Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

Introduction This study was designed to investigate the putative anxiolytic-like activity of ultra-low doses of Gelsemium sempervirens (G. sempervirens), produced according to the homeopathic pharmacopeia. Methods Five different centesimal (C) dilutions of G. sempervirens (4C, 5C, 7C, 9C and 30C), the drug buspirone (5 mg/kg) and solvent vehicle were delivered intraperitoneally to groups of ICR-CD1 mice over a period of 9 days. The behavioral effects were assessed in the open-field (OF) and light\u2013dark (LD) tests in blind and randomized fashion. Results Most G. sempervirens dilutions did not affect the total distance traveled in the OF (only the 5C had an almost significant stimulatory effect on this parameter), indicating that the medicine caused no sedation effects or unspecific changes in locomotor activity. In the same test, buspirone induced a slight but statistically significant decrease in locomotion. G. sempervirens showed little stimulatory activity on the time spent and distance traveled in the central zone of the OF, but this effect was not statistically significant. In the LD test, G. sempervirens increased the % time spent in the light compartment, an indicator of anxiolytic-like activity, with a statistically significant effect using the 5C, 9C and 30C dilutions. These effects were comparable to those of buspirone. The number of transitions between the compartments of the LD test markedly increased with G. sempervirens 5C, 9C and 30C dilutions. Conclusion The overall pattern of results provides evidence that G. sempervirens acts on the emotional reactivity of mice, and that its anxiolytic-like effects are apparent, with a non-linear relationship, even at high dilutions

UV-C irradiation as a tool to eradicate algae in caves.

International audienc

Browning Phenomenon of Medieval Stained Glass Windows

International audienc

New anthraquinone drugs and their anticancer activities: Cytotoxicity, DFT, docking and ADMET properties

Amino-anthraquinone derivatives are the most important polycyclic compounds from an occupational health perspective. Among compounds with a cyclic structure, amino anthracene is given high attention because of its carcinogenic potential. The stringent precautions required to handle carcinogens apply to many drugs.Here we synthesize new anthraquinone derivatives with important yield. Characterized with NMR and mass spectroscopy analysis. Then, we evaluate their efficiency against prostate cancer in the PC-3 cell line. From the result, the dialkylated amino-anthraquinone derivatives B and the monoalkylated product with pyrazole exhibited high cytotoxicity potency (IC50 = 0.452 and 0.476 µM for B and D, respectively). From the theoretical study using Molecular Docking, we find the same order of efficiency obtained experimentally by cytotoxicity study. Then, the pharmacokinetic results show that the synthetic drugs used in this study respect the Lipinski, Ghose, Egan, and Veber roles. In addition to this, the density functional theory was used in conjunction with the Gaussian 16 simulation package at the B3LYP/6-311G** level of theory in order to locate the most chemically active compound among the amino-anthraquinone derivatives

Présentation

Flichy Patrice. Présentation. In: Réseaux, volume 12, n°67, 1994. Les jeux vidéo. p. 5

Étude du phénomène de brunissement de vitraux médiévaux

International audienceThe so-called "browning phenomenon" is a particular pathology that concerns Mn-bearing medieval glasses that have been exposed to weath-ering conditions. It leads to the appearance of brown stains that hinders, sometimes completely, light transmission through the stained-glass windows, the artefacts ending up illegible. Visual and chemical character-ization of the pathology was performed as to optimize the diagnosis of darkened glasses. The leached layer and the Mn-inclusions were studied to a micrometric scale in order to identify the oxidation state and the atomic environment of manganese ions. In parallel, a review of the exist-ing treatments was performed in order to validate different existing proto-cols previously applied. New tests were conducted in order to provide a better understanding of the possible effects induced on historical glasses by the application of reducing agents.Sous l'effet de l'environnement, certains verres médiévaux contenant du manganèse développent une pathologie communément appelée « phéno-mène de brunissement » qui se révèle par l'apparition de taches brunes. Il en résulte une perte importante et parfois totale de la transparence du vitrail, ce qui rend l'oeuvre illisible. Une caractérisation visuelle et chimique de vingt-deux verres historiques a été réalisée afin d'optimiser les critères permettant le diagnostic de cette pathologie. Les phases d'altération ont également été étudiées à l'échelle micrométrique afin de déterminer le degré d'oxydation et l'environnement du manganèse. En parallèle, une synthèse des traitements existants a été menée et six d'entre eux ont été testés afin de valider les protocoles mis en place lors de précédentes études et de mieux comprendre les différents effets induits par l'utilisation d'agents réducteurs