Atmospheric cycles of nitrogen oxides and ammonia

The atmospheric cycles of nitrogenous trace compounds for the Northern and Southern Hemispheres are discussed. Source strengths and destruction rates for the nitrogen oxides: NO, NO2 and HNO3 -(NOX) and ammonia (NH3) are given as a function of latitude over continents and oceans. The global amounts of NOX-N and NH3-N produced annually in the period 1950 to 1975 (34 + 5 x one trillion g NOx-N/yr and 29 + or - 6 x one trillion g NH3-N/yr) are much less than previously assumed. Globally, natural and anthropogenic emissions are of similar magnitude. The NOx emission from anthropogenic sources is 1.5 times that from natural processes in the Northern Hemisphere, whereas in the Southern Hemisphere, it is a factor of 3 or 4 less. More than 80% of atmospheric ammonia seems to be derived from excrements of domestic animals, mostly by bulk deposition: 24 + or - 9 x one trillion g NO3 -N/yr and 21 + or - 9 x one trillion g NH4+-N/yr. Another fraction may be removed by absorption on vegetation and soils

Improving Lifelong Learning by Fostering Students' Learning Strategies at University

The foundation of how students usually learn is laid early in their academic lives. However, many or even most students do not primarily rely on those learning strategies that are most favorable from a scientific point of view. To change students' learning behavior when they start their university education, we developed a computer-based adaptive learning environment to train favorable learning strategies and change students' habits using them. This learning environment pursues three main goals: acquiring declarative and conditional knowledge about learning strategies, consolidating that knowledge, and applying these learning strategies in practice. In this report, we describe four experimental studies conducted to optimize this learning environment (n = 336). With those studies, we improved the learning environment with respect to how motivating it is, investigated an efficient way to consolidate knowledge, and explored how to facilitate the formation of effective implementation intentions for applying learning strategies and changing learning habits. Our strategy-training module is implemented in the curriculum for freshman students at the Department of Psychology, University of Freiburg (Germany). Around 120 students take part in our program every year. An open version of this training intervention is freely available to everyone

Frequency-dependent (ac) Conduction in Disordered Composites: a Percolative Study

In a recent paper [Phys. Rev. B{\bf57}, 3375 (1998)], we examined in detail the nonlinear (electrical) dc response of a random resistor cum tunneling bond network ($RRTN$, introduced by us elsewhere to explain nonlinear response of metal-insulator type mixtures). In this work which is a sequel to that paper, we consider the ac response of the $RRTN$-based correlated $RC$ ($CRC$) model. Numerical solutions of the Kirchoff's laws for the $CRC$ model give a power-law exponent (= 0.7 near $p = p_c$) of the modulus of the complex ac conductance at moderately low frequencies, in conformity with experiments on various types of disordered systems. But, at very low frequencies, it gives a simple quadratic or linear dependence on the frequency depending upon whether the system is percolating or not. We do also discuss the effective medium approximation ($EMA$) of our $CRC$ and the traditional random $RC$ network model, and discuss their comparative successes and shortcomings.Comment: Revised and reduced version with 17 LaTeX pages plus 8 JPEG figure

Percolation Systems away from the Critical Point

This article reviews some effects of disorder in percolation systems even away from the critical density p_c. For densities below p_c, the statistics of large clusters defines the animals problem. Its relation to the directed animals problem and the Lee-Yang edge singularity problem is described. Rare compact clusters give rise to Griffiths singuraties in the free energy of diluted ferromagnets, and lead to a very slow relaxation of magnetization. In biassed diffusion on percolation clusters, trapping in dead-end branches leads to asymptotic drift velocity becoming zero for strong bias, and very slow relaxation of velocity near the critical bias field.Comment: Minor typos fixed. Submitted to Praman

Stress development and adhesion in hydrogenated nano-columnar Pd and Pd/Ti ultra-thin films

Abstract. Stress development upon hydrogenation of about 100 nm thick palladium layers on thermally oxidized silicon wafers with and without an intermediate Ti layer is studied. Stress developed is investigated by in-situ XRD in H 2 /N 2 (hydrogenation) and N 2 (dehydrogenation) gas at RT. The method adopted to measure residual stress involved specimen omega-(ω) and psi-(ψ) tilting, on two different diffractometer geometries (focusing and parallel). For the stress analysis, the presence of intrinsic elastic anisotropy of the film is considered. Upon hydrogenation α-Pd transformation to β-PdH 0.6 occurs and because of the constrained in-plane expansion a large compressive stress develops. Scanning electron microscopy shows that films with a Ti intermediate layer adhere better to the substrate upon hydrogen cycling, whereas, pure Pd film start cracking and buckling

