Reef Rescue Marine Monitoring Program: Using remote sensing for GBR-wide water quality. Final report for 2012/13 activities

This report delivers management relevant information of flood events and inshore water quality compliance based on tailored temporal and spatial analysis of remote sensing data, carried out by CSIRO as part of the Reef Rescue Marine Monitoring Program (MMP) from 2005 to 2013

Reef Rescue Marine Monitoring Program: Assessment of terrestrial run-off entering the Reef and inshore marine water quality monitoring using earth observation data. Final report for 2010/11 activities

This report delivers management relevant information of flood events and inshore water quality compliance based on tailored temporal and spatial analysis of remote sensing data, carried out by CSIRO as part of the Reef Rescue Marine Monitoring Program (MMP) from 2005 to 2010

Reef Rescue Marine Monitoring Program: Using remote sensing for GBR-wide water quality. Final report for 2011/12 activities

This report delivers management relevant information of flood events and inshore water quality compliance based on tailored temporal and spatial analysis of remote sensing data, carried out by CSIRO as part of the Reef Rescue Marine Monitoring Program (MMP) from 2005 to 2013

Development and validation of a cell based model of insulin resistance and investigation into the intracellular molecular defects induced by diabetes and obesity

A reduction in the sensitivity of tissue to insulin is termed insulin resistance. In the clinic this condition is associated with obesity and inactivity and often leads to the development of type 2 diabetes. A major focus of antidiabetic therapy is to develop novel interventions to alleviate insulin resistance. However, the initial physiological and molecular defects in the development of insulin resistance remain elusive. This knowledge would greatly aid the development of novel and more effective insulin sensitisers.In an effort to improve the understanding of insulin resistance this thesis establishes that culturing liver cells in sera from obese diabetic patients reduces the ability of insulin to repress the key gluconeogenic gene, phosphoenolpyruvatecarboxykinase (PEPCK). Cells cultured in serum from obese diabetic human subjects exhibited defective PEPCK mRNA suppression by 0.1 and 0.5 nM insulin compared to cells cultured in control serum (p<0.0001), representing a shift to the right of the insulin dose response curve. Classification of human sera, using the response of the cell model following incubation with the sera, was actually more reliable than any single clinical biomarker at establishing whether the serum came from a volunteer with insulin resistance. This suggests that the cell model could be developed as a means to classify insulin resistance in the human population more reliably than simply measuring fasting glucose.The system was developed and optimised as a cell based humanised model of insulin resistance to aid the search for a biomarker for the development of obesity related insulin resistance. However, there was no linear relationship between any single biomarker and the resistance causing ability of the sera. Interestingly, cells cultured chronically in the presence of fetal calf serum supplemented with 5 pM insulin (the average increase in insulin between cases and controls) also exhibited reduced suppression of PEPCK by 0.1 and 0.5 nM insulin compared to controls (p=0.03 and 0.01 respectively). This has major implications for the understanding of how insulin resistance may develop. It suggests that minor increases in insulin release from beta cells, or minor loss of insulin clearance in the liver that elevate plasma insulin are potential initiating mechanisms for insulin resistance (at least in liver). Of course there may be many ways to initiate insulin resistance in vivo, but establishing the relative importance of the beta cell and the liver as an initial site for the development of insulin resistance is clearly important for effective intervention. Subsequent to the generation of insulin resistance in culture I could not detect significant differences in the response of the major post-receptor insulin signalling pathway components, between cells cultured under standard conditions and those cultured chronically in 5 pM insulin. Therefore the mechanism underlying this reduced insulin action on PEPCK gene transcription remains unclear.I then went on to develop reporter cell lines both for use in the study of the regulation of hepatic gene transcription by insulin and also as a potential screen for effective insulin sensitisers. Unfortunately the reporter cell lines did not turn out to be useful as hoped, as the reporter genes did not develop insulin resistance in response to chronic exposure to 5 pM insulin. In addition there were some differences between the reporter genes and endogenous genes in response to specific signalling inhibitors. This questions their suitability for the purposes proposed.Finally, I examined the signalling connections between the class of insulin sensitiser known as biguanides, and DNA repair mechanisms, as an initial characterisation of molecular links between diabetes and cancer. I established that inhibiting the DNA repair enzyme ATM reduces the phosphorylation of the biguanide target, AMPK in response to these drugs. However, although inhibition of ATM reduced biguanide suppression of G6Pase it had little effect on the regulation of PEPCK gene transcription by the drugs. This is consistent with AMPK not being the key mediator of biguanide regulation of PEPCK gene transcription and suggests that biguanide regulation of G6Pase and PEPCK gene transcription is mediated through distinct signalling pathways.In summary, I have developed a cell based model of insulin resistance that relies on factor (s) present in serum from humans with diabesity, and thus should be useful as a screen for more effective insulin sensitisers targeted at the population that donates the serum. It is likely that one of the factors responsible for generation of resistance is insulin itself as chronic exposure to low levels (albeit higher than background), of insulin reduces insulin sensitivity of the cells. The molecular details of the development of insulin resistance remain elusive as none of the major signalling pathways appear to be defective in the cells that have developed reduced insulin regulation of PEPCK. However, the data raise the intriguing possibility that chronic but mild hyperinsulinemia due to defective insulin secretion or clearance is an initial step in the development of insulin resistance. Hence, reducing insulin secretion (as opposed to current strategies of inducing insulin secretion) may be a more effective therapy for prevention of the development of insulin resistance. Finally, elements of the DNA repair pathways such as ATM may impinge on pathways that affect insulin sensitivity, including the biguanide target AMPK.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Assessment of environmental management effects in a shallow water basin using mass-balance models.

