Narrative Processes Coding System: A Dialectical Constructivist Approach to Assessing Client Change Processes in Emotion-Focused Therapy of Depression

Drawing on a Dialectical Constructivist model of therapeutic change, this paper addresses the fundamental contributions of client narrative disclosure, emotional differentiation and reflexive meaning-making processes in emotion-focused treatments of depression. An overview of the multi-methodological steps undertaken to empirically investigate the contributions of client storytelling, emotional differentiation, and meaning-making processes, using the Narrative Processes Coding System (NPCS; Angus et al., 1999) are provided, followed by a summary of key research findings that informed the development of a narrative-informed approach to emotion-focused therapy of depression (Angus & Greenberg, 2011). Finally, therapy practice implications for the adoption of a research-informed approach to working with narrative and emotion processes in emotion-focused therapy are described and future research directions discussed

Pointing all around you : selection performance of mouse and ray-cast pointing in full-coverage displays

Funding: SurfNet (NSERC, Canada), EPSRC (Small Equipment Grant).As display environments become larger and more diverse - now often encompassing multiple walls and room surfaces - it is becoming more common that users must find and manipulate digital artifacts not directly in front of them. There is little understanding, however, about what techniques and devices are best for carrying out basic operations above, behind, or to the side of the user. We conducted an empirical study comparing two main techniques that are suitable for full-coverage display environments: mouse-based pointing, and ray-cast `laser' pointing. Participants completed search and pointing tasks on the walls and ceiling, and we measured completion time, path lengths and perceived effort. Our study showed a strong interaction between performance and target location: when the target position was not known a priori the mouse was fastest for targets on the front wall, but ray-casting was faster for targets behind the user. Our findings provide new empirical evidence that can help designers choose pointing techniques for full-coverage spaces.Postprin

The effectiveness of three dimensional interaction

Most interaction with computers today takes place in a two dimensional environment. Even when using three dimensional graphics applications, input is often still restricted to two dimensions. Many believe that the use of three dimensional input devices will alleviate this restriction and allow for a much more natural human-machine dialog. This thesis seeks to establish how factors dealing with visual feedback and task structure affect the ability to perform interactive tasks in a three dimensional virtual environment. The factors investigated were stereoscopic vision, motion parallax, stimulus arrangement and stimulus complexity. Four tasks were studied. These tasks were: point location, docking, line tracing and curve tracing. All the tasks used a six degree of freedom input device to control a pointer in a three dimensional virtual environment. Four experiments corresponding to the four tasks were conducted to investigate these factors. Among other things the results showed the following. Stereoscopic vision provided a strong benefit to positioning-based tasks, but this benefit was weakened in the case of tracing tasks. Motion parallax via head-tracking often had no effect upon task performance and where an effect was found it was often detrimental. The position of stimuli influenced performance across all of the tasks. The orientation of stimuli influenced performance in the task in which it was varied.Science, Faculty ofComputer Science, Department ofGraduat

A Study of Interactive 3D Point Location in a Computer Simulated Virtual Environment

The present study investigated the ability to interactively locate points in a three dimensional computer environment using a six degree of freedom input device. Four different visual feedback modes were tested: fixed viewpoint monoscopic perspective, fixed viewpoint stereoscopic perspective, head-tracked monoscopic perspective and head-tracked stereoscopic perspective. Targets were located at plus and minus 10cm along the X, Y or Z axes, from a fixed starting location. Data about the time to complete the task and positioning accuracy (error) are gathered for each trial. In addition, subjective feedback regarding the apparatus, visual mode and task difficulty was solicited from subjects. The results indicate that stereoscopic performance is superior to monoscopic performance and that asymmetries exist both across and within axes. Head tracking had no appreciable effect upon performance. Subjective feedback regarding performance is usually consistent with objective measures, but some in..

Droplet-microfluidics-assisted sequencing of HIV proviruses and their integration sites in cells from people on antiretroviral therapy.

The human immunodeficiency virus (HIV) integrates its genome into that of infected cells and may enter an inactive state of reversible latency that cannot be targeted using antiretroviral therapy. Sequencing such a provirus and the adjacent host junctions in individual cells may elucidate the mechanisms of the persistence of infected cells, but this is difficult owing to the 150-million-fold higher amount of background human DNA. Here we show that full-length proviruses connected to their contiguous HIV-host DNA junctions can be assembled via a high-throughput microfluidic assay where droplet-based whole-genome amplification of HIV DNA in its native context is followed by a polymerase chain reaction (PCR) to tag droplets containing proviruses for sequencing. We assayed infected cells from people with HIV receiving suppressive antiretroviral therapy, resulting in the detection and sequencing of paired proviral genomes and integration sites, 90% of which were not recovered by commonly used nested-PCR methods. The sequencing of individual proviral genomes with their integration sites could improve the genetic analysis of persistent HIV-infected cell reservoirs

Multiple Origins of Virus Persistence during Natural Control of HIV Infection

Targeted HIV cure strategies require definition of the mechanisms that maintain the virus. Here, we tracked HIV replication and the persistence of infected CD4 T cells in individuals with natural virologic control by sequencing viruses, T cell receptor genes, HIV integration sites, and cellular transcriptomes. Our results revealed three mechanisms of HIV persistence operating within distinct anatomic and functional compartments. In lymph node, we detected viruses with genetic and transcriptional attributes of active replication in both T follicular helper (TFH) cells and non-TFH memory cells. In blood, we detected inducible proviruses of archival origin among highly differentiated, clonally expanded cells. Linking the lymph node and blood was a small population of circulating cells harboring inducible proviruses of recent origin. Thus, HIV replication in lymphoid tissue, clonal expansion of infected cells, and recirculation of recently infected cells act together to maintain the virus in HIV controllers despite effective antiviral immunity

HIV silencing and cell survival signatures in infected T cell reservoirs

Rare CD4 T cells that contain HIV under antiretroviral therapy represent an important barrier to HIV cure1-3, but the infeasibility of isolating and characterizing these cells in their natural state has led to uncertainty about whether they possess distinctive attributes that HIV cure-directed therapies might exploit. Here we address this challenge using a microfluidic technology that isolates the transcriptomes of HIV-infected cells based solely on the detection of HIV DNA. HIV-DNA+ memory CD4 T cells in the blood from people receiving antiretroviral therapy showed inhibition of six transcriptomic pathways, including death receptor signalling, necroptosis signalling and antiproliferative Gα12/13 signalling. Moreover, two groups of genes identified by network co-expression analysis were significantly associated with HIV-DNA+ cells. These genes (n = 145) accounted for just 0.81% of the measured transcriptome and included negative regulators of HIV transcription that were higher in HIV-DNA+ cells, positive regulators of HIV transcription that were lower in HIV-DNA+ cells, and other genes involved in RNA processing, negative regulation of mRNA translation, and regulation of cell state and fate. These findings reveal that HIV-infected memory CD4 T cells under antiretroviral therapy are a distinctive population with host gene expression patterns that favour HIV silencing, cell survival and cell proliferation, with important implications for the development of HIV cure strategies