Assessment of Customer Expectation and Perception of Service Quality Delivery in Ghana Commercial Bank

The study is to determine customers\u27 expectations and perception of quality of servicedelivery of Ghana Commercial Banks Limited. The study also aims at determining thegap between customers\u27 expectation and perception of service quality delivery(customer satisfaction /dissatisfaction) of the banks. To achieve the main aim of thestudy, the following specific objectives have been set out; determine customers\u27expectations of the quality of service delivery of GCB, measure customers\u27 perceptionof the quality of service delivery of GCB, determine the gap (customer satisfaction ordissatisfaction) between customers\u27 perception and expectation of service qualitydelivery of the bank

Oxygen mapping of melanoma spheroids using small molecule platinum probe and phosphorescence lifetime imaging microscopy

Solid tumours display varied oxygen levels and this characteristic can be exploited to develop new diagnostic tools to determine and exploit these variations. Oxygen is an efficient quencher of emission of many phosphorescent compounds, thus oxygen concentration could in many cases be derived directly from relative emission intensity and lifetime. In this study, we extend our previous work on phosphorescent, low molecular weight platinum(II) complex as an oxygen sensing probe to study the variation in oxygen concentration in a viable multicellular 3D human tumour model. The data shows one of the first examples of non-invasive, real-time oxygen mapping across a melanoma tumour spheroid using one-photon phosphorescence lifetime imaging microscopy (PLIM) and a small molecule oxygen sensitive probe. These measurements were quantitative and enabled real time oxygen mapping with high spatial resolution. This combination presents as a valuable tool for optical detection of both physiological and pathological oxygen levels in a live tissue mass and we suggest has the potential for broader clinical application

Environmental performance indicators for assessing sustainability of projects in the Ghanaian construction industry

PURPOSE: Recent reports based on the sustainable development goals (SDGs) have revealed that no country is in line with achieving the targets of the 2030 Agenda for sustainable development, with the slowest progress being witnessed mainly on goals that are focused on the environment. This study examines environmental performance indicators for assessing the sustainability of building projects. DESIGN/METHODOLOGY/APPROACH: The study uses an explanatory sequential design with an initial quantitative instrument phase, followed by a qualitative data collection phase. An extensive critical comparative review of the literature resulted in the identification of ten environmental sustainability indicators. One hundred and sixty-seven questionnaire responses based upon these indicators from the Ghanaian construction industry were received. Data were coded with SPSS v22, analysed descriptively, and via inferential analysis. These data were then validated through semi-structured interviews with six interviewees who are fellows of their respective professional bodies, a senior academic (professor in construction project delivery) and a government official. Data obtained from the semi-structured validation interviews were analysed through the side-by-side comparison of the qualitative data with the quantitative data. FINDINGS: The findings from the study suggest that all the indicators were important in assessing building projects' environmental sustainability across the entire life cycle. Key among the identified indicators is the effects of the project on “water quality, air quality, energy use and conservation, and environmental compliance and management”. The interviewees further agreed to and confirmed the importance of these identified indicators for assessing the environmental sustainability of building projects in Ghana. ORIGINALITY/VALUE: Compared to existing studies, this study adopts the exploratory sequential design to identify and examine the critical indicators in assessing the environmental sustainability across the entire lifecycle of building projects in a typical developing country setting, i.e. Ghana. It reveals areas of prime concern in the drive to place the local construction industry on a trajectory towards achieving environmental sustainability

Time-resolved fluorescence imaging reveals differential interactions of N-glycan processing enzymes across the Golgi stack in planta

N-glycan processing is one of the most important cellular protein modifications in plants and as such is essential for plant development and defense mechanisms. The accuracy of Golgi-located processing steps is governed by the strict intra-Golgi localization of sequentially acting glycosidases and glycosyltransferases. Their differential distribution goes hand in hand with the compartmentalization of the Golgi stack into cis-, medial and trans-cisternae, which separate early from late processing steps. The mechanisms that direct differential enzyme concentration are still unknown, but formation of multi-enzyme complexes is considered a feasible Golgi protein localization strategy. In this study we used two-photon (2P)-excitation Förster resonance energy transfer (FRET)-fluorescence lifetime imaging microscopy (FLIM) to determine the interaction of N-glycan processing enzymes with differential intra-Golgi locations. Following the coexpression of fluorescent protein-tagged N-terminal Golgi targeting sequences (cytoplasmic-transmembrane-stem region, designated CTS) of enzyme pairs in leaves of tobacco (Nicotiana tabacum or Nicotiana benthamiana), we observed that all tested cis- and medial-Golgi enzymes, namely MNS1, GnTI, GMII and XylT, form homo- and heterodimers, whereas among the late-acting enzymes GALT1, FUT13 and ST (a non-plant Golgi marker) only GALT1 and GMII were found to form a heterodimer. Furthermore, the efficiency of energy transfer indicating the formation of interactions decreased considerably in a cis-to-trans fashion. The comparative 2P-FRET-FLIM analysis of several full-length cis- and medial-Golgi enzymes and their respective catalytic domain-deleted CTS clones further suggested that the formation of protein-protein interactions can occur through their N-terminal CTS region

