39 research outputs found

    Comparative Models of Hydrocarbon Emissions for a Diesel Engine Operating at Constant Loads and Speeds

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    Linear multiple regression (LMR) and nonlinear polynomial network (NPN) models were developed from data collected from ISO 8178‐4 (1996) test cycle B‐type tests (ISO) and an expanded set of tests (EXP) to predict hydrocarbon (HC) emissions from a diesel engine. LMR using the ISO training data (R2 = 0.94) resulted in overfitting of the model as applied to the evaluation data (R2 = 0.49). LMR based on the expanded data (R2 = 0.68) was a better LMR model when applied to the evaluation data (R2 = 0.64). NPN using the expanded training data (R2 = 0.99) resulted in the best model when applied to the evaluation data (R2 = 0.98) and is preferred for predicting HC when the larger set of test mode data are available. NPN using the ISO training data (R2 = 0.99) resulted in a satisfactory fit for the evaluation data (R2 = 0.91), although with a higher average absolute error (0.52 vs. 0.42 g/kWh) than NPN using the EXP training data. This model was also considered suitable for predicting HC. Results of this initial study suggest that data could be collected during ISO 8178‐4 emission tests and modeled with NPN to predict HC emissions for a diesel engine operating at various constant speeds and loads

    Modeling NO Emissions of an Off-road Diesel Engine Based on Emission Tests

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    Emissions values determined by the ISO 8178 emission certification tests do not necessarily represent emissions of a tractor in operation (Hansson et al., 2001). Rather than using ISO 8178 tests solely for certification, data collected during the tests may be suitable for predicting nitrogen oxide (NOx) emissions of an engine operating at constant loads and speeds. Linear multiple regression (LMR) and nonlinear polynomial network (NPN) models were developed with data collected from ISO 8178-4 (1996) test cycle B-type tests (ISO) and an expanded set of tests (EXP) to predict NOx emissions from a diesel engine. LMR using the ISO training data (R2 = 0.94) resulted in over-training of the model, as applied to the evaluation data (R2 = 0.51). LMR based on the expanded data (R2 = 0.60) was a better LMR model, when applied to the evaluation data (R2 = 0.73). NPN using the ISO training data (R2 = 0.99) resulted in a considerable improvement over the LMR models for the evaluation data (R2 = 0.81). NPN using the EXP training data (R2 = 0.96) resulted in the best model when applied to the evaluation data (R2 = 0.95). When applied to the evaluation data, the mean absolute error of the NPN EXP based model was significantly less than from the NPN ISO based model. The NPN model based on EXP data is recommended for predicting NOx. Data could be collected during ISO 8178-4 emission tests that included additional test modes and modeled with NPN to predict NOx emissions for a diesel engine operating at various constant speeds and loads

    Economies of Scale: A Survey of the Empirical Literature

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    Use of Genomic DNA as an Indirect Reference for Identifying Gender-Associated Transcripts in Morphologically Identical, but Chromosomally Distinct, Schistosoma mansoni Cercariae

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    BACKGROUND: The use of DNA microarray technology to study global Schistosoma gene expression has led to the rapid identification of novel biological processes, pathways or associations. Implementation of standardized DNA microarray protocols across laboratories would assist maximal interpretation of generated datasets and extend productive application of this technology. METHODOLOGY/PRINCIPAL FINDINGS: Utilizing a new Schistosoma mansoni oligonucleotide DNA microarray composed of 37,632 elements, we show that schistosome genomic DNA (gDNA) hybridizes with less variation compared to complex mixed pools of S. mansoni cDNA material (R = 0.993 for gDNA compared to R = 0.956 for cDNA during ‘self versus self’ hybridizations). Furthermore, these effects are species-specific, with S. japonicum or Mus musculus gDNA failing to bind significantly to S. mansoni oligonucleotide DNA microarrays (e.g R = 0.350 when S. mansoni gDNA is co-hybridized with S. japonicum gDNA). Increased median fluorescent intensities (209.9) were also observed for DNA microarray elements hybridized with S. mansoni gDNA compared to complex mixed pools of S. mansoni cDNA (112.2). Exploiting these valuable characteristics, S. mansoni gDNA was used in two-channel DNA microarray hybridization experiments as a common reference for indirect identification of gender-associated transcripts in cercariae, a schistosome life-stage in which there is no overt sexual dimorphism. This led to the identification of 2,648 gender-associated transcripts. When compared to the 780 gender-associated transcripts identified by hybridization experiments utilizing a two-channel direct method (co-hybridization of male and female cercariae cDNA), indirect methods using gDNA were far superior in identifying greater quantities of differentially expressed transcripts. Interestingly, both methods identified a concordant subset of 188 male-associated and 156 female-associated cercarial transcripts, respectively. Gene ontology classification of these differentially expressed transcripts revealed a greater diversity of categories in male cercariae. Quantitative real-time PCR analysis confirmed the DNA microarray results and supported the reliability of this platform for identifying gender-associated transcripts. CONCLUSIONS/SIGNIFICANCE: Schistosome gDNA displays characteristics highly suitable for the comparison of two-channel DNA microarray results obtained from experiments conducted independently across laboratories. The schistosome transcripts identified here demonstrate, for the first time, that gender-associated patterns of expression are already well established in the morphologically identical, but chromosomally distinct, cercariae stage
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