Determining the cellular targets and resulting pathology of rift valley fever virus infection of the rat CNS and reproductive system using microscopy

Rift Valley fever virus is an emerging zoonotic pathogen that infects domestic ruminants and causes fatal hepatic necrosis and “abortion storms” that result in loss of up to 90-100% of pregnancies in animals. Human infection occurs among individuals in contact with affected animals and typically manifests as a febrile illness that can advance to hemorrhagic fever or neurological disease. In patients that develop encephalitis, there is a 50% chance of death while survivors experience long-term complications. Human cases of vertical transmission during pregnancy resulted in fetal viremia, abnormalities, and death; however, the rates of congenital RVFV are misunderstood and understudied. Rodent encephalitic and congenital models of RVF have recently been developed and are crucial for elucidating the mechanism of infection separating febrile illness from severe outcomes effecting the central nervous and reproductive systems. Whole, homogenized tissues from these models have been analyzed for viral titers, infiltrating immune cells, and resulting pathologies; however, specific infection events occurring within precise tissue structures required immunofluorescence and chromogen staining for microscopic image analysis and scoring. Thin sections of tissues from both models were stained for viral and cellular markers and imaged in order to determine specific disease events observed in our lab’s previous studies, Albe et al. (2019) and McMillen et al. (2018). Immunofluorescence micrographs supported the presence of vRNA and inflammatory leukocyte infiltration in the olfactory bulbs and cortexes as early as 1 day post-infection (dpi), and massive neuronal infection and necrosis beginning by 3 dpi. End-stage neurological disease, beginning at 5 dpi, was represented by a massive blood brain barrier breakdown event and influx of inflammatory cells. Histological analysis of pregnant RVFV-infected rat tissues showed the highest amount of vRNA in the placental tissues rather than the liver, and moderate levels of virus in the uterus and ovaries. Within the placenta, RVFV targeted the maternal decidual layer and fetal basal and labyrinth zones. Viral signal was also detected in the placentas of dams that survived infection. Visual immunohistochemistry data, as developed and presented here, can help highlight structural and cellular targets of RVFV that should be focused on for therapeutic efforts. RVFV is in the Federal Select Agent program for pathogens that have the potential to post a severe threat to public, animal, or plant health, on the World Health Organization’s list of Blueprint priority diseases requiring an urgent need for accelerated research and development, and a category A high-priority pathogen of the National Institute of Allergy and Infectious Diseases, which are biological agents that post the highest risk to national security; therefore research into therapeutics for RVF is of major public health significance

Probing fundamental physics with pulsars

Pulsars provide a wealth of information about General Relativity, the equation of state of superdense matter, relativistic particle acceleration in high magnetic fields, the Galaxy's interstellar medium and magnetic field, stellar and binary evolution, celestial mechanics, planetary physics and even cosmology. The wide variety of physical applications currently being investigated through studies of radio pulsars rely on: (i) finding interesting objects to study via large-scale and targeted surveys; (ii) high-precision timing measurements which exploit their remarkable clock-like stability. We review current surveys and the principles of pulsar timing and highlight progress made in the rotating radio transients, intermittent pulsars, tests of relativity, understanding pulsar evolution, measuring neutron star masses and the pulsar timing array.Comment: 6 pages, 1 figure, to appear in the proceedings of IAU XXVII GA - JD3 - Neutron Stars: Timing in Extreme Environments XXVII IAU General Assembly, Rio de Janeiro, Brazil, 3-14 August 200

Reconstruction of photon statistics using low performance photon counters

The output of a photodetector consists of a current pulse whose charge has the statistical distribution of the actual photon numbers convolved with a Bernoulli distribution. Photodetectors are characterized by a nonunit quantum efficiency, i.e. not all the photons lead to a charge, and by a finite resolution, i.e. a different number of detected photons leads to a discriminable values of the charge only up to a maximum value. We present a detailed comparison, based on Monte Carlo simulated experiments and real data, among the performances of detectors with different upper limits of counting capability. In our scheme the inversion of Bernoulli convolution is performed by maximum-likelihood methods assisted by measurements taken at different quantum efficiencies. We show that detectors that are only able to discriminate between zero, one and more than one detected photons are generally enough to provide a reliable reconstruction of the photon statistics for single-peaked distributions, while detectors with higher resolution limits do not lead to further improvements. In addition, we demonstrate that, for semiclassical states, even on/off detectors are enough to provide a good reconstruction. Finally, we show that a reliable reconstruction of multi-peaked distributions requires either higher quantum efficiency or better capability in discriminating high number of detected photons.Comment: 8 pages, 3 figure

