Matrix-assisted laser desorption/ionization time of flight mass spectrometry identification of Vibrio (Listonella) anguillarum isolated from sea bass and sea bream

Vibrio (Listonella) anguillarum is a pathogenic bacterium causing septicaemia in a wide range of marine organisms and inducing severe mortalities, thus it is crucial to conduct its accurate and rapid identification. The aim of this study was to assess MALDI-TOF MS as a method of choice for identification of clinical V. anguillarum isolates from affected marine fish. Since the method accuracy might be influenced by the type of the medium used, as well as by the incubation conditions, we tested V. anguillarum isolates grown on standard media with and without the addition of NaCl, cultured at three incubation temperatures, and at three incubation periods. The best scores were retrieved for V. anguillarum strains grown on NaCl-supplemented tryptone soy agar (TSA) at 22°C and incubated for 48h (100% identification to species level; overall score 2.232), followed by incubation at 37°C and 48h (100% to species level; score 2.192). The strains grown on non-supplemented TSA gave the best readings when incubated at 22°C for 72h (100% identification to species level; overall score 2.182), followed by incubation at 15°C for 72h (100% to species level; score 2.160). Unreliable identifications and no-identifications were growing with the incubation duration at 37°C, on both media, amounting to 88.89% for 7d incubation on supplemented TSA, and 92.60% for 7d incubation on non-supplemented TSA. The age of the cultured strains and use of media significantly impacted the mass spectra, demonstrating that for reliable identification, MALDI-TOF MS protein fingerprinting with the on-target extraction should be performed on strains grown on a NaCl-supplemented medium at temperatures between 15 and 22°C, incubated for 48-72 hours

How service design cues help in service failures

Terres, M. D. S., Herter, M. M., Pinto, D. C., & Mazzon, J. A. (2020). The power of sophistication: How service design cues help in service failures. Journal of Consumer Behaviour, 19(3), 277-290. https://doi.org/10.1002/cb.1816By analyzing three experimental studies, this research tests how and when sophisticated service environment designs (compared to modest service designs) can minimize consumers' negative emotions and increase repurchase intentions after a failure. Drawing on part-list cueing literature, this research proposes that when a service failure occurs in a sophisticated (vs. modest) environment, consumers will rely on the sophisticated style of design as cues for service quality. We argue that sophisticated (vs. modest) service designs work as strong cues for quality that restrict the retrieval of negative information by consumers and can minimize the negative impacts of service failure, reducing consumers' negative emotions and increasing repurchase intentions. We further advance our theorizing by showing how choice failure consequences (i.e., the risk or consequence related to the service choice) moderate the effects via associative pathways of retrieval. The findings contribute to theory and practice by revealing how service designs can serve as cues to mitigate adverse consequences of service failure.authorsversionpublishe

MODELO DE GESTÃO DE EGRESSOS PARA AS FACULDADES INTEGRADAS PROMOVE DE BRASÍLIA: UM ESTUDO DE CASO

As demandas atuais na prestação de serviços de educação exigem que as Instituições de Ensino Superior apresentem critérios de gestão bem definidos. Neste contexto, o presente estudo tem por objetivo desenvolver um modelo de gestão de egressos aplicável às Faculdades Integradas Promove de Brasília. Foi realizado um levantamento de dados por meio de pesquisa bibliográfica gerando um Modelo de Gestão com instrumentos de execução para cada uma de suas etapas. Em seguida, um Grupo de Trabalho (GT) formado por alunos egressos avaliou o modelo elaborado com base em sua realidade acadêmica. Os resultados demonstraram que a implantação de um modelo de gestão de egressos é de suma importância para a realidade da IES, e que as proposições da teoria e modelos de gestão já aplicados em outras instituições pesquisadas refletem a realidade do ambiente deste estudo. Por fim, observou-se que a implantação de um modelo de gestão de egressos proporciona a IES uma interação contínua com seu alunado. Palavras-chaves: Gestão de Pessoas. Egressos. Instituição de Ensino Superior

Strength and thermal stability of Cu-Al2O3 composite obtained by internal oxidation

The objective of the work is to study the effects of the high-energy milling on strengthening, thermal stability and electrical conductivity of Cu-Al2O3 composite. The prealloyed copper powders, atomized in inert gas and containing 3 wt. % Al, were milled up to 20 h in the planetary ball mill to oxidize in situ aluminium with oxygen from the air. Composite compacts were obtained by hot-pressing in an argon atmosphere at 800 degrees C for 3 h under the pressure of 35 MPa. The microstructural characterization was performed by the optical microscope, scanning electron microscope (SEM), transmission electron microscope (TEM) and X-ray diffraction analysis (XRD). The microhardness, electrical conductivity and density measurements were also carried out. The effect of internal oxidation and high-energy milling on strengthening of Cu-Al2O3 composite was significant, The increase of the microhardness of composite compacts (292 HV) is almost threefold comparing to compacts processed from the as-received Cu-3 wt. % Al powder (102 HV). The grain size of Cu-Al2O3 compacts processed from 5 and 20 h-milled powders was 75 and 45 nm, respectively. The small increase in the grain size and the small microhardness drop indicate the high thermal stability of Cu-Al2O3 composite during high-temperature exposure at 800 degrees C

