Clinical-functional changes of myocardium after percutaneous coronary interventions in patients with chronic heart failure

The study aimed to evaluate the results of clinical and functional changes in the left ventricular myocardium in patients with CHF after transluminal balloon angioplasty (TLBAP) of the coronary arteries with stenting of the coronary arteries during the observed period and to study the safety of surgical intervention in these patients. The study showed a statistically significant decrease in the functional class of CHF after percutaneous coronary intervention, followed by an improvement in the diastolic function (E/A) of the LV in the course of the observed perio

Selection and synthesis of general mechanisms for formation of CHF in comorbid pathology

The work analyses the pathogenesis of chronic heart failure (CHF) with preserved ejection fraction (EF); General mechanisms of CHF formation in concomitant diseases are outlined; Modern data on the effect of co morbid pathology on cardiac remodelling and dysfunction in patients with chronic heart failure with a preserved EF are summarize

Post-covid musculo-articular syndrome and Covid-19 association with rheumatoid arthritis

The aim of the study was to analyze the current literature data on the prevalence of musculo-articular syndrome and to study the association of coronavirus infection with the development of rheumatoid arthritis.Цель работы – анализ современных литературных данных по распространенности мышечно-суставного синдрома и изучению связи перенесенной коронавирусной инфекции с развитием ревматоидного артрита

Harnessing the Technological Potential of Chia Seeds in the Technology of Cream-whipped Candy Masses

The technological properties of chia seeds have been studied. It has been established that the degree of their swelling depends on the type of a medium (water, albumin solution, fat) and the state of seeds (whole seeds or ground seeds). It was noted that the whole seeds have a higher capacity to retain water than the ability to retain an albumin solution or fat, by 1.87 and 17.28 times, and the ground seeds – by 1.75 and 17.49 times, respectively. Their capacity to swell improves after grinding regardless of the type of a medium. In addition, the ground seeds have a better fat-emulsifying ability but they do not demonstrate the foaming properties. The whole chia seeds have good foaming properties. It is possible to obtain a whipped protein mass, which is not worse than the control sample in terms of stability and foaming capacity, in case of replacing 40 % of dry albumin with whole chia seeds.We recommend adding the whole chia seeds at the stage of the whipping of protein mass, and the ground seeds – at the stage of obtaining a fat emulsion semi-finished product in the production of cream-whipped candy masses. Thus, the formulation amount of dry albumin and fat decreases. The addition of 30 % of whole seeds and 30 % of ground seeds helps reduce the density of structured cream-whipped candy mass by 6.7 %. A further increase in the dosage of the additive leads to a slight increase in the value of the density indicator. In addition, an increase in the content of chia seeds causes an increase in the strength indicator of samples. The organoleptic analysis showed that the structured cream-whipped candy masses with the most studied dosage of chia seeds have the densified structure, uneven porosity, and strong, viscous consistency. It was found that the dosage of whole seeds should equal 40 % by weight of egg albumin, and the dosage of ground seeds – 40 % by weight of fat to ensure the high quality of cream-whipped candy masses.The obtained results are of practical importance for improving the technology of cream-whipped candy masses towards decreasing the formulation amount of albumin and margarine. The addition of chia seeds would improve the nutritional and biological values of cream-whipped candie

Short-Term Effect of SARS-CoV-2 Spike Protein Receptor-Binding Domain-Specific Antibody Induction on Neutrophil-Mediated Immune Response in Mice

