Colicin Z, a structurally and functionally novel colicin type that selectively kills enteroinvasive Escherichia coli and Shigella strains

Colicin production in Escherichia coli (E. coli) strains represents an important trait with regard to microbial survival and competition in the complex intestinal environment. A novel colicin type, colicin Z (26.3 kDa), was described as a product of an original producer, extraintestinal E. coli B1356 strain, isolated from the anorectal abscess of a 17 years-old man. The 4,007 bp plasmid (pColZ) was completely sequenced and colicin Z activity (cza) and colicin Z immunity (czi) genes were identified. The cza and czi genes are transcribed in opposite directions and encode for 237 and 151 amino acid-long proteins, respectively. Colicin Z shows a narrow inhibitory spectrum, being active only against enteroinvasive E. coli (EIEC) and Shigella strains via CjrC receptor recognition and CjrB- and ExbB-, ExbD-mediated colicin translocation. All tested EIEC and Shigella strains isolated between the years 1958–2010 were sensitive to colicin Z. The lethal effect of colicin Z was found to be directed against cell wall peptidoglycan (PG) resulting in PG degradation, as revealed by experiments with Remazol Brilliant Blue-stained purified peptidoglycans and with MALDI-TOF MS analyses of treated PG. Colicin Z represents a new class of colicins that is structurally and functionally distinct from previously studied colicin types

Sulfuric acid speleogenesis and surface landform evolution along the Vienna Basin Transfer Fault: Plavecký Karst, Slovakia

Hypogene caves in the Plavecký hradný vrch Hill (Western Slovakia, Central Europe) were formed by waters ascending along faults in fractured Triassic carbonates related to the horst-graben structure at the contact of the Malé Karpaty Mountains and the NE part of the Vienna Basin. The Plavecká jaskyňa and Pec caves mostly contain horizontal passages and chambers with flat corrosion bedrock floors, fissure discharge feeders, wall water-table notches, replacement pockets, as well as a few other speleogens associated with sulfuric acid speleogenesis. The low-temperature sulfuric acid development phases of the Plavecká Jaskyňa are also indicated by the presence of sulfate minerals (i.e., gypsum and jarosite).Subaerial calcite popcorn rims were precipitated from water condensation at the edges of feeding fissures that were still active as thermal vents when the water table dropped. Hydrogen sulfide involved in the sulfuric acid speleogenesis was likely derived from anhydrites and/or hydrocarbon reservoirs with sulfate-saline connate waters in the fill of the adjacent Vienna Basin. It ascended to the surface along deep-rooted sub-vertical fault zones at the contact of the Vienna Basin with neighboring mountains. Three cave levels at 295 to 283 m asl in the Pec Cave, and five levels at 225 to 214 m asl in the Plavecká jaskyňa corresponded to phases of stable local erosional base levels in the bordering part of the Vienna Basin, most likely during periods of strongly decelerated and/or interrupted subsidence. Cave levels separated by vertical differences of only a few meters may also be related to the Pleistocene climatic cycles. The subhorizontal parts of the Pec Cave are probably of late Early Pleistocene age (˃0.99–1.07 Ma?). The two highest levels of the Plavecká jaskyňa developed during the early Middle Pleistocene (˃600 ka). Fine-grained sediments in the passage at 225 m asl with normal magnetic polarity contain jarosite. The middle level of the Plavecká jaskyňa at 220 m asl was formed in the mid-Middle Pleistocene, while the lower and lowermost levels formed in the late Middle Pleistocene (˃270 ka). The water table in the lowermost cave level probably dropped after the tectonic reactivation of the Podmalokarpatská zníženina Depression just in the front of a marginal horst structure of the Malé Karpaty Mountains

Sulfuric acid speleogenesis and surface landform evolution along the Vienna Basin Transfer Fault: Plavecký Karst, Slovakia

Hypogene caves in the Plavecký hradný vrch Hill (Western Slovakia, Central Europe) were formed by waters ascending along faults in fractured Triassic carbonates related to the horst-graben structure at the contact of the Malé Karpaty Mountains and the NE part of the Vienna Basin. The Plavecká jaskyňa and Pec caves mostly contain horizontal passages and chambers with flat corrosion bedrock floors, fissure discharge feeders, wall water-table notches, replacement pockets, as well as a few other speleogens associated with sulfuric acid speleogenesis. The low-temperature sulfuric acid development phases of the Plavecká Jaskyňa are also indicated by the presence of sulfate minerals (i.e., gypsum and jarosite).Subaerial calcite popcorn rims were precipitated from water condensation at the edges of feeding fissures that were still active as thermal vents when the water table dropped. Hydrogen sulfide involved in the sulfuric acid speleogenesis was likely derived from anhydrites and/or hydrocarbon reservoirs with sulfate-saline connate waters in the fill of the adjacent Vienna Basin. It ascended to the surface along deep-rooted sub-vertical fault zones at the contact of the Vienna Basin with neighboring mountains. Three cave levels at 295 to 283 m asl in the Pec Cave, and five levels at 225 to 214 m asl in the Plavecká jaskyňa corresponded to phases of stable local erosional base levels in the bordering part of the Vienna Basin, most likely during periods of strongly decelerated and/or interrupted subsidence. Cave levels separated by vertical differences of only a few meters may also be related to the Pleistocene climatic cycles. The subhorizontal parts of the Pec Cave are probably of late Early Pleistocene age (˃0.99–1.07 Ma?). The two highest levels of the Plavecká jaskyňa developed during the early Middle Pleistocene (˃600 ka). Fine-grained sediments in the passage at 225 m asl with normal magnetic polarity contain jarosite. The middle level of the Plavecká jaskyňa at 220 m asl was formed in the mid-Middle Pleistocene, while the lower and lowermost levels formed in the late Middle Pleistocene (˃270 ka). The water table in the lowermost cave level probably dropped after the tectonic reactivation of the Podmalokarpatská zníženina Depression just in the front of a marginal horst structure of the Malé Karpaty Mountains

Fibroblast growth factor receptor influences primary cilium length through an interaction with intestinal cell kinase.

Vertebrate primary cilium is a Hedgehog signaling center but the extent of its involvement in other signaling systems is less well understood. This report delineates a mechanism by which fibroblast growth factor (FGF) controls primary cilia. Employing proteomic approaches to characterize proteins associated with the FGF-receptor, FGFR3, we identified the serine/threonine kinase intestinal cell kinase (ICK) as an FGFR interactor. ICK is involved in ciliogenesis and participates in control of ciliary length. FGF signaling partially abolished ICK's kinase activity, through FGFR-mediated ICK phosphorylation at conserved residue Tyr15, which interfered with optimal ATP binding. Activation of the FGF signaling pathway affected both primary cilia length and function in a manner consistent with cilia effects caused by inhibition of ICK activity. Moreover, knockdown and knockout of ICK rescued the FGF-mediated effect on cilia. We provide conclusive evidence that FGF signaling controls cilia via interaction with ICK