Validation of Malay version of the COVID-19 burnout scale

Objectives: From early 2020, Malaysia had to implement nationwide lockdowns, quarantines, and other social distancing practices to contain the spread of COVID-19 virus, leading to symptoms of psychosocial burnout among the people. The aim of the present research is to develop and validate a Malay language version of the COVID-19 Burnout Scale (M-COVID-19-BS). Methods: This three-phase study was conducted among Malaysian population. Phase 1 involved forward and backward translations of the Scale to Malay language by four professional bilingual translators. In Phase 2, the new M-COVID-19-BS instrument was piloted on 30 participants who suggested minor lexical modifications. Phase 3 consisted of online recruiting of Malaysian citizens to answer a composite questionnaire comprising the M-COVID-19-BS, Copenhagen Burnout Inventory (CBI), World Health Organization Quality of Life Scale Abbreviated Version (WHOQOL-BREF), and Fear of COVID-19 Scale (FCV-19S). Data were statistically analyzed. Results: The composite four-part questionnaire in Malay was answered by N = 225 Malaysian citizens. The M-COVID-19-BS instrument demonstrated a good internal consistency (Cronbach’s alpha = 0.926) and had a unidimensional factor structure. M-COVID-19-BS scores positively correlated with the three CBI subscales, showing evidence of convergent validity. Negative correlation was reported between M-COVID-19-BS and WHOQOL-BREF, achieving discriminant validity. M-COVID-19-BS also exhibited moderate positive correlations with the FCV-19S, thus supporting its concurrent validity. Conclusions: Results demonstrate that M-COVID-19-BS is a valid and reliable instrument to assess burnout symptoms related to COVID-19 among Malay-speaking populations either collectively or as a self-care tool to detect burnout symptoms without needing to further burden the already overwhelmed Malaysian healthcare system.Published versio

Validation of the Malay Version of the COVID-19 Anxiety Scale in Malaysia

Background: Malaysians are disillusioned with the increased number of COVID-19 infection cases and the prolonged lockdown period. As a result of COVID-19 mitigation measurements, Malaysians are experiencing emotional and psychological consequences such as anxiety. Thus, there is an urgent need to have an instrument that could serve as a tool to assess the psychological impact of COVID-19 among Malaysians rapidly. Methods: This study aimed to adapt and validate the Malay version of the COVID-19 Anxiety Scale (M-CAS) among Malaysian adults. The back-to-back translation was done to produce a M-CAS. Following face validation, M-CAS was self-administered to 225 participants from Malaysia via an online survey. The participants also completed the Generalised Anxiety Disorder 7-item Scale (GAD-7), World Health Organization Quality of Life Scale, Abbreviated Version (WHOQOL-BREF) and the Fear of COVID-19 Scale (FCV-19S). Data analysis was conducted using Statistical Package for the Social Sciences and Analysis of a Moment Structures. The psychometric properties of the M-CAS were examined via Cronbach alpha and confrmatory factor analysis. M-CAS scores were compared with the other tools to provide external validity. Results: The statistical analysis revealed that the M-CAS demonstrated adequate internal consistency (Cronbach’s alpha = 0.890) and presented with a unidimensional factor structure. M-CAS scores were strongly correlated with GAD-7 (r = 0.511, P < 0.001) and FCV-19S (r = 0.652, P < 0.001). Lack of correlation between M-CAS and WHOQOL-BREF showed that M-CAS scores did not reflect perceived quality of life. Conclusion: The M-CAS is a valid and reliable tool in the Malay language that can be selfadministered among Malaysians to assess anxiety levels induced by COVID-19. The M-CAS has only 7 items and utilised little time in real-life clinical practice

Lung Cancer Induces NK Cell Contractility and Cytotoxicity Through Transcription Factor Nuclear Localization

10.3389/fcell.2022.871326Frontiers in Cell and Developmental Biology10Complete

Control experiments and results.

<p>All the possible combinations of different levels of functionalized AFM tips against different samples were tested to exclude non-specific interaction induced artifacts.</p><p>*BSA: Bovine serum albumin</p>†<p>PHA-E: Phytohemagglutinin E</p>‡<p>PEG: Polyethylene glycol</p

Schematic illustration of AFM tip functionalization chemistry and experimental configurations.

<p>Schematic illustration of AFM tip functionalization chemistry and experimental configurations.</p

Three dimensional confocal images of functionalized AFM tips stained with antibody conjugated with fluorescent dyes.

<p>(A) Freshly prepared AFM tip with TSP conjugated. (B) Unmodified AFM tip as a control. (C) Used but still functional tip. (D) Used and ‘dead’ tip. Images were taken using Nikon N1 confocal microscope equipped with a 100X oil immersion objective. Scale bars represent 5 µm.</p

Schematic of energy landscape of CD36 and TSP reconstructed from dynamic force spectroscopy data.

<p>Δ<i>G</i> is the original energy barrier proportional to log(1/<i>k_off</i>) whereas Δ<i>G*</i> is the force reduced energy barrier. <i>x_β</i> is the reactive compliance or barrier width and <i>F</i> the applied force.</p

Force spectra and the extrapolated kinetic parameters.

<p>(A) CD36-IRBCs interaction and TSP-IRBCs interaction at physiological temperature (B) CD36-IRBCs interaction and CD36-CIDR interaction at physiological temperature. Mean rupture forces within each binned window of loading rates were used as pooled measures to fit to Bell-Evan's model for the characteristic dynamic force spectra reconstruction.</p