Chlorella vulgaris biomass enriched by biosorption of polyphenols

Cell walls of microalgae are variable and contain non-specific domains where different molecules can bind. The enrichment of microalgal biomass with nutrients through adsorption can be an interesting process for the food and feed industry. In this study, naturally occurring polyphenols ((+)-catechin, ()-epicatechin, quercetin, rutin and xanthohumol) were adsorbed onto nonliving cells of freshwater microalgae Chlorella vulgaris. The essential adsorption parameters such as biomass dose and contact time were examined and the adsorption was quantified with Langmuir, Sips and DubininRadushkevich adsorption isotherms. The evaluation of isotherms proved the highest affinity towards Chlorella vulgaris biomass for xanthohumol and quercetin. The biosorption mechanism of Chlorella vulgaris biomass was well described by a pseudo second order kinetic model, with a high regression coefficient. The polyphenol-enriched microalgal biomass was also evaluated for its antioxidant activity. The highest antioxidant activity was detected in the case of biomass enriched with quercetin (77.5% of decolorized DPPH).The financial support by the Ministry of Education, Youth and Sports of the Czech Republic through grant MSM6046137305 is gratefully acknowledged. Cristina Quintelas is thankful to Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) for funding the CEB researcher unit at the University of Minho and thanks the Project "BioInd - Biotechnology and Bioengineering for improved Industrial and Agro-Food processes, REF. NORTE-07-0124-FEDER-000028" co-funded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN and FEDER

"Aunt Julia" and Vargas Llosa: Literary character of two autobiographies

Práce se zaměří na porovnání autobiografického románu Maria Vargase Llosy Tetička Julie a zneuznaný génuis (La Tía Julia y el escribidor, 1977), který z jedné poloviny vypráví o manželství s jeho "tetičkou" a druhou polovinu tvoří smyšlené rozhlasové příběhy, a autobiografického textu Co Varguitas neřekl (Lo que Varguitas no dijo, 1983), který je reakcí na tuto knihu, ve které autorka Julia Urquidi sepsala svou vlastní verzi vztahu s Mariem Vargasem Llosou. Práce se soustřeďuje na odlišnosti těchto dvou knih, které vypráví o tom samém vztahu, ovšem z jiného úhlu pohledu. Rozebere literární charakter obou děl a spolu s ním i úmysl obou autorů při výstavbě svých knih. Práce u obou textů analyzuje jejich kompozici, čas, vypravěče a styl. Zejména pak u díla Tetička Julie a zneuznaný génius se soustředí na autorovu hru s autobiografickými a fikčními prvky. Práce se pokouší najít odpověď na otázku, jak moc je fikce kombinovatelná se skutečností a do jaké míry ji lze použít v autobiografickém textu a naopak. Kromě toho práce také rozebírá text Julie Urquidi a zasazuje ho do kontextu díla Vargase Llosy.The work will focus on the comparison of Maria Vargas Llosa's autobiographical novel Aunt Julia and the Scriptwriter (La Tía Julia y el escribidor, 1977), where its one half tells a story of his marriage to his "aunt" and the other half presents fictional radio stories, and the autobiographical text What Varguitas didn't say (Lo que Varguitas no dijo, 1983), which is a response to this book, in which the author Julia Urquidi wrote her own version of the relationship with Mario Vargas Llosa. The work focuses on the differences between these two books, which tell the story of the same relationship, but from a different perspective. It will analyze the literary character of both works and with it the intention of both authors during the construction of their books. The work will analyze the composition, time, narrator and style of both books. Especially in the novel Aunt Julia and the Scriptwriter, it focuses on the author's play with autobiographical and fictional elements. The work tries to find an answer to the question of how much fiction can be combined with reality and to what extent it can be used in an autobiographical text and vice versa. The work also analyzes the text of Julia Urquidi and places it in the context of the work of Mario Vargas Llosa.Ústav románských studiíInstitute of Romance StudiesFaculty of ArtsFilozofická fakult

Experimental Plan for the Project: Electrocoagulation Unit for Microalgal Biomass Separation.

Electrofloculation (electrocoagulation) of microalgae is a highly complex process involving a number of very complex transport, electrochemical, hydrodynamic and surface phenomena. The description of this process at the level of single mechanisms involved is too complicated and experimentally demanding, fortunately it is not absolutely necessary for the practical application. The electrofloculation was therefore approached by an engineering method that identified the criteria (range) of the desired variables and the main factors that affect them. For these factors, partial factorial experimental plans were designed

Experimental Report, Period 9-12/2017, Project: Electrocoagulation Unit for Microalgal Biomass Separation.

Production of microalgae in the world is increasing every year, but their use in food, cosmetics, feed and biofuel production is still limited by the high production price and high energy input. The substantial part of the energy costs lies in the separation of algal biomass from the medium, which is most often performed on plate centrifuges. The harvest concentration of algal suspensions in most cultivation systems ranges between just 1-5 g/L. Therefore, to obtain 1 kg of dry biomass, it is necessary to remove 200 to 1000 liters of water (by centrifuging and then drying). This amount of water and thus the energy input can be reduced via flocculation added as a pre-step prior to centrifugation. Electrofloculation is type of floculation, where the flocculant is directly formed by dissolving the electrodes. Suitable electrode material and parameter set enable obtaining of biomass, which is not contaminated by chemical flocculants and meets requirements for food stuff. The main objective of this project is to develop a continuous electroflocculation device suitable for these purposes.\

