Exploring the Role of Vitamin D. Comments on Fleury et al. Sun Exposure and Its Effects on Human Health: mechanisms through Which Sun Exposure Could Reduce the Risk of Developing Obesity and Cardiometabolic Dysfunction

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Why vitamin D clinical trials should be based on 25-hydroxyvitamin D concentrations

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Preschool children's motor development and perceived competence

This study compared the effects of two conditions on the motor development of preschool children and investigated the relationship between preschool children's motor development and perceived competence. Specifically, it provided information for discerning: a) the relative effects of a sensory-motor condition and an unstructured activities condition on the motor development of preschool children immediately following the 20-week intervention; and b) whether perceived competence was related to motor development in the preschool children following termination of the treatment. Additionally, a teacher survey which addressed the teacher-consultant relationship was developed and piloted within the context of the study for use in future research. Subjects were 31 children enrolled in two preschool programs including: a) the curriculum group (N=16), and b) the non-curriculum group (N=l5). The Peabody Motor Developmental Scales and the Pictorial Scale of Perceived Competence and Social Acceptance were used to assess the children's motor development and perceived competence, respectively. All subjects were tested prior to the 20- week intervention period and immediately following the intervention. A series of 2 (group) X 2 (testing time) repeated measures analyses of variance were used to analyze the impact of the two conditions. Results revealed that the motor development of subjects in both groups changed significantly over time; however, there were no differences between groups. Product-moment correlations and linear regression analyses were used to assess the relationship between preschool children's motor development and perceived competence. Results revealed that perceived competence relative to motor development did not change over time; however a reciprocal relationship between motor development and perceived competence in preschool children was found. The piloted teacher survey showed potential for evaluation of service delivery models and as a tool for teacher-consultant communication in future studies

Comparing the Evidence from Observational Studies and Randomized Controlled Trials for Nonskeletal Health Effects of Vitamin D

Although observational studies of health outcomes generally suggest beneficial effects with, or following, higher serum 25-hydroxyvitamin D [25(OH)D] concentrations, randomized controlled trials (RCTs) have generally not supported those findings. Here we review results from observational studies and RCTs regarding how vitamin D status affects several nonskeletal health outcomes, including Alzheimer\u27s disease and dementia, autoimmune diseases, cancers, cardiovascular disease, COVID-19, major depressive disorder, type 2 diabetes, arterial hypertension, all-cause mortality, respiratory tract infections, and pregnancy outcomes. We also consider relevant findings from ecological, Mendelian randomization, and mechanistic studies. Although clear discrepancies exist between findings of observational studies and RCTs on vitamin D and human health benefits these findings should be interpreted cautiously. Bias and confounding are seen in observational studies and vitamin D RCTs have several limitations, largely due to being designed like RCTs of therapeutic drugs, thereby neglecting vitamin D\u27s being a nutrient with a unique metabolism that requires specific consideration in trial design. Thus, RCTs of vitamin D can fail for several reasons: few participants\u27 having low baseline 25(OH)D concentrations, relatively small vitamin D doses, participants\u27 having other sources of vitamin D, and results being analyzed without consideration of achieved 25(OH)D concentrations. Vitamin D status and its relevance for health outcomes can usefully be examined using Hill\u27s criteria for causality in a biological system from results of observational and other types of studies before further RCTs are considered and those findings would be useful in developing medical and public health policy, as they were for nonsmoking policies. A promising approach for future RCT design is adjustable vitamin D supplementation based on interval serum 25(OH)D concentrations to achieve target 25(OH)D levels suggested by findings from observational studies

Differential binding regulation of microtubule-associated proteins MAP1A, MAP1B, and MAP2 by tubulin polyglutamylation.

The major neuronal post-translational modification of tubulin, polyglutamylation, can act as a molecular potentiometer to modulate microtubule-associated proteins (MAPs) binding as a function of the polyglutamyl chain length. The relative affinity of Tau, MAP2, and kinesin has been shown to be optimal for tubulin modified by approximately 3 glutamyl units. Using blot overlay assays, we have tested the ability of polyglutamylation to modulate the interaction of two other structural MAPs, MAP1A and MAP1B, with tubulin. MAP1A and MAP2 display distinct behavior in terms of tubulin binding; they do not compete with each other, even when the polyglutamyl chains of tubulin are removed, indicating that they have distinct binding sites on tubulin. Binding of MAP1A and MAP1B to tubulin is also controlled by polyglutamylation and, although the modulation of MAP1B binding resembles that of MAP2, we found that polyglutamylation can exert a different mode of regulation toward MAP1A. Interestingly, although the affinity of the other MAPs tested so far decreases sharply for tubulins carrying long polyglutamyl chains, the affinity of MAP1A for these tubulins is maintained at a significant level. This differential regulation exerted by polyglutamylation toward different MAPs might facilitate their selective recruitment into distinct microtubule populations, hence modulating their functional properties

In Vivo Airway Surface Liquid Cl− Analysis with Solid-State Electrodes

The pathogenesis of cystic fibrosis (CF) airways disease remains controversial. Hypotheses that link mutations in CFTR and defects in ion transport to CF lung disease predict that alterations in airway surface liquid (ASL) isotonic volume, or ion composition, are critically important. ASL [Cl−] is pivotal in discriminating between these hypotheses, but there is no consensus on this value given the difficulty in measuring [Cl−] in the “thin” ASL (∼30 μm) in vivo. Consequently, a miniaturized solid-state electrode with a shallow depth of immersion was constructed to measure ASL [Cl−] in vivo. In initial experiments, the electrode measured [Cl−] in physiologic salt solutions, small volume (7.6 μl) test solutions, and in in vitro cell culture models, with ≥93% accuracy. Based on discrepancies in reported values and/or absence of data, ASL Cl− measurements were made in the following airway regions and species. First, ASL [Cl−] was measured in normal human nasal cavity and averaged 117.3 ± 11.2 mM (n = 6). Second, ASL [Cl−] measured in large airway (tracheobronchial) regions were as follows: rabbit trachea and bronchus = 114.3 ± 1.8 mM; (n = 6) and 126.9 ± 1.7 mM; (n = 3), respectively; mouse trachea = 112.8 ± 4.2 mM (n = 13); and monkey bronchus = 112.3 ± 10.9 mM (n = 3). Third, Cl− measurements were made in small (1–2 mm) diameter airways of the rabbit (108.3 ± 7.1 mM, n = 5) and monkey (128.5 ± 6.8 mM, n = 3). The measured [Cl−], in excess of 100 mM throughout all airway regions tested in multiple species, is consistent with the isotonic volume hypothesis to describe ASL physiology

Salvage logging effects on regulating and supporting ecosystem services. A systematic map

Wildfires, insect outbreaks, and windstorms are increasingly common forest disturbances.Post-disturbance management often involves salvage logging, i.e. the felling and removal of the affected trees. However, this practice may represent an additional disturbance witheffects on ecosystem processes and services. We developed a systematic map to provide an overview of the primary studies on this topic, and created a database with information on the characteristics of the retrieved publications, including information on stands, disturbance, intervention, measured outcomes, and study design. Of 4341 retrieved publications, 90 were retained in the systematic map. These publications represented 49 studies, predominantly from North America and Europe. Salvage logging after wildfire was addressed more frequently than after insect outbreaks or windstorms. Most studies addressed logging after a single disturbance event, and replication of salvaged stands rarely exceeded 10. The most frequent response variables were tree regeneration, ground cover, and deadwood characteristics.Junta de AndalucíaREMEDINA