Plant responses to fluctuating UV environments

Peer reviewe

Rapid intraoperative insulin assay: a novel method to differentiate insulinoma from nesidioblastosis in the pediatric patient

Introduction: Hyperinsulinism is the most common cause of recurrent and persistent hypoglycemia in infancy and childhood. Causes can include nesidioblastosis, pancreatic islet cell tumors such as insulinoma, and associations with multiple endocrine neoplasia syndromes. Although new, improved imaging techniques have allowed for more precise preoperative localization of insulinomas, the differentiation of nesidioblastosis and insulinoma, particularly in children, can be challenging. To improve intraoperative localization and confirmation of successful resection of insulinoma, a novel hormonal assay, the rapid intraoperative insulin assay, is reported for the first time in a pediatric patient. This intraoperative radioimmunoassay for insulin yields results within several minutes and confirms complete resection of insulinoma. Case description: We present a case of pancreatic insulinoma in a child with symptoms of severe hypoglycemia, causing seizures. The insulinoma was enucleated laparoscopically, and rapid intra-operative insulin assay used to determine the success of the procedure. Discussion and evaluation: This rapid intra-operative test provides a valuable adjunct for determining complete excision in complicated cases of recurrent or questionable insulinoma. Although not a common problem, for pediatric patients in whom the diagnosis is not clear, this test may provide a novel approach to confirming disease. Conclusion: We propose the use of this assay in facilitating intra-operative resection and confirmation of complete excision in pediatric patients. This population may especially benefit from this novel assay to confirm complete resection and to differentiate multiple etiologies of hyperinsulinism

Identification of protein biomarkers for prediction of response to platinum-based treatment regimens in patients with non-small cell lung cancer

The majority of patients with resected stage II-IIIA non-small cell lung cancer (NSCLC) are treated with platinum-based adjuvant chemotherapy (ACT) in a one-size-fits-all approach. However, a significant number of patients do not derive clinical benefit, and no predictive patient selection biomarker is currently available. Using mass spectrometry-based proteomics, we have profiled tumour resection material of 2 independent, multi-centre cohorts of in total 67 patients with NSCLC who underwent ACT. Unsupervised cluster analysis of both cohorts revealed a poor response/survival sub-cluster composed of ~ 25% of the patients, that displayed a strong epithelial-mesenchymal transition signature and stromal phenotype. Beyond this stromal sub-population, we identified and validated platinum response prediction biomarker candidates involved in pathways relevant to the mechanism of action of platinum drugs, such as DNA damage repair, as well as less anticipated processes such as those related to the regulation of actin cytoskeleton. Integration with pre-clinical proteomics data supported a role for several of these candidate proteins in platinum response prediction. Validation of one of the candidates (HMGB1) in a third independent patient cohort using immunohistochemistry highlights the potential of translating these proteomics results to clinical practice.</p

mRNA Display Selection of an Optimized MDM2-Binding Peptide That Potently Inhibits MDM2-p53 Interaction

p53 is a tumor suppressor protein that prevents tumorigenesis through cell cycle arrest or apoptosis of cells in response to cellular stress such as DNA damage. Because the oncoprotein MDM2 interacts with p53 and inhibits its activity, MDM2-p53 interaction has been a major target for the development of anticancer drugs. While previous studies have used phage display to identify peptides (such as DI) that inhibit the MDM2-p53 interaction, these peptides were not sufficiently optimized because the size of the phage-displayed random peptide libraries did not cover all of the possible sequences. In this study, we performed selection of MDM2-binding peptides from large random peptide libraries in two stages using mRNA display. We identified an optimal peptide named MIP that inhibited the MDM2-p53 and MDMX-p53 interactions 29- and 13-fold more effectively than DI, respectively. Expression of MIP fused to the thioredoxin scaffold protein in living cells by adenovirus caused stabilization of p53 through its interaction with MDM2, resulting in activation of the p53 pathway. Furthermore, expression of MIP also inhibited tumor cell proliferation in a p53-dependent manner more potently than DI. These results show that two-stage, mRNA-displayed peptide selection is useful for the rapid identification of potent peptides that target oncoproteins