108 research outputs found
The production of H2S by pure culture wine yeasts
Production of H2S by 12 pure culture wine yeasts on different substrates including grape juice has been studied. Under these conditions low- and high-sulphite forming yeasts showed different patterns of H2S formation : When grown with sulphate or sulphite as the only sulphur source the strains could be divided not only into low- or high-sulphite (S02) formers but also into low- or high-sulphide (H2S) forming yeasts. Growth on the ABY solid medium also separated high- from lowsulphide formers. A deficiency of pantothenate produced a similar pattern though with increased levels of H2S. The addition of L-cysteine with or without pantothenate caused a substantial increase in H 2S production with only some strains. Addition of colloidal sulphur produced high concentrations of H2S with all strains, such that they could not be differentiated. Neither did the fermentation of grape juice nor the addition of grape juice slurry to the synthetic substrate allow any differentiation of the yeasts. lt is concluded that H2S formation in grape juice comes mostly from residual colloidal sulphur from fungal sprays. It is also shown that more suitable yeast strains can be selected.Die Bildung von H2S durch ReinzuchthefenDie Bildung von H2S durch 12 Reinzuchthefen wurde mit verschiedenen GĂ€rsubstratzusammensetzungen untersucht. Wenig- und viel-Sulfitbildende Hefen zeigten unterschiedliches H2S-Bildungsvermögen: Waren Sulfat oder Sulfit die einzigen Schwefelquellen, dann konnten die Hefen nicht nur in Wenig- und Viel-Sulfit-Bildner, sondern auch in wenig- und viel-Sulfid-bildende StĂ€mme eingeteilt werden. Letzteres zeigte sich auch, wenn die Hefen auf ABY-Agar ausgestrichen wurden. Auch ein Pantothenat-Mangelsubstrat bewirkte im wesentlichen die gleichen Ergebnisse. Wurde Cystein zugegeben, mit oder ohne Pantothenat, so reagierten nur einige StĂ€mme mit verstĂ€rkter H2S-Bildung. Alle HefestĂ€mme produzierten gleich viel H2S, wenn dem GĂ€rsubstrat kolloidaler Schwefel zugegeben worden war. Die VergĂ€rung von Traubenmost oder von synthetischem Substrat, dem Zentrifugentrub zugesetzt worden war, ermöglichte ebenfalls keine Gruppierung der StĂ€mme. Es wird vermutet, daĂ die H2S-Bildung bei der VergĂ€rung von Traubensaft meistens durch kolloidalen Schwefel aus SpritzmittelrĂŒckstĂ€nden verursacht wird. Es wird auĂerdem gezeigt, daĂ geeignetere Reinzuchthefen selektioniert werden können
Effect of Rhizobial Strain and Host Plant on Nitrogen Isotopic Fractionation in Legumes
Glucagon-like peptide-1 (GLP-1) and the regulation of human invariant natural killer T cells: lessons from obesity, diabetes and psoriasis
Aims/hypothesis The innate immune cells, invariant natural
killer T cells (iNKT cells), are implicated in the pathogenesis
of psoriasis, an inflammatory condition associated with
obesity and other metabolic diseases, such as diabetes and
dyslipidaemia. We observed an improvement in psoriasis severity in a patient within days of starting treatment with an
incretin-mimetic, glucagon-like peptide-1 (GLP-1) receptor
agonist. This was independent of change in glycaemic control.
We proposed that this unexpected clinical outcome resulted
from a direct effect of GLP-1 on iNKTcells.
Methods We measured circulating and psoriatic plaque
iNKT cell numbers in two patients with type 2 diabetes
and psoriasis before and after commencing GLP-1 analogue
therapy. In addition, we investigated the in vitro effects of
GLP-1 on iNKT cells and looked for a functional GLP-1
receptor on these cells.
