Andrological, pathologic, morphometric, and ultrasonographic findings in rams experimentally infected with Brucella ovis

AbstractBrucella ovis is considered the most important infectious cause of reproductive disorders in sheep. The disease is characterized by epididymitis, subfertility and infertility in rams. B. ovis occasionally results in abortion in ewes, as well. The aim of this study was to evaluate kinetic changes in the reproductive organs of rams experimentally infected with B. ovis. Nine rams were experimentally inoculated intrapreputially with 2mL of a suspension containing 1.2×109CFU (colony-forming units)/mL of B. ovis (strain ATCC25840). In addition, 50μL of a suspension containing 1.2×1010CFU/mL of the same B. ovis strain was inoculated into each conjunctival sac, resulting in 3.6×109CFU total per ram. Six of nine infected rams had developed clinical changes in the tail of the epididymis at 30 days post-infection (dpi), but these changes regressed in 50% of these rams. Ultrasound demonstrated an increase in the area of the tail of the epididymis (P<0.001), reduction in the area of the testes (P<0.001), and an increased length and width of the seminal vesicles (P<0.001) during the course of infection. A sperm granuloma was diagnosed on the basis of ultrasonography findings. Microscopically, there was epididymitis, testicular degeneration, and seminal vesiculitis. Inflammatory cells were detected in the semen even before the development of epididymitis. Moreover, inflammatory cells were also found in the semen of asymptomatic rams, indicating that the presence of leukocytes in the ejaculate is a valuable method for screening potential carriers of infections in the genital tract

Lipidomic profile as a non-invasive tool to predict endometrial receptivity in freeze-all cycles

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Echinoderms from Todos os Santos Bay and Aratu Bay (Bahia, Brazil)

Neste trabalho são registrados os equinodermos (exceto Holothuroidea) obtidos durante a execução do projeto "Marine Environmental Evaluation of the Todos os Santos Bay-MAREMBA" em julho/agosto de 1996 e durante o projeto "Geoecologia das comunidades bentônicas infralitorais da Baía de Todos os Santos (BTS), BA, Brasil: diversidade biótica e sedimentológica", (1997). Um total de 33 espécies pertencentes a 15 famílias foram registradas e re-descritas. A distribuição de Ophiactis brasiliensis Manso, 1988 e Amphiodia trychna H.L. Clark, 1918 é estendida para a região nordeste do Brasil. A classe Ophiuroidea foi dominante nas amostragens em termos de abundância e freqüência de ocorrência, em particular as espécies Amphiodia atra Stimpson, 1852, Amphipholis subtilis (Ljungman, 1867), Ophiactislymani Ljungman, 1871 e Ophiostigma isocanthum (Say, 1825). O asteróide Oreaster reticulatus (Linnaeus, 1758) é considerado vulnerável à exploração humana e foi escasso durante as coletas. Outras espécies encontradas na BTS, tais como os asteroides Luidia clathrata (Say, 1825) e L. senegalensis (Lamarck, 1816) e o equinóide Eucidaristribuloides (Lamarck, 1816) figuram na lista nacional de espécies de invertebrados aquáticos ameaçados de extinção._______________________________________________________________________________ ABSTRACT: We present the results of the studies on biodiversity of Echinodermata (except Holothuroidea) from the Projects "Marine Environmental Evaluation of the Todos os Santos Bay - MAREMBA" (July/August 1996) and "Geoecology of the Benthic Infralitoral Communities of Todos os Santos Bay (BTS), Bahia, Brazil" (1997). A total of 33 species from 15 families were registered and re-described. The distribution of Ophiactis brasiliensis Manso, 1988 and Amphiodia trychna H.L. Clark, 1918 is extended to northwestern Brazil. The class Ophiuroidea was dominant in samples in terms of abundance and frequency of occurrence, in particular Amphiodia atra Stimpson, 1852, Amphipholis subtilis (Ljungman, 1867), Ophiactislymani Ljungman, 1871and Ophiostigma isocanthum (Say, 1825). The asteroid Oreaster reticulatus (Linnaeus, 1758) is considered vulnerable to human exploration and was scarce during the surveys. Other species found at BTS, such the asteroids Luidia clathrata (Say, 1825) and L. senegalensis (Lamarck, 1816) and the echinoid Eucidaristribuloides (Lamarck, 1816)are included in the national list of threatened invertebrate species