Excavations at the Nubian royal town of Kerma: 1975-91

Kerma is an ancient city on the Nile in Middle Nubia, long known and the subject of renewed recent exploration. Its position, at the southern limit of Egyptian control, sets it strategically on the routes to the African interior. Its environment in the arid desert results in remarkable preservation of organic remain

Une méthode d'identification en dynamique des structures basée sur le filtre de Kalman et l'erreur en relation de comportement

National audienceCe travail est issu de la problématique industrielle d'EDF autour du comportement dynamique des structures en présence de méconnaissances de modèle. On propose une méthode basée sur l'utilisation conjointe du filtre de Kalman Unscented et de l'erreur en relation de comportement pour localiser et identifier les paramètres structuraux permettant de mieux représenter des mesures physiques. Un exemple numérique d'une poutre en béton contenant à la fois de l'endommagement structurel et des défauts de modélisation des conditions aux limites est présenté.See http://hal.archives-ouvertes.fr/docs/00/59/28/56/ANNEX/r_2DS4H59F.pd

Post-ovulatory ageing and egg quality: A proteomic analysis of rainbow trout coelomic fluid

BACKGROUND: In fish, oocyte post-ovulatory ageing is associated with egg quality decrease. During this period, eggs are held in the body cavity where they bath in a semi-viscous liquid known as coelomic fluid (CF). CF components are suspected to play a role in maintaining oocyte fertility and developmental competence (egg quality). However, CF proteic composition remains poorly studied. Thus rainbow trout CF proteome was studied during the egg quality decrease associated with oocyte post-ovulatory ageing. METHODS: High resolution two-dimensional gel electrophoresis was used to analyze the proteome of rainbow trout (Oncorhynchus mykiss) CF in relationship with the egg quality decrease associated with oocyte post-ovulatory ageing. A first experiment was performed using CF pools originating from 17 females sampled at ovulation as well as 7, 14 and 21 days later. These observations were verified using a second set of CF pools originating from 22 females sampled 5 and 16 days following ovulation. RESULTS: Approximately 200 protein spots of 10–105 kDa molecular mass and 3–10 pI were detected in CF samples. Several protein spots, while undetected at the time of ovulation, exhibited a progressive and strong accumulation in CF during post-ovulatory ageing. After silver-staining and Matrix-Assisted Laser Desorption Time Of Flight (MALDI-TOF) mass spectrometer analysis, some of these protein spots were identified as lipovitellin II fragments. CONCLUSIONS: These observations suggest that egg protein fragments accumulate in the CF during the post-ovulatory period and could therefore be used to detect egg quality defects associated with oocyte post-ovulatory ageing

Contemplation de la nature

Encabezamiento completado con Brunet, I, col. 1103Sign.: *-5*>8585<Antep.Port. con grab. calc