Results The Psoriasis Area and Severity Index improved in
both patients following 6 weeks of GLP-1 analogue
therapy. This was associated with an alteration in iNKT
cell number, with an increased number in the circulation
and a decreased number in psoriatic plaques. The GLP-1
receptor was expressed on iNKT cells, and GLP-1 induced
a dose-dependent inhibition of iNKT cell cytokine secretion,
but not cytolytic degranulation in vitro.
Conclusions/interpretation The clinical effect observed and
the direct interaction between GLP-1 and the immune
system raise the possibility of therapeutic applications for
GLP-1 in inflammatory conditions such as psoriasis
Expression of the chemokine receptor CCR5 in psoriasis and results of a randomized placebo controlled trial with a CCR5 inhibitor
Several reports have indicated that the chemokine receptor CCR5 and its ligands, especially CCL5 (formerly known as RANTES), may play a role in the pathogenesis of psoriasis. The purpose of this investigation was to examine the expression of CCR5 and its ligands in chronic plaque psoriasis and to evaluate the clinical and immunohistochemical effect of a CCR5 receptor inhibitor. Immunohistochemical analysis showed low but significant increased total numbers of CCR5 positive cells in epidermis and dermis of lesional skin in comparison to non-lesional skin. However, relative expression of CCR5 proportional to the cells observed revealed that the difference between lesional and non-lesional skin was only statistically significant in the epidermis for CD3 positive cells and in the dermis for CD68 positive cells. Quantification of mRNA by reverse transcriptase-polymerase chain reaction only showed an increased expression of CCL5 (RANTES) in lesional skin. A randomized placebo-controlled clinical trial in 32 psoriasis patients revealed no significant clinical effect and no changes at the immunohistochemical level comparing patients treated with placebo or a CCR5 inhibitor SCH351125. We conclude that although CCR5 expression is increased in psoriatic lesions, this receptor does not play a crucial role in the pathogenesis of psoriasis
Expression Patterns of Coagulation Factor XIII Subunit A on Leukemic Lymphoblasts Correlate with Clinical Outcome and Genetic Subtypes in Childhood B-cell Progenitor Acute Lymphoblastic Leukemia
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Nitrogen losses from soils caused by Rhizobium-dependent denitrification
Biological nitrogen fixation by the Rhizobium-legume symbiosis is of considerable agronomic importance, and most research on rhizobia is aimed at a better understanding of symbiotic nitrogen fixation (e.g. Hardy er nl.. 1979; Hardy and Silver. 1977: Hardv and Gibson. 1977). However the d I significance of early observations on denitrification by rhizobia (Rajagopalan, 1938; Ishizawa, 1939; Wilson, 1947) appears to have been overlooked; it has recently been shown to be widespread among Rhizobium species (Zablotowicz et al., 1978; Daniel ef al., 1980a. 1982) and to occur in soils under laboratory conditions (Daniel el al., 1980b). We report here field experiments showing the loss of fixed nitrogen from soils by denitrification carried out by rhizobia at rates comparable to those at which rhizobia fix nitrogen. This occurs in aerated soils, unlike previously described soil denitrification systems which are considered to be anaerobic (Knowles, 1982). Rhizobia are widespread in agricultural ecosystems (Parker et al., 1977) and an understanding of the conditions under which they denitrify may convey substantial benefits
Effect of Rhizobial Strain and Host Plant on Nitrogen Isotopic Fractionation in Legumes
Lotus pedunculatus L., Medicago sativa L., Macroptilium atropurpureum, Glycine max, and Trifolium repens L. were grown in a N-free medium and inoculated with one of ten Rhizobium strains. Dry matter, N content, and ÎŽ(15)N values were determined for various plant parts. Nodules, with the exception of those from lucerne, were enriched in (15)N relative to atmospheric N. Considerable variation was found in ÎŽ(15)N values of plant herbage (â4.5 to +0.8). The extent of isotopic discrimination was dependent on both the host plant and the infecting rhizobial strain. This further complicates, but does not invalidate, the use of small variations in the natural abundance of (15)N to estimate the contribution of symbiotically fixed N(2) to the N in legume herbage. Some other implications of the observed differences are also discussed
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