Orion Aerodynamics for Hypersonic Free Molecular to Continuum Conditions

Numerical simulations are performed for the Orion Crew Module, previously known as the Crew Exploration Vehicle (CEV) Command Module, to characterize its aerodynamics during the high altitude portion of its reentry into the Earth's atmosphere, that is, from free molecular to continuum hypersonic conditions. The focus is on flow conditions similar to those that the Orion Crew Module would experience during a return from the International Space Station. The bulk of the calculations are performed with two direct simulation Monte Carlo (DSMC) codes, and these data are anchored with results from both free molecular and Navier-Stokes calculations. Results for aerodynamic forces and moments are presented that demonstrate their sensitivity to rarefaction, that is, for free molecular to continuum conditions (Knudsen numbers of 111 to 0.0003). Also included are aerodynamic data as a function of angle of attack for different levels of rarefaction and results that demonstrate the aerodynamic sensitivity of the Orion CM to a range of reentry velocities (7.6 to 15 km/s)

VLBI astrometry of PSR J2222-0137: a pulsar distance measured to 0.4% accuracy

The binary pulsar J2222-0137 is an enigmatic system containing a partially recycled millisecond pulsar and a companion of unknown nature. Whilst the low eccentricity of the system favors a white dwarf companion, an unusual double neutron star system is also a possibility, and optical observations will be able to distinguish between these possibilities. In order to allow the absolute luminosity (or upper limit) of the companion object to be properly calibrated, we undertook astrometric observations with the Very Long Baseline Array to constrain the system distance via a measurement of annual geometric parallax. With these observations, we measure the parallax of the J2222-0137 system to be 3.742 +0.013 -0.016 milliarcseconds, yielding a distance of 267.3 +1.2 -0.9 pc, and measure the transverse velocity to be 57.1 +0.3 -0.2 km/s. Fixing these parameters in the pulsar timing model made it possible to obtain a measurement of Shapiro delay and hence the system inclination, which shows that the system is nearly edge-on (sin i = 0.9985 +/- 0.0005). Furthermore, we were able to detect the orbital motion of J2222-0137 in our VLBI observations and measure the longitude of ascending node. The VLBI astrometry yields the most accurate distance obtained for a radio pulsar to date, and is furthermore the most accurate parallax for any radio source obtained at "low" radio frequencies (below ~5 GHz, where the ionosphere dominates the error budget). Using the astrometric results, we show the companion to J2222-0137 will be easily detectable in deep optical observations if it is a white dwarf. Finally, we discuss the implications of this measurement for future ultra-high-precision astrometry, in particular in support of pulsar timing arrays.Comment: 22 pages, 7 figures, accepted for publication in Ap

Snowmobiles in Antarctica

Snowmobiles are the main form of land transportation for field parties in Antarctica. Recently the United States Antarctic program turned almost exclusively to Ski-Doo Alpine 640-ER snowmobiles, the use and maintenance of which require specialized techniques. The first extensive Antarctic field test of these snowmobiles was made during three months of 1977-1978 while engaged in reconnaissance geologic and topographic exploration of the Orville Coast area. Snowmobiles are used to pull large loads of food and gear on two Nansen sledges. When crossing crevasse fields, they are driven remotely by persons on skis. To do this, modifications are made to the stock throttle to enable the engine both to be set at a constant speed and to be shorted out by pulling on a cord that trails behind the snowmobile; steering is by ropes attached to the front ski of the snowmobile. Proper "night" storage is necessary to ensure easiest starting in the morning and to minimize the effects of storms. A routine of trouble-shooting that rapidly isolated and corrected engine problems included first checking spark-plugs or gas-line filters, followed by checking carburetor jet adjustments, drive belt and oil/gas ratio. We found that Ski-Doos are well suited to Antarctica but would be more useful if carburetor fuel filters were replaced by in-line fuel filters and if snowmobiles were equipped with remote throttle controls, tachometers, speedometers, odometers, and a low-gear option

Discovery of Five New Pulsars in Archival Data

Reprocessing of the Parkes Multibeam Pulsar Survey has resulted in the discovery of five previously unknown pulsars and several as-yet-unconfirmed candidates. PSR J0922-52 has a period of 9.68 ms and a DM of 122.4 pc cm^-3. PSR J1147-66 has a period of 3.72 ms and a DM of 133.8 pc cm^-3. PSR J1227-6208 has a period of 34.53 ms, a DM of 362.6 pc cm^-3, is in a 6.7 day binary orbit, and was independently detected in an ongoing high-resolution Parkes survey by Thornton et al. and also in independent processing by Einstein@Home volunteers. PSR J1546-59 has a period of 7.80 ms and a DM of 168.3 pc cm^-3. PSR J1725-3853 is an isolated 4.79-ms pulsar with a DM of 158.2 pc cm^-3. These pulsars were likely missed in earlier processing efforts due to their high DMs and short periods and the large number of candidates that needed to be looked through. These discoveries suggest that further pulsars are awaiting discovery in the multibeam survey data.Comment: 12 pages, 2 figures, 2 tables, accepted to Ap