Comparative analysis of trophic networks was carried out to evaluate environmental management actions aimed at countering an environmental crisis in Orbetello Lagoon, Italy. Two mass-balance models of this shallow water coastal system were constructed, for 1995 and 1996. During this period, there was an observed change in the composition of the submerged vegetation that indicated a significant improvement in the lagoon’s ecology. Mass-balance models were built using the Ecopath modelling software in order to explain the energy transfer through the trophic levels (TLs) of the lagoon’s ecosystem. Comparative analysis of the two trophic networks allowed a complete description of the lagoon, and gave clear indications regarding the ‘eutrophication level’ sensu Nixon and the stage of system maturity sensu Odum of the ecosystem for the 2 years. The turnover rate (production/biomass ratio) of pleustophytes was proposed as an indicator of “environmental health” at the ecosystem level because it allows positioning the submerged vegetation as a whole along an axis of adaptation strategies to natural selection. All the indices of ecosystem maturity and stability examined showed that the lagoon was in a more stable condition in 1996 than in 1995, although the system was still in a condition of stress. The effects of management actions carried out in the system were quantified by estimating the changes in primary production and accumulation of detritus in the system. Management of the lagoon as an extensive aquaculture operation was assessed by analysing the fishery catch, the transfer efficiencies at different trophic levels and the impact of cormorants, and it was found to contribute to system stability

Satellite data assimilation and estimation of a 3D coastal sediment transport model using error-subspace emulators

This paper describes sequential assimilation of data into a three-dimensional coastal ocean model using fast and cheap statistical surrogates of the model (emulators). The model simulates resuspension and deposition of fine sediments in a macro-tidal environment of the Fitzroy Estuary and Keppel Bay, North-East Australian coast. The assimilation algorithm was applied first to synthetic observations produced by a twin model run, and then with real data obtained from satellite observation. The latter are derived from remote sensing algorithms customised to the study region. The main objective of simulations was to test the data assimilation scheme using synthetic observations and identify potential issues and challenges when assimilating real data sets. The assimilation algorithm proved capable of substantially reducing a prior uncertainty of the model for both the scenario with the synthetic observations and the scenario with the satellite data. Significant remaining error in western Keppel Bay after assimilating satellite data is diagnostic of an underlying error in the system conceptualisation in other words, it indicates that the primary source of error is not in the parameter values specified, but in the model structure, in the interpretation of satellite data or in the other input data. The results of our study show the utility of the developed technique for the data assimilation into the three-dimensional sediment transport model of the Fitzroy estuary and Keppel Bay. More research is required to understand the capacity of this technique to generalise to other models and regions. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved

Assessment and treatment of malignant pleural effusions : visual analogue scale, ultrasound and drainage

This thesis consists of 3 studies: 1. Determination of the minimal important difference (MID) of the visual analogue scale for dyspnoea (VASD): Determining the MID of the VASD is essential to interpret the results of trials in patients with malignant pleural effusions (MPEs). Patients undergoing a pleural procedure assessed the change in their VASD and the degree of change in their symptoms on a Likert scale. The mean VASD in patients experiencing a ‘small but just worthwhile’ decrease in their symptoms is the MID for the VASD and was found to be 22mm (95% CI 16 - 27mm). 2. Development of a thoracic ultrasound septation score (TUSS): A TUSS is important for objectively assessing the degree of septation within a pleural effusion. An iterative process was used to demonstrate that degree of septation predicts clinical outcome, to identify candidate factors for inclusion in a TUSS and to determine which factors predicted the degree of septation. The final TUSS consisted of an assessment of the degree of homogeneity of septation distribution and number of septations at the most septated area. 3. Effect of an indwelling pleural catheter (IPC) versus standard care for relieving dyspnoea in patients with MPEs: the TIME2 randomised controlled trial (RCT). The objective of this unblinded RCT was to determine whether IPCs are more effective than chest drains and talc pleurodesis at relieving dyspnoea in patients with MPEs. 106 patients were randomised to either IPC or standard care in a 1:1 ratio. The primary outcome was daily VASD over 42 days post intervention. Dyspnoea improved in both groups with no significant difference in mean dyspnoea in the first 42 days (mean score: IPC 25mm (95% CI 19 – 30), standard care 24mm (95% CI 19 – 29)).EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Long term simulations of population dynamics of Ulva r. in the lagoon of Venice

The dynamic of macroalgae is implemented in a 3D transport-water-quality model of the central part of the lagoon of Venice. Ulva biomass density and nitrogen concentration in Ulva tissue have been added to the set of state variables previously considered, that is to phytoplankton and zooplankton densities, concentrations of nutrients in water, detritus and dissolved oxygen. The model shows that Ulva succeeds in the competition with the phytoplanktonic community in the shallower areas, where water temperature and irradiance levels reaching the bottom are sufficient to sustain growth. Long term evolutions of Ulva colonies, under different scenarios of forcing functions, show that adverse meteoclimatic conditions can be more effective in reducing Ulva biomass than a consistent decrease in the loads of Nitrogen