The need to address fragmentation and silos in mortality information systems: the case of Ghana and Peru

Objectives: We aimed to understand the information architecture and degree of integration of mortality surveillance systems in Ghana and Peru. Methods: We conducted a cross-sectional study using a combination of document review and unstructured interviews to describe and analyse the sub-systems collecting mortality data. Results: We identified 18 and 16 information subsystems with independent databases capturing death events in Peru and Ghana respectively. The mortality information architecture was highly fragmented with a multiplicity of unconnected data silos and with formal and informal data collection systems. Conclusion: Reliable and timely information about who dies where and from what underlying cause is essential to reporting progress on Sustainable Development Goals, ensuring policies are responding to population health dynamics, and understanding the impact of threats and events like the COVID-19 pandemic. Integrating systems hosted in different parts of government remains a challenge for countries and limits the ability of statistics systems to produce accurate and timely information. Our study exposes multiple opportunities to improve the design of mortality surveillance systems by integrating existing subsystems currently operating in silos

A C-terminal amphipathic helix is necessary for the in vivo tubule-shaping function of a plant reticulon.

Reticulons (RTNs) are a class of endoplasmic reticulum (ER) membrane proteins that are capable of maintaining high membrane curvature, thus helping shape the ER membrane into tubules. The mechanism of action of RTNs is hypothesized to be a combination of wedging, resulting from the transmembrane topology of their conserved reticulon homology domain, and scaffolding, arising from the ability of RTNs to form low-mobility homo-oligomers within the membrane. We studied the plant RTN isoform RTN13, which has previously been shown to locate to ER tubules and the edges of ER cisternae and to induce constrictions in ER tubules when overexpressed, and identified a region in the C terminus containing a putative amphipathic helix (APH). Here we show that deletion of this region or disruption of the hydrophobic face of the predicted helix abolishes the ability of RTN13 to induce constrictions of ER tubules in vivo. These mutants, however, still retain their ability to interact and form low-mobility oligomers in the ER membrane. Hence, our evidence indicates that the conserved APH is a key structural feature for RTN13 function in vivo, and we propose that RTN, like other membrane morphogens, rely on APHs for their function

Drivers for Design for Deconstruction (DfD) Implementation Among Design Professionals

Purpose- The Design for Deconstruction (DfD) technique, a contemporaneous solution to demolition by optimizing disassembly activities to enable reuse, has recently emerged with several promises to promote the circular economy. However, little attention has been given to its implementation among design professionals, especially in the Global South. Therefore, this study explores the drivers for DfD implementation among design professionals in the Ghanaian construction industry (GCI). Design/methodology/approach- The study adopted a mixed research approach (explanatory sequential design) with an initial quantitative instrument phase, followed by a qualitative data collection phase. Data were analyzed using mean, standard deviation, One-Sample t-Test, and Normalization Value (NV) test after a review of pertinent literature. These data were then validated through semi-structured interviews with ten design professionals with in-depth knowledge of DfD. Findings- The findings revealed that although all eleven drivers are important, the eight key drivers for the DfD implementation were identified as, in order of importance, Availability of computer software applications regarding DfD , Inclusion of DfD in the formal education of design professionals , Increasing public awareness of the concept of DfD , Organizing workshops/seminars for design professionals on the concept of DfD , Availability of DfD training , Regulation regarding DfD , Industry guidance regarding DfD and Establishing a market for salvaged construction components . Originality/value- This study\u27s findings provide insights into an under-investigated topic in Ghana and offer new and additional information and insights into the current state-of-the-art on the factors that drive DfD implementation

Exploring the barriers to mental health help-seeking among African migrants in Australia: A qualitative study

