Hyperactivity In Boys With Attention-deficit/hyperactivity Disorder: A Ubiquitous Core Symptom Or Manifestation Of Working Memor

Hyperactivity is currently considered a core and ubiquitous feature of attention-deficit/hyperactivity disorder (ADHD); however, an alternative model challenges this premise and hypothesizes a functional relationship between working memory (WM) and activity level. The current study investigated whether children s activity level is functionally related to WM demands associated with the domain-general central executive and subsidiary storage/rehearsal components using tasks based on Baddeley s (2007) WM model. Activity level was objectively measured 16 times per second using wrist- and ankle-worn actigraphs while 23 boys between 8 and 12 years of age completed control tasks and visuospatial/phonological WM tasks of increasing memory demands. All children exhibited significantly higher activity rates under all WM relative to control conditions, and children with ADHD (n=12) moved significantly more than typically developing children (n=11) under all conditions. Activity level in all children was associated with central executive but not storage/rehearsal functioning, and higher activity rates exhibited by children with ADHD under control conditions were fully attenuated by removing variance directly related to central executive processes

Political Messaging Over Time: A Comparison of US Presidential Candidate Facebook Posts and Tweets in 2016 and 2020

Political campaigns have a temporal nature, which means that the strategic environment shapes the nature of candidate communication, especially the stages of campaigning—from surfacing to the general election. As social media platforms have matured and political campaigns have normalized their use of those platforms in this decade, this study examines the 2016 and 2020 US presidential campaign communication on Facebook and Twitter using data from the Illuminating project at Syracuse University. Our objective is to explore how the stages of the campaign cycle shape political communication. We also explore social media platforms as additional factors. Moreover, given the distinct and anti-normative communication style of Donald Trump, we examine whether his communication is an outlier relative to his competition in the primaries and the general election, and while a challenger in 2016 and an incumbent in 2020. Our results suggest that campaign messaging changes over the stages of the campaign, with candidates more likely to advocate for themselves during the crowded primaries, and then engage in high volumes of calls to action in the general election. The 2016 posts were substantially more attack-focused than in 2020. There is some evidence to suggest that the global pandemic affected the ways in which campaigns used their social media accounts. Of note, campaigns seem to heavily rely on Facebook for all types of strategic communication, even as the academic community primarily analyzes Twitter. Finally, Trump’s sum-total of his discourse is less negative than Clinton’s in 2016 and more advocacy-focused, overall

A Novel Epigenetic Phenotype Associated With the Most Aggressive Pathway of Bladder Tumor Progression

International audienceBackground: Epigenetic silencing can extend to whole chromosomal regions in cancer. There have been few genome-wide studies exploring its involvement in tumorigenesis.Methods: We searched for chromosomal regions affected by epigenetic silencing in cancer by using Affymetrix microarrays and real-time quantitative polymerase chain reaction to analyze RNA from 57 bladder tumors compared with normal urothelium. Epigenetic silencing was verified by gene re-expression following treatment of bladder cell lines with 5-aza-deoxycytidine, a DNA demethylating agent, and trichostatin A, a histone deacetylase inhibitor. DNA methylation was studied by bisulfite sequencing and histone methylation and acetylation by chromatin immunoprecipitation. Clustering was used to distinguish tumors with multiple regional epigenetic silencing (MRES) from those without and to analyze the association of this phenotype with histopathologic and molecular types of bladder cancer. The results were confirmed with a second panel of 40 tumor samples and extended in vitro with seven bladder cancer cell lines. All statistical tests were two-sided.Results: We identified seven chromosomal regions of contiguous genes that were silenced by an epigenetic mechanism. Epigenetic silencing was not associated with DNA methylation but was associated with histone H3K9 and H3K27 methylation and histone H3K9 hypoacetylation. All seven regions were concordantly silenced in a subgroup of 26 tumors, defining an MRES phenotype. MRES tumors exhibited a carcinoma in situ-associated gene expression signature (25 of 26 MRES tumors vs 0 of 31 non-MRES tumors, P < 10⁻¹⁴), rarely carried FGFR3 mutations (one of 26 vs 22 of 31 non-MRES tumors, P < 10⁻¹⁶), and contained 25 of 33 (76%) of the muscle-invasive tumors. Cell lines derived from aggressive bladder tumors presented epigenetic silencing of the same regions.Conclusions: We have identified an MRES phenotype characterized by the concomitant epigenetic silencing of several chromosomal regions, which, in bladder cancer, is specifically associated with the carcinoma in situ gene expression signature

Understanding Phonological Memory Deficits In Boys With Attention-deficit/hyperactivity Disorder (adhd): Dissociation Of Short-term Storage And Articulatory Rehearsal Processes

