufficiently divergent that they formed five separate clusters. Interestingly, organisms lacking an identifiable PolA and those with divergent RpoB/RpoB′ were predominantly endosymbionts. Furthermore, we present a range of examples of annotation issues that caused the deduced proteins to be incorrectly represented in the proteome. These annotation issues made our task of determining protein conservation more difficult than expected and also represent a significant obstacle for high-throughput analyses
Gene Duplicability-Connectivity-Complexity across Organisms and a Neutral Evolutionary Explanation
Gene duplication has long been acknowledged by biologists as a major evolutionary force shaping genomic architectures
and characteristics across the Tree of Life. Major research has been conducting on elucidating the fate of duplicated genes
in a variety of organisms, as well as factors that affect a geneï¾’s duplicabilityï¾–that is, the tendency of certain genes to retain
more duplicates than others. In particular, two studies have looked at the correlation between gene duplicability and its
degree in a protein-protein interaction network in yeast, mouse, and human, and another has looked at the correlation
between gene duplicability and its complexity (length, number of domains, etc.) in yeast. In this paper, we extend these
studies to six species, and two trends emerge. There is an increase in the duplicability-connectivity correlation that agrees
with the increase in the genome size as well as the phylogenetic relationship of the species. Further, the duplicabilitycomplexity
correlation seems to be constant across the species. We argue that the observed correlations can be explained
by neutral evolutionary forces acting on the genomic regions containing the genes. For the duplicability-connectivity
correlation, we show through simulations that an increasing trend can be obtained by adjusting parameters to approximate
genomic characteristics of the respective species. Our results call for more research into factors, adaptive and non-adaptive
alike, that determine a geneï¾’s duplicability
The characterization and manipulation of the bacterial microbiome of the Rocky Mountain wood tick, Dermacentor andersoni
BACKGROUND: In North America, ticks are the most economically impactful vectors of human and animal pathogens. The Rocky Mountain wood tick, Dermacentor andersoni (Acari: Ixodidae), transmits Rickettsia rickettsii and Anaplasma marginale to humans and cattle, respectively. In recent years, studies have shown that symbiotic organisms are involved in a number of biochemical and physiological functions. Characterizing the bacterial microbiome of D. andersoni is a pivotal step towards understanding symbiont-host interactions. FINDINGS: In this study, we have shown by high-throughput sequence analysis that the composition of endosymbionts in the midgut and salivary glands in adult ticks is dynamic over three generations. Four Proteobacteria genera, Rickettsia, Francisella, Arsenophonus, and Acinetobacter, were identified as predominant symbionts in these two tissues. Exposure to therapeutic doses of the broad-spectrum antibiotic, oxytetracycline, affected both proportions of predominant genera and significantly reduced reproductive fitness. Additionally, Acinetobacter, a free-living ubiquitous microbe, invaded the bacterial microbiome at different proportions based on antibiotic treatment status suggesting that microbiome composition may have a role in susceptibility to environmental contaminants. CONCLUSIONS: This study characterized the bacterial microbiome in D. andersoni and determined the generational variability within this tick. Furthermore, this study confirmed that microbiome manipulation is associated with tick fitness and may be a potential method for biocontrol
Development of the OPgunâ„¢ for bombardment of animal tissues
A simple and inexpensive particle-bombardment device, the OPgunâ„¢, was constructed for the
delivery of DNA into animal tissues. This device is based on the particle-inflow gun first described
for plant-cell transfection. The delivery of tungsten particles into the epidermis of the mouse ear,
without the use of vacuum and without causing damage to the tissue, was demonstrated. The system
was also shown to be capable of inducing antibodies to a foreign gene in mice.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format
Identification of Anaplasma marginale Type IV Secretion System Effector Proteins
Anaplasma marginale, an obligate intracellular alphaproteobacterium in the order Rickettsiales, is a tick-borne pathogen and the leading cause of anaplasmosis in cattle worldwide. Complete genome sequencing of A. marginale revealed that it has a type IV secretion system (T4SS). The T4SS is one of seven known types of secretion systems utilized by bacteria, with the type III and IV secretion systems particularly prevalent among pathogenic Gram-negative bacteria. The T4SS is predicted to play an important role in the invasion and pathogenesis of A. marginale by translocating effector proteins across its membrane into eukaryotic target cells. However, T4SS effector proteins have not been identified and tested in the laboratory until now.Published copyLockwood, S., D. E. Voth, K. A. Brayton, P. A. Beare, W. C. Brown, R. A. Heinzen, and S. L. Broschat, Identification of Anaplasma marginale type IV secretion system effector proteins, PLoS ONE, Vol. 6, No. 11, e7724, Nov. 2011. DOI: 10.1371/journal.pone.0027724
Cowdria ruminantium DNA is unstable in a SuperCos1 library
A Cowdria ruminantium genomic library was constructed in a cosmid vector to serve as a source of
easily accessible and pure C. ruminantium DNA for molecular genetic studies. The cosmid library
contained 846 clones which were arrayed into microtitre plates. Restriction enzyme digestion patterns
indicated that these clones had an average insert size of 35 kb. Probing of the arrays did not
detect any bovine clones and only one of the known C. ruminantium genes, pCS20, was detected.
Due to the high AT content and the fact that C. ruminantium genes are active in the Escherichia coli
host, the C. ruminantium clones were unstable in the SuperCos 1 vector and most clones did not grow
reproducibly. The library was contaminated with E. coli clones and these clones were maintained with
greater fidelity than the C. ruminantium clones, resulting in a skewed representation over time. We
have isolated seven C. ruminantium clones which we were able to serially culture reproducibly; two
of these clones overlap. These clones constitute the first large regions of C. ruminantium DNA to be
cloned and represent almost 10% of the C. ruminantium genome.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi.
Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201
Anaplasma marginale outer membrane protein vaccine candidates are conserved in North American and South African strains
Bovine anaplasmosis is a globally economically important tick-borne disease caused by the obligate intraerythrocytic rickettsia, Anaplasma marginale. A live Anaplasma centrale blood-based vaccine is available, but it does not protect against all A. marginale field strains and may also transmit other blood-borne pathogens. Five potential outer membrane protein (OMP) vaccine candidates have been well-characterised in A. marginale strains from the USA, however, their levels of conservation in other countries must be ascertained in order to inform their use in a vaccine with regional or global efficacy. This study assessed the amino acid variation in vaccine candidate OMPs in South African strains of A. marginale, and also compared the immunogenic properties between South African and US strains. OMP genes Am779, Am854, omp7, omp8 and omp9 were amplified and sequenced from a set of genetically diverse South African samples with different msp1α-genotypes. OMPs Am854 and Am779 were highly conserved, with 99–100 % amino acid identity, while Omp7, Omp8 and Omp9 had 79–100 % identity with US strains. As has been shown previously, Omp7–9 possess conserved N- and C- termini, a central variable region, and a highly conserved CD4 T-cell epitope, FLLVDDA(I/V)V, in the N-terminal region. Western blot analysis of recombinant OMPs indicates strong antigenic conservation between South African and US strains of A. marginale, suggesting that they are good candidates for use in a novel global vaccine cocktail, although further work on the best formulation and delivery methods will be necessary.The National Research Foundation (NRF) (Nicola Collins, grant number 81840) and Technology Innovation Agency, Tshwane Animal Health Cluster (Marinda Oosthuizen, grant number TAHC12-00037).http://www.elsevier.com/locate/ttbdis2021-04-18hj2020Veterinary Tropical Disease
Characterization of Anaplasma marginale subsp. centrale strains by use of msp1aS genotyping reveals a wildlife reservoir
Bovine anaplasmosis caused by the intraerythrocytic rickettsial pathogen Anaplasma marginale is endemic in South Africa.
Anaplasma marginale subspecies centrale also infects cattle; however, it causes a milder form of anaplasmosis and is used as a
live vaccine against A. marginale. There has been less interest in the epidemiology of A. marginale subsp. centrale, and, as a result,
there are few reports detecting natural infections of this organism. When detected in cattle, it is often assumed that it is
due to vaccination, and in most cases, it is reported as coinfection with A. marginale without characterization of the strain. A
total of 380 blood samples from wild ruminant species and cattle collected from biobanks, national parks, and other regions of
South Africa were used in duplex real-time PCR assays to simultaneously detect A. marginale and A. marginale subsp. centrale.
PCR results indicated high occurrence of A. marginale subsp. centrale infections, ranging from 25 to 100% in national parks.
Samples positive for A. marginale subsp. centrale were further characterized using the msp1aS gene, a homolog of msp1 of A.
mar-ginale, which contains repeats at the 5= ends that are useful for genotyping strains. A total of 47 Msp1aS repeats were
identified, which corresponded to 32 A. marginale subsp. centrale genotypes detected in cattle, buffalo, and wildebeest.
RepeatAnalyzer was used to examine strain diversity. Our results demonstrate a diversity of A. marginale subsp. centrale strains
from cattle and wildlife hosts from South Africa and indicate the utility of msp1aS as a genotypic marker for A. marginale
subsp. centrale strain diversity.http://jcm.asm.org2017-04-30hb2017Veterinary Tropical Disease
Comparison of three nucleic acid-based tests for detecting Anaplasma marginale and Anaplasma centrale in cattle
Several nucleic acid-based assays have been developed for detecting Anaplasma marginale and
Anaplasma centrale in vectors and hosts, making the choice of method to use in endemic areas
difficult. We evaluated the ability of the reverse line blot (RLB) hybridisation assay, two nested
polymerase chain reaction (nPCR) assays and a duplex real-time quantitative polymerase
chain reaction (qPCR) assay to detect A. marginale and A. centrale infections in cattle (n = 66) in
South Africa. The lowest detection limits for A. marginale plasmid DNA were 2500 copies by
the RLB assay, 250 copies by the nPCR and qPCR assays and 2500, 250 and 25 copies of
A. centrale plasmid DNA by the RLB, nPCR and qPCR assays respectively. The qPCR assay
detected more A. marginale- and A. centrale-positive samples than the other assays, either as
single or mixed infections. Although the results of the qPCR and nPCR tests were in agreement
for the majority (38) of A. marginale-positive samples, 13 samples tested negative for A. marginale
using nPCR but positive using qPCR. To explain this discrepancy, the target sequence region
of the nPCR assay was evaluated by cloning and sequencing the msp1β gene from selected field
samples. The results indicated sequence variation in the internal forward primer (AM100) area
amongst the South African A. marginale msp1β sequences, resulting in false negatives. We
propose the use of the duplex qPCR assay in future studies as it is more sensitive and offers the
benefits of quantification and multiplex detection of both Anaplasma spp.The
National Research Foundation (NRF) of South Africa
(grant number 81840 awarded to Dr Nicola Collins) and
Technology Innovation Agency (TIA), Tshwane Animal
Health Cluster (grant TAHC12-00037 awarded to Professor
Marinda Oosthuizen).http://www.ojvr.org/am2017GeneticsVeterinary Tropical Disease
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