35 research outputs found
Selective Down-Regulation of Nuclear Poly(ADP-Ribose) Glycohydrolase
The formation of ADP-ribose polymers on target proteins by poly(ADP-ribose) polymerases serves a variety of cell signaling functions. In addition, extensive activation of poly(ADP-ribose) polymerase-1 (PARP-1) is a dominant cause of cell death in ischemia-reperfusion, trauma, and other conditions. Poly(ADP-ribose) glycohydrolase (PARG) degrades the ADP-ribose polymers formed on acceptor proteins by PARP-1 and other PARP family members. PARG exists as multiple isoforms with differing subcellular localizations, but the functional significance of these isoforms is uncertain.Primary mouse astrocytes were treated with an antisense phosphorodiamidate morpholino oligonucleotide (PMO) targeted to exon 1 of full-length PARG to suppress expression of this nuclear-specific PARG isoform. The antisense-treated cells showed down-regulation of both nuclear PARG immunoreactivity and nuclear PARG enzymatic activity, without significant alteration in cytoplasmic PARG activity. When treated with the genotoxic agent MNNG to induced PARP-1 activation, the antisense-treated cells showed a delayed rate of nuclear PAR degradation, reduced nuclear condensation, and reduced cell death.These results support a preferentially nuclear localization for full-length PARG, and suggest a key role for this isoform in the PARP-1 cell death pathway
The role of the intensive care unit in real-time surveillance of emerging pandemics: The Italian GiViTI experience
Gro fstadt Architektur. Milano Scalo Farini.
Il progetto nasce dal lavoro condotto in occasione del Workshop "Milano. Scali ferroviari e trasformazioni urbane" svoltosi nel settembre 2009 presso la Facolt\ue0 di Architettura Civile del Politecnico di Milano, in collaborazione con l'Assessorato allo sviluppo del Territorio del Comune di Milano e AD Sistemi Urbani S.r.l. del Gruppo Ferrovie dello Stato. La proposta si sviluppa a partire dei vincoli imposti dall'Accordo di Programma tra Comune, Regione e Fs Spa del 10 marzo 2009
Su storia e discorso in hegel
Il saggio si propone di mettere in luce, a partire da alcuni lavori di G. Lebrun e S. Zizek, il rapporto tra sistema e dialettica nella filosofia di Hegel, rapporto che si ritiene non contraddittorio