Anesthesia and Patients with Congenital Hyposensitivity to Pain

Background: Congenital hyposensitivity to pain or hereditary sensory and autonomic neuropathy represents a variety of disorders characterized by decreased perception of nociception, loss of other modalities of sensation, and variable expression of autonomic dysfunction. Sensory loss, especially that of pain, is associated with self-mutilations that may require frequent operations. Little is known about the safety of anesthesia for these patients. Methods: The authors performed a computerized search of the Mayo Clinic medical records database between January 1996 and November 2005 for patients with congenital hyposensitivity to pain and related disorders who underwent general anesthesia. Medical records were reviewed for demographics, anesthetic techniques and agents, use of opioids, and perioperative complications. In addition, the authors conducted a comprehensive review of the literature to summarize the current knowledge regarding anesthesia for patients with congenital hyposensitivity to pain, and compared it with the patients with hyposensitivity to pain identified at the Mayo Clinic. Results: The authors identified seven patients with hereditary sensory and autonomic neuropathy II, IV, or V and undefined variants of congenital pain hyposensitivity who generated 17 anesthesia records: 12 for orthopedic operations, 3 for sural nerve biopsies, and 2 for ophthalmologic procedures. In all patients, standard doses of volatile agents were used during anesthesia. Small amounts of opioids were used during the course of eight operations. Most patients experienced mild hypothermia (lowest temperature 34.7°C), and none experienced hyperthermia. All patients were hemodynamically stable during otherwise uneventful anesthesia. During recovery from anesthesia, opioids were given to only one patient, a single dose of 1 mg morphine. Even after major orthopedic operations, the patient did not require additional analgesia. Conclusions: The patients with profound congenital hyposensitivity to pain underwent anesthesia without any adverse events. The authors found that despite reduced pain perception, the requirements for volatile anesthetics were within the expected range for population with normal pain perception, but they did not require opioids postoperatively. Intraoperative mild hypothermia was easily managed by adjustment of environmental temperature

Using Soil and Water Conservation Contests for Extension: Experiences from the Bolivian Mountain Valleys

Soil and water conservation (SWC) contests among farmer groups were organized in five rural villages in the Bolivian mountain valleys. The contests were aimed at quickly achieving widespread sustainable results. This article analyzes the effectiveness of these contests as an extension tool. Mixed results were obtained. In three villages, participation rates in the SWC activities introduced in the contests were still high even 2 years after project withdrawal. These were all villages where a solid foundation for sustainable development had been laid before the contests were held. Two years later, most families were still involved in maintenance of the SWC practices introduced in the contests, and many farmers had started to experiment with different soil management practices. However, replications of these SWC practices were not widespread, Conservation Leaders did not continue with their training activities, and the quality of maintenance of the practices was often not satisfactory. In order to become a more effective extension tool and achieve widespread impact, SWC contests must receive continued support by a catalyst agency. Moreover, other SWC contests should also be organized in which practices are not predefined. Given that SWC contests are a low-budget extension tool, local municipalities could become more actively involved

Proceso agrario en Bolivia y América Latina

El material esta distribuido en cinco partes. La primera está referida a los resultados y perspectivas de la Reforma Agraria en Bolivia. Está dividida en tres temáticas: la Reforma Agraria en las Tierras Altas, la Reforma Agraria en las Tierras Bajas y la Reforma Agraria en el Chaco. La segunda parte contiene las intervenciones realizadas por los invitados extranjeros sobre los resultados y perspectivas de la Reforma Agraria en América Latina y en México, Perú y Ecuador. La tercera parte corresponde a las intervenciones de los representantes de las organizaciones indígenas y campesinas bolivianas, que fueron invitados para debatir el tema Tierra, Democracia y Poder. Esta parte esta precedida por un recuento general de la situación de los Movimientos Indígenas-Campesinos en América Latina y sus perspectivas. La cuarta da cuenta de las intervenciones hechas por los representantes de las organizaciones de productores agrarios, campesinos y empresarios, quienes fueron invitados para debatir el tema Tierra, Economía e Integración. La última parte es una síntesis de las ideas y aspectos relevantes producto de las exposiciones y de las preguntas de los asistentes al seminario, que dejaron una estela de inquietudes e iniciativas que son el eslabón para futuros debates en otros espacios de reflexión en diferentes lugares del país, con la participación de actores y pensadores, operadores y promotores del desarrollo rural.Presentación 7; Introducción 8; La Reforma Agraria abandonada: valles y altiplano, Miguel Urioste F. de C. 19; Medio siglo de Reforma Agraria boliviana, Danilo Paz Ballivián 53; La tierra es de quien la trabaja, René Salomón Vargas 65; La Reforma Agraria en las tierras bajas de Bolivia, Carlos Romero Bonifaz 83; Lógicas de ocupación territorial en la agricultura y la forestería empresarial en las tierras bajas, Alan Bojanic 127; Antecedentes, situación actual y recomendaciones en la tenencia de la tierra en el oriente boliviano, Cámara Agropecuaria del Oriente 137; 50 años de la Reforma Agraria en el Chaco boliviano, Oscar Bazoberry Chali 145; Resultados de los cambios introducidos por la Reforma Agraria, Erwin Galoppo von Borries 177; La Reforma Agraria en Bolivia y el MST, Ermelinda Fernández Bamba 191; La Reforma Agraria en América Latina, Jacques Chonchol 205; De rústicas revueltas: Añoranza y utopía en el México rural, Armando Bartra 223; Reforma y contrarreforma agraria en el Perú, Laureano del Castillo Pinto 255; Reforma Agraria en el Ecuador, Fausto Jordán B. 285; 50 años después, emergencia étnica, Xavier Albó 321; Tierra, mujer y desarrollo, María Machaca 329; Tierra y territorio comunitario, Bienvenido Zacu 333; Tierra, migración y colonización, José Luis Méndez Chaurara 341; Tierra, coca y desarrollo, Dionisio Núñez 355; Tierra para todos, Ángel Durán 359; Tierra y producción orgánica, Mario R. Cordero Camacho 367; Tierra y producción campesina, María Julia Jiménez 377; Tierra y Territorio y Políticas para la Tierra, Elizabeth Jiménez Zamora 385; Ideas y aspectos relevantes del Seminario, John D.Vargas Vega 429