1,031 research outputs found

    Structural Analysis of Molecular Chaperones: Nucleotide Exchange Factors of Hsp70 and the Assembly Chaperone RbcX

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    Survey of current Swiss pig feeding practices and potential for ammonia emission reduction

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    Controlling potentially harmful and polluting emissions from farms is important in the developed world, where legislation exists in many countries limiting emissions such as ammonia and controlling how manure is disposed of from intensive farming operations. In Switzerland, there are legal agreements concerning controls of ammonia emissions, most especially from farms. Ammonia production from pig farms can be controlled by dietary intervention, such as reducing protein levels, which in turn reduces excretion, mainly via urine. The following paper surveys current practices for nitrogen use on Swiss pig farms, and how feeding strategies may assist in controlling ammonia production from pig production systems. The survey found that 70-75% of all feeds used for pigs of all categories were reduced in protein and nitrogen, with 90% being reduced in protein in high animal density areas. Regression analysis showed that CP levels explained up to 49% of the nitrogen efficiency, suggesting that other factors are important in pollution control. Although piglet diets are more tightly regulated in terms of controlling N input, excessive protein levels in so-called reduced protein diets for finisher pigs and dry sows are common in the market. Hence, there is considerable potential to reduce N-input and ammonia emissions from Swiss pig production, which could be implemented at no or minimal extra cos

    Survey of current Swiss pig feeding practices and potential for ammonia emission reduction

    Get PDF
    Controlling potentially harmful and polluting emissions from farms is important in the developed world, where legislation exists in many countries limiting emissions such as ammonia and controlling how manure is disposed of from intensive farming operations. In Switzerland, there are legal agreements concerning controls of ammonia emissions, most especially from farms. Ammonia production from pig farms can be controlled by dietary intervention, such as reducing protein levels, which in turn reduces excretion, mainly via urine. The following paper surveys current practices for nitrogen use on Swiss pig farms, and how feeding strategies may assist in controlling ammonia production from pig production systems. The survey found that 70-75% of all feeds used for pigs of all categories were reduced in protein and nitrogen, with 90% being reduced in protein in high animal density areas. Regression analysis showed that CP levels explained up to 49% of the nitrogen efficiency, suggesting that other factors are important in pollution control. Although piglet diets are more tightly regulated in terms of controlling N input, excessive protein levels in so-called reduced protein diets for finisher pigs and dry sows are common in the market. Hence, there is considerable potential to reduce N-input and ammonia emissions from Swiss pig production, which could be implemented at no or minimal extra cost

    The nucleotide exchange factors of Hsp70 molecular chaperones.

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    Molecular chaperones of the Hsp70 family form an important hub in the cellular protein folding networks in bacteria and eukaryotes, connecting translation with the downstream machineries of protein folding and degradation. The Hsp70 folding cycle is driven by two types of cochaperones: J-domain proteins stimulate ATP hydrolysis by Hsp70, while nucleotide exchange factors (NEFs) promote replacement of Hsp70-bound ADP with ATP. Bacteria and organelles of bacterial origin have only one known NEF type for Hsp70, GrpE. In contrast, a large diversity of Hsp70 NEFs has been discovered in the eukaryotic cell. These NEFs belong to the Hsp110/Grp170, HspBP1/Sil1, and BAG domain protein families. In this short review we compare the structures and molecular mechanisms of nucleotide exchange factors for Hsp70 and discuss how these cochaperones contribute to protein folding and quality control in the cell

    Remote sensing of coccolithophore blooms in selected oceanic regions using the PhytoDOAS method applied to hyper-spectral satellite data

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    In this study temporal variations of coccolithophore blooms are investigated using satellite data. Eight years (from 2003 to 2010) of data of SCIAMACHY, a hyper-spectral satellite sensor on-board ENVISAT, were processed by the PhytoDOAS method to monitor the biomass of coccolithophores in three selected regions. These regions are characterized by frequent occurrence of large coccolithophore blooms. The retrieval results, shown as monthly mean time series, were compared to related satellite products, including the total surface phytoplankton, i.e. total chlorophyll a (from GlobColour merged data) and the particulate inorganic carbon (from MODIS-Aqua). The inter-annual variations of the phytoplankton bloom cycles and their maximum monthly mean values have been compared in the three selected regions to the variations of the geophysical parameters: sea-surface temperature (SST), mixed-layer depth (MLD) and surface wind-speed, which are known to affect phytoplankton dynamics. For each region, the anomalies and linear trends of the monitored parameters over the period of this study have been computed. The patterns of total phytoplankton biomass and specific dynamics of coccolithophore chlorophyll a in the selected regions are discussed in relation to other studies. The PhytoDOAS results are consistent with the two other ocean color products and support the reported dependencies of coccolithophore biomass dynamics on the compared geophysical variables. This suggests that PhytoDOAS is a valid method for retrieving coccolithophore biomass and for monitoring its bloom developments in the global oceans. Future applications of time series studies using the PhytoDOAS data set are proposed, also using the new upcoming generations of hyper-spectral satellite sensors with improved spatial resolution

    Effects of a β-andrenergic agonist on growth performance, body composition and nutrient retention in finishing pigs fed normal or low amounts of protein

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    In earlier studies with pigs the P-adrenergic agonist Ro 16·8714 ((3-AG) enhanced the efficiency of nitrogen (N) retention. Therefore effects of Ro 16·8714 were studied on growth rate, body composition, N, fat and energy retention in pigs fed isoenergetically, but given different amounts of protein (112 or 138 g/kg diet) without (groups LP and NP) or with 60 mg Ro 16·8714 per kg diet (groups LPP and NPP) from 60 to 100 kg live weight. Weight gain (898, 927, 855 and 810 g/day in NP, NPp, LP and LPp) decreased, whereas food: gain ratio (2·94, 2·82, 3·04 and 3·24 kg/kg in NP, NPP, LP and LPP) was increased by low protein intake (P 0·05) whereas the efficiency of energy retention was not influenced by Ro 16·8714 and protein intake. In conclusion, an adequate intake of protein is necessary for optimum expression of many, but not all, effects of the P-adrenergic agonist Ro 16·871
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