"Aunt Julia" and Vargas Llosa: Literary character of two autobiographies

Práce se zaměří na porovnání autobiografického románu Maria Vargase Llosy Tetička Julie a zneuznaný génuis (La Tía Julia y el escribidor, 1977), který z jedné poloviny vypráví o manželství s jeho "tetičkou" a druhou polovinu tvoří smyšlené rozhlasové příběhy, a autobiografického textu Co Varguitas neřekl (Lo que Varguitas no dijo, 1983), který je reakcí na tuto knihu, ve které autorka Julia Urquidi sepsala svou vlastní verzi vztahu s Mariem Vargasem Llosou. Práce se soustřeďuje na odlišnosti těchto dvou knih, které vypráví o tom samém vztahu, ovšem z jiného úhlu pohledu. Rozebere literární charakter obou děl a spolu s ním i úmysl obou autorů při výstavbě svých knih. Práce u obou textů analyzuje jejich kompozici, čas, vypravěče a styl. Zejména pak u díla Tetička Julie a zneuznaný génius se soustředí na autorovu hru s autobiografickými a fikčními prvky. Práce se pokouší najít odpověď na otázku, jak moc je fikce kombinovatelná se skutečností a do jaké míry ji lze použít v autobiografickém textu a naopak. Kromě toho práce také rozebírá text Julie Urquidi a zasazuje ho do kontextu díla Vargase Llosy.The work will focus on the comparison of Maria Vargas Llosa's autobiographical novel Aunt Julia and the Scriptwriter (La Tía Julia y el escribidor, 1977), where its one half tells a story of his marriage to his "aunt" and the other half presents fictional radio stories, and the autobiographical text What Varguitas didn't say (Lo que Varguitas no dijo, 1983), which is a response to this book, in which the author Julia Urquidi wrote her own version of the relationship with Mario Vargas Llosa. The work focuses on the differences between these two books, which tell the story of the same relationship, but from a different perspective. It will analyze the literary character of both works and with it the intention of both authors during the construction of their books. The work will analyze the composition, time, narrator and style of both books. Especially in the novel Aunt Julia and the Scriptwriter, it focuses on the author's play with autobiographical and fictional elements. The work tries to find an answer to the question of how much fiction can be combined with reality and to what extent it can be used in an autobiographical text and vice versa. The work also analyzes the text of Julia Urquidi and places it in the context of the work of Mario Vargas Llosa.Ústav románských studiíInstitute of Romance StudiesFaculty of ArtsFilozofická fakult

Feminism and femininity in the poems of Alfonsina Storni

The work focuses on the work of Argentinean author Alfonsina Storni, especially in connection with the new wave of feminism at the beginning of the 20th century. The chosen topic will be set in a wider context, including social conditions, political situation and society's opinions of female authors at the beginning of the twentieth century in Latin America. All these factors will then be used in a detailed analysis of the author's verses. The aim of the work is to define the style of Alfonsina Storni and, based on the content of her poems, to get an overview of her femininity, her relationship to men and how she succeeded in linking modernism with feminism. To all this I will also add the differences between feminism in the life of Alfonsina Storni and the one of today. All this on the basis of her work. Key words: Alfonsina Storni, poetry, feminism, Argentina

Feminism and femininity in the poems of Alfonsina Storni

Práce se soustřeďuje na tvorbu argentinské autorky Alfonsiny Storni, a to hlavně ve spojitosti s tehdejší vlnou feminismu. Zvolené téma bude zasazeno do širšího kontextu, to znamená včetně sociálních podmínek, politické situace a názorů společnosti na ženské autorky na začátku dvacátého století v Latinské Americe. Všechny tyto faktory budou následně použity v detailní analýze autorčiných veršů. Cílem práce je blíže definovat styl Alfonsiny Storni a na základě obsahu jejich básní získat přehled o tom, jak se v něm projevuje její ženskost, její vztah k mužům a jak se jí podařilo v jejich dílech propojit modernismus s feminismem. Do toho všeho zapojím rozdíly mezi feminismem v době života Alfosiny Storni a tím dnešním. To vše na základě její tvorby.The work focuses on the work of Argentinean author Alfonsina Storni, especially in connection with the new wave of feminism at the beginning of the 20th century. The chosen topic will be set in a wider context, including social conditions, political situation and society's opinions of female authors at the beginning of the twentieth century in Latin America. All these factors will then be used in a detailed analysis of the author's verses. The aim of the work is to define the style of Alfonsina Storni and, based on the content of her poems, to get an overview of her femininity, her relationship to men and how she succeeded in linking modernism with feminism. To all this I will also add the differences between feminism in the life of Alfonsina Storni and the one of today. All this on the basis of her work. Key words: Alfonsina Storni, poetry, feminism, Argentina.Ústav románských studiíInstitute of Romance StudiesFilozofická fakultaFaculty of Art

"Aunt Julia" and Vargas Llosa: Literary character of two autobiographies

The work will focus on the comparison of Maria Vargas Llosa's autobiographical novel Aunt Julia and the Scriptwriter (La Tía Julia y el escribidor, 1977), where its one half tells a story of his marriage to his "aunt" and the other half presents fictional radio stories, and the autobiographical text What Varguitas didn't say (Lo que Varguitas no dijo, 1983), which is a response to this book, in which the author Julia Urquidi wrote her own version of the relationship with Mario Vargas Llosa. The work focuses on the differences between these two books, which tell the story of the same relationship, but from a different perspective. It will analyze the literary character of both works and with it the intention of both authors during the construction of their books. The work will analyze the composition, time, narrator and style of both books. Especially in the novel Aunt Julia and the Scriptwriter, it focuses on the author's play with autobiographical and fictional elements. The work tries to find an answer to the question of how much fiction can be combined with reality and to what extent it can be used in an autobiographical text and vice versa. The work also analyzes the text of Julia Urquidi and places it in the context of the work of Mario Vargas Llosa