4 research outputs found
Alterations in innate immunity and epithelial cell differentiation are the molecular pillars of hidradenitis suppurativa
Background
The large unmet need of hidradenitis suppurativa/acne inversa (HS) therapy requires the elucidation of diseaseâdriving mechanisms and tissue targeting.
Objective
Robust characterization of the underlying HS mechanisms and detection of the involved skin compartments.
Methods
Hidradenitis suppurativa/acne inversa molecular taxonomy and key signalling pathways were studied by whole transcriptome profiling. Dysregulated genes were detected by comparing lesional and nonâlesional skin obtained from female HS patients and matched healthy controls using the Agilent array platform. The differential gene expression was confirmed by quantitative realâtime PCR and targeted protein characterization via immunohistochemistry in another set of female patients. HSâinvolved skin compartments were also recognized by immunohistochemistry.
Results
Alterations to key regulatory pathways involving glucocorticoid receptor, atherosclerosis, HIF1α and IL17A signalling as well as inhibition of matrix metalloproteases were detected. From a functional standpoint, cellular assembly, maintenance and movement, haematological system development and function, immune cell trafficking and antimicrobial response were key processes probably being affected in HS. Sixteen genes were found to characterize HS from a molecular standpoint (DEFB4, MMP1, GJB2, PI3, KRT16, MMP9, SERPINB4, SERPINB3, SPRR3, S100A8, S100A9, S100A12, S100A7A (15), KRT6A, TCN1, TMPRSS11D). Among the proteins strongly expressed in HS, calgranulinâA, calgranulinâB and serpinâB4 were detected in the hair root sheath, koebnerisin and connexinâ32 in stratum granulosum, transcobalaminâ1 in stratum spinosum/hair root sheath, small prolinârich proteinâ3 in apocrine sweat gland ducts/sebaceous glandsâducts and matrix metallopeptidaseâ9 in resident monocytes.
Conclusion
Our findings highlight a panel of immuneârelated drivers in HS, which influence innate immunity and cell differentiation in follicular and epidermal keratinocytes as well as skin glands.ISSN:0926-9959ISSN:1468-308
Which Health-Related Quality of Life Items Most Affect Acne Patients?
BACKGROUND: Health-related quality of life (HRQoL) assessment in patients with acne is recommended by several national guidelines. There are several acne-specific HRQoL instruments. OBJECTIVES: Participants of the European Academy of Dermatology and Venereology (EADV) Task Forces (TFs) on QoL and Patient Oriented Outcomes (PO) and Acne, Rosacea, and Hidradenitis Suppurativa (ARHS) agreed to scrutinize aspects of existing acne-specific HRQoL instruments for their relevance in international study. METHODS: Consensus agreement on items related to QoL was reached after an independent assessment by seven experts from the EADV TFs on QoL and PO, and a list of 97 items was prepared and proposed to a group of acne patients. In order to have data from patients to check if any important topics were overseen, another group of acne patients from participating countries was asked to list how acne influenced different aspects of their lives. RESULTS: Based on results obtained from 601 acne patients from nine countries, most of the items and topics showed low relevance for acne patients especially during the previous month or shorter time periods. Based on percentage of relevance and factor analysis, short (6 items) and long (45 items) lists of the most relevant topics were formed. CONCLUSION: Most of the items and topics from the initial list showed low relevance for acne patients. None of the identified acne-specific HRQoL instruments contain all the items that were deemed most relevant to acne patients. For this reason, participating members of the EADV TFs on QoL and PO, and ARHs are in the process of developing a new acne-specific HRQoL instrument