161 research outputs found
The brachyopoid Hadrokkosaurus bradyi from the early Middle Triassic of Arizona, and a phylogenetic analysis of lower jaw characters in temnospondyl amphibians
The holotype of the brachyopoid temnospondyl Hadrokkosaurus bradyi, represented by a right lower jaw ramus, is reâexâ
amined based upon new data and revision of various morphological features. Additional fragmentary jaw material reâ
ferred to this species is briefly described. Prominent features are a large postsymphyseal foramen that is anteriorly open,
and prearticular and surangular buttresses for support of the articular. Brachyopoid characters include a long and robust
postglenoid area formed by surangular and prearticular, anterior and posterior keels on at least some marginal dentary
teeth, and subtriangular outline of the adductor fossa in dorsal view. Five features of the holotype ramus, long thought to
be at odds with its brachyopoid or temnospondyl nature, are critically reâevaluated. A phylogenetic analysis of lower jaw
characters in temnospondyls retrieves most of the clades found in more comprehensive data sets, but the statistical node
support is low. Brachyopoids are monophyletic, with Hadrokkosaurus emerging as their most basal taxon
Superhumps in Cataclysmic Binaries. XXIII. V442 Ophiuchi and RX J1643.7+3402
We report the results of long observing campaigns on two novalike variables:
V442 Ophiuchi and RX J1643.7+3402. These stars have high-excitation spectra,
complex line profiles signifying mass loss at particular orbital phases, and
similar orbital periods (respectively 0.12433 and 0.12056 d). They are
well-credentialed members of the SW Sex class of cataclysmic variables. Their
light curves are also quite complex. V442 Oph shows periodic signals with
periods of 0.12090(8) and 4.37(15) days, and RX J1643.7+3402 shows similar
signals at 0.11696(8) d and 4.05(12) d. We interpret these short and long
periods respectively as a "negative superhump" and the wobble period of the
accretion disk. The superhump could then possibly arise from the heating of the
secondary (and structures fixed in the orbital frame) by inner-disk radiation,
which reaches the secondary relatively unimpeded since the disk is not
coplanar.
At higher frequencies, both stars show another type of variability:
quasi-periodic oscillations (QPOs) with a period near 1000 seconds. Underlying
these strong signals of low stability may be weak signals of higher stability.
Similar QPOs, and negative superhumps, are quite common features in SW Sex
stars. Both can in principle be explained by ascribing strong magnetism to the
white dwarf member of the binary; and we suggest that SW Sex stars are
borderline AM Herculis binaries, usually drowned by a high accretion rate. This
would provide an ancestor channel for AM Hers, whose origin is still
mysterious.Comment: PDF, 41 pages, 4 tables, 16 figures; accepted, in press, to appear
December 2002, PASP; more info at http://cba.phys.columbia.edu
The legal and ethical framework governing Body Donation in Europe-1st update on current practice
Previously, we have reported on the legal and
ethical aspects and current practice of body
donation in several European countries,
reflecting cultural and religious variations as
well as different legal and constitutional
frameworks. We have also established good
practice in body donation. Here we shall further extend the legal and ethical frameworks
in place and also focus on novelties in the law
and different directives. Of particular interest
are points that address the commercialization
of human bodies and body parts and weaknesses in the legal directives. Therefore, it is
important to define what is ethical and what
needs to be considered unethical in body
donation and the subsequent utilisation of
human bodies for teaching and research.peer-reviewe
mRNA expression profiles in circulating tumor cells of metastatic colorectal cancer patients
The molecular characterization of circulating tumor cells (CTCs) is a promising tool for the repeated and non-invasive evaluation of predictive and prognostic factors. Challenges associated with CTC characterization using the only FDA approved method for CTC enumeration, the CellSearch technique, include the presence of an excess of leukocytes in CTC-enriched blood fractions. Here we aimed to identify colorectal tumor-specific gene expression levels in the blood of patients with and without detectable CTCs according to CellSearch criteria. Materials and methods: Blood of 30 healthy donors (HDs) and 142 metastatic colorectal cancer (mCRC) patients was subjected to CellSearch CTC enumeration and isolation. In all samples, 95 mRNAs were measured by reverse transcriptase quantitative PCR (RT-qPCR). HD blood samples and patient samples with three or more CTCs were compared to identify CTC-specific mRNAs. Patient samples without detectable CTCs were separately analyzed. Results: Thirty-four CTC-specific mRNAs were higher expressed in patients with â„3 CTCs compared with HDs (Mann-Whitney U-test P<0.05). Among patients without detectable CTCs, a HD-unlike subgroup was identified which could be distinguished from HDs by the expression of epithelial genes such as KRT19, KRT20 and AGR2. Also, in an independent patient set, a similar HD-unlike group could be identified among the patients without detectable CTCs according to the CellSearch system. Conclusion: Extensive molecular characterization of colorectal CTCs is feasible and a subgroup of patients without detectable CTCs according to CellSearch criteria bears circulating tumor load, which may have clinical consequences. This CTC-specific gene panel for mCRC patients may enable the exploration of CTC characterization as a novel means to further individualize cancer treatment
Molecular characteristics of circulating tumor cells resemble the liver metastasis more closely than the primary tumor in metastatic colorectal cancer
Background: CTCs are a promising alternative for metastatic tissue biopsies for use in precision medicine approaches. We investigated to what extent the molecular characteristics of circula
Superhumps in Cataclysmic Binaries. XXIV. Twenty More Dwarf Novae
We report precise measures of the orbital and superhump period in twenty more
dwarf novae. For ten stars, we report new and confirmed spectroscopic periods -
signifying the orbital period P_o - as well as the superhump period P_sh. These
are GX Cas, HO Del, HS Vir, BC UMa, RZ Leo, KV Dra, KS UMa, TU Crt, QW Ser, and
RZ Sge. For the remaining ten, we report a medley of P_o and P_sh measurements
from photometry; most are new, with some confirmations of previous values.