The current study dissociated and examined the two primary components of the phonological working memory subsystem – the short-term store and articulatory rehearsal mechanism – in boys with ADHD (n = 18) relative to typically developing boys (n = 15). Word lists of increasing length (2, 4, and 6 words per trial) were presented to and recalled by children following a brief (3 s) interval to assess their phonological short-term storage capacity. Children’s ability to utilize the articulatory rehearsal mechanism to actively maintain information in the phonological short-term store was assessed using word lists at their established memory span but with extended rehearsal times (12 s and 21 s delays). Results indicate that both phonological short-term storage capacity and articulatory rehearsal are impaired or underdeveloped to a significant extent in boys with ADHD relative to typically developing boys, even after controlling for age, SES, IQ, reading ability, and reading speed. Larger magnitude deficits, however, were apparent in short-term storage capacity (ES = 1.15 to 1.98) relative to articulatory rehearsal (ES = 0.47 to 1.02). These findings are consistent with previous reports of deficient phonological short-term memory in boys with ADHD, and suggest that future attempts to develop remedial cognitive interventions for children with ADHD will need to include active components that require children to hold increasingly more information over longer time interval

DOES ANYONE TALK ABOUT THE ISSUES ANYMORE? THE 2016 U.S. PRESIDENTIAL CANDIDATES' MESSAGING ON FACEBOOK AND TWITTER

As campaigns use social media to communicate with the public, this study investigates the dynamics of issue and image construction by the U.S. presidential candidates during the primary stage of the 2016 presidential campaign. Using computational techniques to classify candidate posts by message type and topic, we study all posts by the 17 Republican and 5 Democratic candidates from Sept. 1, 2015 through March 31, 2016. We ask whether candidates post more about their image--their character and personality--or on issues, and when they post on issues, does each candidate own specific policy issues. We also investigate which topics the public tends to engage with more. We also ask if there are differences in how the candidates use social media. Our results suggest that candidates post substantially less on the issues as compared with other types of messages. When they do post about policy topics, the candidates are associated with distinct policy positions, suggesting issue ownership as a strategic differentiator. Results also suggest campaigns use Facebook in ways different from Twitter, further reinforcing prior scholarship suggesting that campaigns use their social media for different purposes given different audiences on the platforms. Our findings indicate that campaigns overall are not discussing policy matters, thereby depriving the public the opportunity to engage in discussion of vital issues and what they would do to solve them; instead, the cult of personality seems to be further exacerbated by social media

Phylogenomics of nontuberculous mycobacteria respiratory infections in people with cystic fibrosis.

Nontuberculous mycobacteria (NTM) can cause severe pulmonary disease in people with cystic fibrosis (pwCF). These infections present unique challenges for diagnosis and treatment, prompting a recent interest in understanding NTM transmission and pathogenesis during chronic infection. Major gaps remain in our knowledge regarding basic pathogenesis, immune evasion strategies, population dynamics, recombination potential, and the evolutionary implications of host and antibiotic pressures of long-term NTM infections in pwCF. Phylogenomic techniques have emerged as an important tool for tracking global patterns of transmission and are beginning to be used to ask fundamental biological questions about adaptation to the host during pathogenesis. In this review, we discuss the burden of NTM lung disease (NTM-LD), highlight the use of phylogenomics in NTM research, and address the clinical implications associated with these studies

Screening For High-Risk Drinking In A College Student Health Center: Characterizing Students Based On Quantity, Frequency, And Harms.

OBJECTIVE: This study examined characteristics of students who presented to a college health center and screened positive for the 5/4 definition of high-risk drinking (five or more drinks in a row for men, or four or more drinks in a row for women, on at least one occasion in the past 2 weeks) and analyzed the students\u27 data according to their reporting of alcohol-related harms. METHOD: Secondary analysis of data obtained for an intervention study to reduce high-risk drinking in college students was used. Data on alcohol use and alcohol-related harms were obtained from Web-based Healthy Lifestyle Questionnaires and 30-day alcohol recall diaries (Timeline Followback calendar). Students (N = 363; 52% female) were classified as nonheavy, heavy, and heavy and frequent drinkers, based on their self-reported alcohol use. Alcohol-related harms were measured using the Rutgers Alcohol Problem Index and eight additional items derived from the Drinker Inventory of Consequences-2L. RESULTS: Students in the nonheavy, heavy, and heavy and frequent groups had mean Rutgers Alcohol Problem Index scores of 10, 14, and 23, respectively. The heavy-and-frequent drinking group comprised 20% of the sample but experienced 31% of the total harms. CONCLUSIONS: The 5/4 screening question accurately identified college students presenting to a college health center who were already experiencing significant alcohol-related harms. The addition of a frequency question (drinking 3 or more days per week) to the 5/4 screening question provided a simple method for identifying those students at highest risk and in greatest need of intervention