Fast HPLC analysis of omeprazole, 5-hydroxyomeprazole and omeprazole sulfone in liquid culture medium using a monolithic column for application in biotransformation studies with fungi

A fast liquid chromatography method was developed and validated for the simultaneous determination of omeprazole (OMZ), 5-hydroxyomeprazole (5-HOMZ) and omeprazole sulphone (OMZ SUL) in liquid culture medium for application in biotransformation studies employing phytopathogenic and endophytic fungi. The separation was achieved using a monolithic Chromolith Fast gradient RP 18 endcapped column, using a mobile phase consisting of 0.15% (v/v) trifluoroacetid acid (TFA) in water (solvent A) and 0.15% (v/v) TFA in acetonitrile (solvent B), under linear gradient of 5 to 90% of B in 1 min, flow rate of 1.0 mL min-1, temperature at 30 ºC and detection at 220 nm. Sample preparation was performed by liquid-liquid extraction, with recoveries in the range of 62.3 to 76.6% for all analytes. The method was linear in the range of 0.2 to 10.0 µg mL-1 (r &#8805; 0.995). The values for intra- and inter-day precision (% coefficient of variation) and accuracy (% relative error) were < 15% for all analytes. The validated method was used to evaluate OMZ biotransformation to their mammalian metabolites by selected fungi. In general, the phytopathogenic fungi studied were more efficient to biotransform OMZ. The sulfonation reaction was more prevalent for all studied fungi.Um método rápido por cromatografia líquida foi desenvolvido para a determinação simultânea de omeprazol (OMZ), 5-hidroxiomeprazol (5-HOMZ) e omeprazol sulfona (OMZ SUL) em meio de cultura líquido, para aplicação em estudos de biotransformação empregando fungos fitopatogênicos e endofíticos. A separação foi realizada empregando uma coluna monolítica Chromolith Fast gradient RP 18 com a fase móvel constituída por ácido trifluoroacético (TFA) 0,15% (v/v) em água (solvente A) e TFA 0,15% (v/v) em acetonitrila (solvente B). Foi empregado um gradiente linear de 5 a 90% de B em 1 minuto, vazão de 1,0 mL min-1, temperatura de 30 ºC e detecção em 220 nm. A extração líquido-líquido foi empregada na preparação das amostras, com recuperações na faixa de 62,3-76,6% para todos os analitos. O método foi linear na faixa de 0,2-10,0 µg mL-1 (r &#8805; 0,995). Os valores de precisão e exatidão intra- e inter-dias (coeficiente de variação e erro relativo) foram inferiores a 15% para todos os analitos. O método validado foi utilizado para avaliar a biotransformação do OMZ em seus principais metabólitos humanos pelos fungos selecionados. Em geral, os fungos fitopatogênicos foram mais eficientes para biotransformar o OMZ. A reação de sulfonação foi mais prevalente em todos os fungos estudadosFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Quantum properties of dichroic silicon vacancies in silicon carbide

The controlled generation and manipulation of atom-like defects in solids has a wide range of applications in quantum technology. Although various defect centres have displayed promise as either quantum sensors, single photon emitters or light-matter interfaces, the search for an ideal defect with multi-functional ability remains open. In this spirit, we investigate here the optical and spin properties of the V1 defect centre, one of the silicon vacancy defects in the 4H polytype of silicon carbide (SiC). The V1 centre in 4H-SiC features two well-distinguishable sharp optical transitions and a unique S=3/2 electronic spin, which holds promise to implement a robust spin-photon interface. Here, we investigate the V1 defect at low temperatures using optical excitation and magnetic resonance techniques. The measurements, which are performed on ensemble, as well as on single centres, prove that this centre combines coherent optical emission, with up to 40% of the radiation emitted into the zero-phonon line (ZPL), a strong optical spin signal and long spin coherence time. These results single out the V1 defect in SiC as a promising system for spin-based quantum technologies

