Permanence et changements du métier de formateur, quel avenir pour la formation infirmière ?

Les formateurs sont des cadres de santé exerçant à l'hôpital ou en institut de formation. On a décrit l'émergence et l'évolution de cette formation, (associations, syndicats, définitions, programmes, institutions) et étudié les caractéristiques sociales et les trajectoires des formateurs. La formation infirmière s'inscrit dans une permanence de tradition hospitalière de soumission à l'autorité médicale et administrative. Elle subit des changements suffisamment opérants et fréquents pour agir sur les modalités de recrutement des étudiants et les motivations à entamer la carrière de formateur ou d'infirmier. Devenir formateur en 2003, consiste à quitter un métier d'expérience pratique pour devenir un théoricien du soin et " faire face " à la pénurie infirmière en gérant les contraintes d'un métier en crise.ST DENIS-BU PARIS8 (930662101) / SudocSudocFranceF

The multifunctional protein GC1q-R interacts specifically with the i3 loop arginine cluster of the vasopressin V2 receptor

International audienceIn this study, we identified the multifunctional protein GC1q-R as a novel vasopressin V(2) receptor (V(2)R) interacting protein. For this purpose, we have developed a proteomic approach combining pull-down assays using a cyclic peptide mimicking the third intracellular loop of V(2)R as a bait and mass spectrometry analyses of proteins isolated from either rat or human kidney tissues or the HEK 293 cell line. Co-immunoprecipitation of GC1q-R with the c-Myc-tagged h-V(2)R expressed in a HEK cell line confirmed the existence of a specific interaction between GC1q-R and the V(2) receptor. Then, construction of a mutant receptor in i3 loop allowed us to identify the i3 loop arginine cluster of the vasopressin V(2) receptor as the interacting determinant for GC1q-R interaction. Using purified receptor as a bait and recombinant (74-282) GC1q-R, we demonstrated a direct and specific interaction between these two proteins via the arginine cluster

Mettre en scène le conte

Après « Mettre en scène l’événement » à partir du cas de la pièce 11 septembre 2001 de Michel Vinaver, ce deuxième hors-série aspire à ouvrir un espace de réflexion autour du conte en scène, à partir de l’étude de la réécriture théâtrale de Cendrillon par l’auteur-metteur en scène Joël Pommerat (2011). Le choix d’inscrire Cendrillon, troisième conte réécrit par Pommerat, au programme du Baccalauréat option théâtre (2013-2015) nous a paru symptomatique d’un changement de regard porté sur les contes et leurs usages. Si, d’un point de vue méthodologique, on fait l’hypothèse que Cendrillon de Pommerat est un exemple représentatif d’une tendance plus vaste, que nous apprend cette œuvre des nouvelles alliances entre contes et arts de la scène contemporaine ? Qu’en est-il des nouveaux usages du conte en scène, et plus particulièrement de Cendrillon dans le cadre d’une formation au théâtre pour futurs bacheliers ? Ce dossier se développera au fil de publications invitées « en continu » à le compléter dans les mois à venir

Leukotriene BLT2 Receptor Monomers Activate the Gi2 GTP-binding Protein More Efficiently than Dimers*

Accumulating evidence indicates that G protein-coupled receptors can assemble as dimers/oligomers but the role of this phenomenon in G protein coupling and signaling is not yet clear. We have used the purified leukotriene B4 receptor BLT2 as a model to investigate the capacity of receptor monomers and dimers to activate the adenylyl cyclase inhibitory Gi2 protein. For this, we overexpressed the recombinant receptor as inclusion bodies in the Escherichia coli prokaryotic system, using a human α5 integrin as a fusion partner. This strategy allowed the BLT2 as well as several other G protein-coupled receptors from different families to be produced and purified in large amounts. The BLT2 receptor was then successfully refolded to its native state, as measured by high-affinity LTB4 binding in the presence of the purified G protein Gαi2. The receptor dimer, in which the two protomers displayed a well defined parallel orientation as assessed by fluorescence resonance energy transfer, was then separated from the monomer. Using two methods of receptor-catalyzed guanosine 5′-3-O-(thio)triphosphate binding assay, we clearly demonstrated that monomeric BLT2 stimulates the purified Gαi2β1γ2 protein more efficiently than the dimer. These data suggest that assembly of two BLT2 protomers into a dimer results in the reduced ability to signal

Predictive usefulness of RT-PCR testing in different patterns of Covid-19 symptomatology: analysis of a French cohort of 12,810 outpatients

International audienceReverse transcriptase polymerase chain reaction (RT-PCR) is a key tool to diagnose Covid-19. Yet it may not be the most efficient test in all patients. In this paper, we develop a clinical strategy for prescribing RT-PCR to patients based on data from COVIDOM, a French cohort of 54,000 patients with clinically suspected Covid-19, including 12,810 patients tested by RT-PCR. We use a machine-learning algorithm (decision tree) in order to predict RT-PCR results based on the clinical presentation. We show that symptoms alone are sufficient to predict RT-PCR outcome with a mean average precision of 86%. We identify combinations of symptoms that are predictive of RT-PCR positivity (90% for anosmia/ageusia) or negativity (only 30% of RT-PCR+\,for a subgroup with cardiopulmonary symptoms): in both cases, RT-PCR provides little added diagnostic value. We propose a prescribing strategy based on clinical presentation that can improve the global efficiency of RT-PCR testing