These are KV And, LL And, WX Cet, MM Hya, AO Oct, V2051 Oph, NY Ser, KK Tel, HV
Vir, and RX J1155.4-5641.
Periods, as usual, can be measured to high accuracy, and these are of special
interest since they carry dynamical information about the binary. We still have
not quite learned how to read the music, but a few things are clear. The
fractional superhump excess epsilon [=(P_sh-P_o)/P_o] varies smoothly with P_o.
The scatter of the points about that smooth curve is quite low, and can be used
to limit the intrinsic scatter in M_1, the white dwarf mass, and the
mass-radius relation of the secondary. The dispersion in M_1 does not exceed
24%, and the secondary-star radii scatter by no more than 11% from a fixed
mass-radius relation. For the well-behaved part of epsilon(P_o) space, we
estimate from superhump theory that the secondaries are 18+-6% larger than
theoretical ZAMS stars. This affects some other testable predictions about the
secondaries: at a fixed P_o, it suggests that the secondaries are (compared
with ZAMS predictions) 40+-14% less massive, 12+-4% smaller, 19+-6% cooler, and
less luminous by a factor 2.5(7). The presence of a well-defined mass-radius
relation, reflected in a well-defined epsilon(P_o) relation, strongly limits
effects of nuclear evolution in the secondaries.Comment: PDF, 62 pages, 7 tables, 21 figures; accepted, in press, to appear
November 2003, PASP; more info at http://cba.phys.columbia.edu
A cell atlas of human thymic development defines T cell repertoire formation.
The thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We used single-cell RNA sequencing to create a cell census of the human thymus across the life span and to reconstruct T cell differentiation trajectories and T cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ CD8αα+ T cell populations, thymic fibroblast subtypes, and activated dendritic cell states. In addition, we reveal a bias in TCR recombination and selection, which is attributed to genomic position and the kinetics of lineage commitment. Taken together, our data provide a comprehensive atlas of the human thymus across the life span with new insights into human T cell development
Cells of the human intestinal tract mapped across space and time
Acknowledgements We acknowledge support from the Wellcome Sanger Cytometry Core Facility, Cellular Genetics Informatics team, Cellular Generation and Phenotyping (CGaP) and Core DNA Pipelines. This work was financially supported by the Wellcome Trust (W1T20694, S.A.T.; 203151/Z/16/Z, R. A. Barker.); the European Research Council (646794, ThDefine, S.A.T.); an MRC New Investigator Research Grant (MR/T001917/1, M.Z.); and a project grant from the Great Ormond Street Hospital Childrenâs Charity, Sparks (V4519, M.Z.). The human embryonic and fetal material was provided by the Joint MRC/Wellcome (MR/R006237/1) Human Developmental Biology Resource (https://www.hdbr.org/). K.R.J. holds a Non-Stipendiary Junior Research Fellowship from Christâs College, University of Cambridge. M.R.C. is supported by a Medical Research Council Human Cell Atlas Research Grant (MR/S035842/1) and a Wellcome Trust Investigator Award (220268/Z/20/Z). H.W.K. is funded by a Sir Henry Wellcome Fellowship (213555/Z/18/Z). A.F. is funded by a Wellcome PhD Studentship (102163/B/13/Z). K.T.M. is funded by an award from the Chan Zuckerberg Initiative. H.H.U. is supported by the Oxford Biomedical Research Centre (BRC) and the The Leona M. and Harry B. Helmsley Charitable Trust. We thank A. Chakravarti and S. Chatterjee for their contribution to the analysis of the enteric nervous system. We also thank R. Lindeboom and C. Talavera-Lopez for support with epithelium and Visium analysis, respectively; C. Tudor, T. Li and O. Tarkowska for image processing and infrastructure support; A. Wilbrey-Clark and T. Porter for support with Visium library preparation; A. Ross and J. Park for access to and handling of fetal tissue; A. Hunter for assistance in protocol development; D. Fitzpatrick for discussion on developmental intestinal disorders; and J. Eliasova for the graphical images. We thank the tissue donors and their families, and the Cambridge Biorepository for Translational Medicine and Human Developmental Biology Resource, for access to human tissue. This publication is part of the Human Cell Atlas: https://www.humancellatlas.org/publications.Peer reviewedPublisher PD
Cells of the human intestinal tract mapped across space and time.
Funder: Medical Research CouncilThe cellular landscape of the human intestinal tract is dynamic throughout life, developing in utero and changing in response to functional requirements and environmental exposures. Here, to comprehensively map cell lineages, we use single-cell RNA sequencing and antigen receptor analysis of almost half a million cells from up to 5 anatomical regions in the developing and up to 11 distinct anatomical regions in the healthy paediatric and adult human gut. This reveals the existence of transcriptionally distinct BEST4 epithelial cells throughout the human intestinal tract. Furthermore, we implicate IgG sensing as a function of intestinal tuft cells. We describe neural cell populations in the developing enteric nervous system, and predict cell-type-specific expression of genes associated with Hirschsprung's disease. Finally, using a systems approach, we identify key cell players that drive the formation of secondary lymphoid tissue in early human development. We show that these programs are adopted in inflammatory bowel disease to recruit and retain immune cells at the site of inflammation. This catalogue of intestinal cells will provide new insights into cellular programs in development, homeostasis and disease
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