Adiabatic quantum algorithm for search engine ranking

We propose an adiabatic quantum algorithm for generating a quantum pure state encoding of the PageRank vector, the most widely used tool in ranking the relative importance of internet pages. We present extensive numerical simulations which provide evidence that this algorithm can prepare the quantum PageRank state in a time which, on average, scales polylogarithmically in the number of webpages. We argue that the main topological feature of the underlying web graph allowing for such a scaling is the out-degree distribution. The top ranked $\log(n)$ entries of the quantum PageRank state can then be estimated with a polynomial quantum speedup. Moreover, the quantum PageRank state can be used in "q-sampling" protocols for testing properties of distributions, which require exponentially fewer measurements than all classical schemes designed for the same task. This can be used to decide whether to run a classical update of the PageRank.Comment: 7 pages, 5 figures; closer to published versio

Extração de DNA genômico de Brachiaria e Panicum maximum.

bitstream/CNPGC/10572/1/COT79.pd

Administration of hCG on the seventh day after initiation of estrus may circumvent negative effects of cervical relaxation protocol in sheep: Preliminary results.

Cervical relaxation with association of estradiol benzoate, cloprostenol and oxytocin allows a cervical transposition and embryo collection in sheep by the non-surgical method (Fonseca et al., Theriogenology, 86: 144-151, 2016). However, in recipients, the use of this protocol becomes impracticable due to the use of cloprostenol, which is a luteolytic agent. Sheep are known to form accessory luteal bodies after hCG administration seven days after the onset of estrus (Castro et al., Anim. Reprod., 12: 148, 2015). Therefore, the objective of this study was to develop a cervical relaxation protocol in embryo recipient ewes without compromising future gestation. The data were presented in a descriptive way. Thirteen ewes were observed in estrus out of 24 females submitted to the synchronization protocol. The pregnancy rate were 75.0% (3/4) in G1, 0.0% in G2 (0/5) and 50.0% in G3 (2/4). Preliminary results suggest that the association of estradiol benzoate and oxytocin may compromise the onset of pregnancy and that the use of hCG at the end of the protocol in D7 can circumvent partially these negative effects, allowing the establishment of pregnancy

Intravaginal hCG administration increases pregnancy rate in artificially inseminated cyclic dairy goats subjected to estrous synchronization.

Pregnancy establishing after artificial insemination (AI) in goats depends on many important aspects, including time of AI, ovulation and premature luteal regression. The knowledge of ovulation in relation to estrus onset, ovulation induction and strategies to prevent PLR can be valuable tools to increase pregnancy rate. This study tested the effect of hCG administration on pregnancy rate in artificially inseminated dairy cyclic goats after estrus synchronization Results of this study showed that hCG administration associated to AI significantly increased pregnancy rates in goats after estrus synchronization with dcloprostenol, which can be a valuable and promising tool to be applied in field conditions

Estimativa do volume de madeira em unidades de referência tecnológica com sistema agrossilvipastoril.

Este trabalho tem como objetivo apresentar resultados de inventário florestal, com enfoque no volume de madeira e no incremento médio anual (IMA) de madeira em sistemas Agrossilvipastoris, em quatro áreas (URTs) localizadas nos municípios de Porto Vitória-PR, Caseiros-RS e Passo Fundo-RS. O inventário florestal foi realizado em novembro de 2012, mensurando a altura das plantas com utilização de hipsômetro e avaliando o diâmetro do tronco na altura do peito (DAP), utilizando sutas de precisão. Os dados foram processados para determinação da altura média das árvores, diâmetro médio na altura do peito, volume médio por árvore, volume por hectare (ha), volume total da área com sistema silvipastoril e incremento médio anual em volume. Também, foi estimado o volume de madeira a ser colhido com base na simulação de quatro diferentes intensidades de desbaste: 25%, 30%, 35% e 40%. No sistema silvipastoril o volume de madeira é variável em função da localização, densidade de plantio e manejo do sistema. O incremento médio anual de madeira, estimada com base no volume total de madeira das URTs, variou de 13,6 a 16,6 m3.ha-1.ano em plantios com quatro anos de idade em Caseiros-RS e Passo Fundo-RS e de 19,7 a 22,9 m3.ha-1.ano em plantios com 6 anos de idade em Porto Vitória-PR, considerando os respectivos espaçamentos. É possível obter um volume total de madeira entre 118 a 137m3 ha-1 em plantios com seis anos e 54 a 66,8 m3 ha-1 em plantios com quatro anos, variável conforme a densidade de plantio, as condições de manejo e percentuais de sobrevivência. O produtor pode obter uma renda extra oriunda do desbaste, que irá variar de acordo com as condições de mercado e com a intensidade do desbaste