20 research outputs found
Sterilization of ophthalmic drugs and intraocular lenses
Poster presented at the First International Congress of CiiEM - From Basic Sciences to Clinical Research. 27-28 November 2015, Egas Moniz, Caparica, PortugalFundação para a Ciência e a Tecnologia: projects UID/QUI/00100/2013 and M-ERA.NET/0005/201
Spatial and single-cell profiling of the metabolome, transcriptome and epigenome of the aging mouse liver
Tissues within an organism and even cell types within a tissue can age with different velocities. However, it is unclear whether cells of one type experience different aging trajectories within a tissue depending on their spatial location. Here, we used spatial transcriptomics in combination with single-cell ATAC-seq and RNA-seq, lipidomics and functional assays to address how cells in the male murine liver are affected by age-related changes in the microenvironment. Integration of the datasets revealed zonation-specific and age-related changes in metabolic states, the epigenome and transcriptome. The epigenome changed in a zonation-dependent manner and functionally, periportal hepatocytes were characterized by decreased mitochondrial fitness, whereas pericentral hepatocytes accumulated large lipid droplets. Together, we provide evidence that changing microenvironments within a tissue exert strong influences on their resident cells that can shape epigenetic, metabolic and phenotypic outputs
Surface-Initiated Polymer Brushes in the Biomedical Field: Applications in Membrane Science, Biosensing, Cell Culture, Regenerative Medicine and Antibacterial Coatings
Physicochemical and surface properties of acrylic intraocular lenses and their clinical significance
Avaliação histopatológica da cápsula posterior associada ao implante de lente intraocular com superfície modificada com plasma de flúor e polietilenoglicol em coelhos
Physicochemical and Biological Investigation of Different Structures of Carbon Coatings Deposited onto Polyurethane
Aging is associated with increased chromatin accessibility and reduced polymerase pausing in liver
Regulation of gene expression is linked to the organization of the genome. With age, chromatin alterations occur on all levels of genome organization, accompanied by changes in the gene expression profile. However, little is known about the changes in the level of transcriptional regulation. Here, we used a multi-omics approach and integrated ATAC-, RNA- and NET-seq to identify age-related changes in the chromatin landscape of murine liver and to investigate how these are linked to transcriptional regulation. We provide the first systematic inventory of the connection between aging, chromatin accessibility, and transcriptional regulation in a whole tissue. Aging in murine liver is characterized by an increase in chromatin accessibility at promoter regions, but not in an increase in transcriptional output. Instead, aging is accompanied by a decrease in promoter-proximal pausing of RNA polymerase II (Pol II), while initiation of transcription is not decreased as assessed by RNA polymerase mapping using CUT&RUN. Based on the data reported, we propose that these age-related changes in transcriptional regulation are due to a reduced stability of the pausing complex
Sterilization of intraocular lenses loaded with moxifloxacin
Poster presented at the ARVO 2016 Annual Meeting [Association for Research in Vision and Ophthalmology]. Seattle, USA, 1-5 May 2016N/