Background: The health of African migrants in Australia is a largely under-researched topic despite the steadily increasing size of the population and its uniqueness. In particular, few studies have explored the mental health of African migrants in Australia or their utilization of mental health services. Aims: This study explored the barriers to mental health help-seeking among first-generation African migrants in Australia. Methods: In this qualitative study conducted using the hermeneutical phenomenological research approach, the purposive sampling method was used to recruit participants. In-depth interviews with participants were undertaken online via Zoom, Teams, and WhatsApp calls. Interviews were recorded and transcribed verbatim, utilizing a thematic analysis as the primary data analysis method. Results: African migrants were more inclined to seek support for mental health conditions from religious figures such as priests instead of seeking professional help. Religion and poor knowledge about mental illness were highlighted as barriers to mental health help-seeking. Participants also considered cultural beliefs, fear of stigma as well as the high cost of healthcare in Australia as significant barriers to mental health help-seeking among African migrants. Conclusions: Findings reinforce the critical need for culturally competent mental health services tailored to the beliefs, values, religion, and experiences of African and other migrant communities in Australia. Given the strong attachment of many African migrants in Australia to their cultural and religious beliefs, such services are essential for practical support and intervention

Influence of Topology and Brønsted Acid Site Presence on Methanol Diffusion in Zeolites Beta and MFI

Detailed insight into molecular diffusion in zeolite frameworks is crucial for the analysis of the factors governing their catalytic performance in methanol-to-hydrocarbons (MTH) reactions. In this work, we present a molecular dynamics study of the diffusion of methanol in all-silica and acidic zeolite MFI and Beta frameworks over the range of temperatures 373–473 K. Owing to the difference in pore dimensions, methanol diffusion is more hindered in H-MFI, with diffusion coefficients that do not exceed 10×10−10 m2s−1. In comparison, H-Beta shows diffusivities that are one to two orders of magnitude larger. Consequently, the activation energy of translational diffusion can reach 16 kJ·mol−1 in H-MFI, depending on the molecular loading, against a value for H-Beta that remains between 6 and 8 kJ·mol−1. The analysis of the radial distribution functions and the residence time at the Brønsted acid sites shows a greater probability for methylation of the framework in the MFI structure compared to zeolite Beta, with the latter displaying a higher prevalence for methanol clustering. These results contribute to the understanding of the differences in catalytic performance of zeolites with varying micropore dimensions in MTH reactions

Fluorescence and phosphorescence lifetime imaging reveals a significant cell nuclear viscosity and refractive index changes upon DNA damage

Cytoplasmic viscosity is a crucial parameter in determining rates of diffusion-limited reactions. Changes in viscosity are associated with several diseases, whilst nuclear viscosity determines gene integrity, regulation and expression. Yet how drugs including DNA-damaging agents affect viscosity is unknown. We demonstrate the use of a platinum complex, Pt[L]Cl, that localizes efficiently mostly in the nucleus as a probe for nuclear viscosity. The phosphorescence lifetime of Pt[L]Cl is sensitive to viscosity and provides an excellent tool to investigate the impact of DNA damage. We show using Fluorescence Lifetime Imaging (FLIM) that the lifetime of both green and red fluorescent proteins (FP) are also sensitive to changes in cellular viscosity and refractive index. However, Pt[L]Cl proved to be a more sensitive viscosity probe, by virtue of microsecond phosphorescence lifetime versus nanosecond fluorescence lifetime of FP, hence greater sensitivity to bimolecular reactions. DNA damage was inflicted by either a two-photon excitation, one-photon excitation microbeam and X-rays. DNA damage of live cells causes significant increase in the lifetime of either Pt[L]Cl (HeLa cells, 12.5–14.1 µs) or intracellularly expressed mCherry (HEK293 cells, 1.54–1.67 ns), but a decrease in fluorescence lifetime of GFP from 2.65 to 2.29 ns (in V15B cells). These values represent a viscosity change from 8.59 to 20.56 cP as well as significant changes in the refractive index (RI), according to independent calibration. Interestingly DNA damage localized to a submicron region following a laser microbeam induction showed a whole cell viscosity change, with those in the nucleus being greater than the cytoplasm. We also found evidence of a by-stander effect, whereby adjacent un-irradiated cells also showed nuclear viscosity change. Finally, an increase in viscosity following DNA damage was also observed in bacterial cells with an over-expressed mNeonGreen FP, evidenced by the change in its lifetime from 2.8 to 2.4 ns