Genetics, physiology and cytology of yest-mycelial dimorphism in fission yeasts

The order Schizosaccharomycetalescontains a dimorphic and two yeast species. Sch. japonicuscan form both yeast cells and mycelium, depending on the substrate and the culturing conditions.Sch. pombeis a strictly unicellular organism, but it can be forced to form mycelial cell chains by inactivating members of thesepgene family. The mutations in most of thesepgenes confer pleitropic phenotypes indicating functional involvement in MAP-kinase-mediated signalling pathways. Two of them were found to encode transcription factor homologues of other eukaryotes

Thermal Manipulation of Human Bone Collagen Membrane (SoftBone) and Platelet-Rich Fibrin (PRF) Membranes

Resorbable barrier membranes, including platelet-rich fibrin (PRF) and collagen membranes, can play a key role in guided bone regeneration surgeries (GBR) in dentistry. A new collagen membrane made of partially decalcified allogeneic cortical bone, termed SoftBone membrane (SB), was produced by West Hungarian Regional Tissue Bank. It can be easily adapted to diverse surfaces. Fresh and freeze-dried folded-PRF membranes were compared with freeze-dried SB. Important properties of membranes were reported (moisture content, rehydration capacity, and resistance against proteolytic enzyme). The SB exhibited the best resistance against enzymatic digestion on day 21, its weight was 34% of the original. Fresh F-PRF (folded PRF) disintegrated on the 11th day, while the freeze-dried F-PRF membrane dissolved completely on day 8. The thermal manipulation of the F-PRF membrane using freeze-drying has advantages and also disadvantages in comparison to the fresh one