Vaccination protects against COVID-19 via the spike protein receptor-binding domain (RBD)-specific antibody formation, but it also affects the innate immunity. The effects of specific antibody induction on neutrophils that can cause severe respiratory inflammation are important, though not completely investigated. In the present study, using a mouse model mimicking SARS-CoV-2 virus particle inhalation, we investigated neutrophil phenotype and activity alterations in the presence of RBD-specific antibodies. Mice were immunized with RBD and a week after a strong antibody response establishment received 100 nm particles in the RBD solution. Control mice received injections of a phosphate buffer instead of RBD. We show that the application of 100 nm particles in the RBD solution elevates neutrophil recruitment to the blood and the airways of RBD-immunized mice rather than in control mice. Analysis of bone marrow cells of mice with induced RBD-specific antibodies revealed the increased population of CXCR2+CD101+ neutrophils. These neutrophils did not demonstrate an enhanced ability of neutrophil extracellular traps (NETs) formation compared to the neutrophils from control mice. Thus, the induction of RBD-specific antibodies stimulates the activation of mature neutrophils that react to RBD-coated particles without triggering excessive inflammation

Aspergillus fumigatus Infection-Induced Neutrophil Recruitment and Location in the Conducting Airway of Immunocompetent, Neutropenic, and Immunosuppressed Mice

Susceptibility to fungal infection is commonly associated with impaired neutrophil responses. To study the mechanisms underlying this association, we investigated neutrophil recruitment to the conducting airway wall after Aspergillus fumigatus conidium inhalation in mouse models of drug-induced immunosuppression and antibody-mediated neutrophil depletion (neutropenia) by performing three-dimensional confocal laser-scanning microscopy of whole-mount primary bronchus specimens. Actin staining enabled visualization of the epithelial and smooth muscle layers that mark the airway wall. Gr-1+ or Ly6G+ neutrophils located between the epithelium and smooth muscles were considered airway wall neutrophils. The number of airway wall neutrophils for immunocompetent, immunosuppressed, and neutropenic mice before and 6 h after A. fumigatus infection were analyzed and compared. Our results show that the number of conducting airway wall neutrophils in immunocompetent mice significantly increased upon inflammation, while a dramatic reduction in this number was observed following immunosuppression and neutropenia. Interestingly, a slight increase in the infiltration of neutrophils into the airway wall was detected as a result of infection, even in immunosuppressed and neutropenic mice. Taken together, these data indicate that neutrophils are present in intact conducting airway walls and the number elevates upon A. fumigatus infection. Conducting airway wall neutrophils are affected by both neutropenia and immunosuppression

Murine Intraepithelial Dendritic Cells Interact With Phagocytic Cells During Aspergillus fumigatus-Induced Inflammation

People are constantly exposed to airborne fungal spores, including Aspergillus fumigatus conidia that can cause life-threatening conditions in immunocompromised patients or acute exacerbations in allergics. However, immunocompetent hosts do not exhibit mycoses or systemic inflammation, due to the sufficient but not excessive antifungal immune response that prevent fungal invasion. Intraepithelial dendritic cells (IE-DCs) of the conducting airway mucosa are located in the primary site of the inhalant pathogen entry; these cells can sense A. fumigatus conidia and maintain homeostasis. The mechanisms by which IE-DCs contribute to regulating the antifungal immune response and controlling conidia dissemination are not understood. To clarify the role of IE-DCs in the balance between pathogen sensing and immune tolerance we investigated the A. fumigatus conidia distribution in optically cleared mouse lungs and estimated the kinetics of the local phagocytic response during the course of inflammation. MHCII+ antigen-presenting cells, including IE-DCs, and CD11b+ phagocytes were identified by immunohistochemistry and three-dimensional fluorescence confocal laser-scanning microscopy of conducting airway whole-mounts. Application of A. fumigatus conidia increased the number of CD11b+ phagocytes in the conducting airway mucosa and induced the trafficking of these cells through the conducting airway wall to the luminal side of the epithelium. Some CD11b+ phagocytes internalized conidia in the conducting airway lumen. During the migration through the airway wall, CD11b+ phagocytes formed clusters. Permanently located in the airway wall IE-DCs contacted both single CD11b+ phagocytes and clusters. Based on the spatiotemporal characteristics of the interactions between IE-DCs and CD11b+ phagocytes, we provide a novel anatomical rationale for the contribution of IE-DCs to controlling the excessive phagocyte-mediated immune response rather than participating in pathogen uptake