46 research outputs found

    Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM

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    The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers bridge the origin of transfer (oriT) to a key component of the conjugative pore, the coupling protein TraD. Here we show that TraM recognizes a high-affinity DNA-binding site, sbmA, as a cooperative dimer of tetramers. The crystal structure of the TraM–sbmA complex from the plasmid pED208 shows that binding cooperativity is mediated by DNA kinking and unwinding, without any direct contact between tetramers. Sequence-specific DNA recognition is carried out by TraM’s N-terminal ribbon–helix–helix (RHH) domains, which bind DNA in a staggered arrangement. We demonstrate that both DNA-binding specificity, as well as selective interactions between TraM and the C-terminal tail of its cognate TraD mediate conjugation specificity within the F-like family of plasmids. The ability of TraM to cooperatively bind DNA without interaction between tetramers leaves the C-terminal TraM tetramerization domains free to make multiple interactions with TraD, driving recruitment of the plasmid to the conjugative pore

    Surpassing Expectations: State of the U.S. Wind Power Market

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    Comparison of AEO 2010 Natural Gas Price Forecast to NYMEX Futures Prices

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    Making european-style community wind power development work in the United States

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    Once primarily a European phenomenon, community wind power development--defined here as one or more locally owned, utility-scale wind turbines interconnected on either the customer or utility side of the meter--is gaining a foothold in an increasing number of states throughout the United States. This article describes the various policies and incentives that Minnesota, Wisconsin, Iowa, and Massachusetts are using to support community wind power development, and how state and federal support influences the types of projects and ownership structures that are being developed. Experience in these states demonstrates that, with an array of incentives and creative financing schemes targeted at community-scale projects, there are opportunities to make community wind work in the United States
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