Air quality monitoring using a whole cell based sensor system

AbstractIn this work, a novel whole-cell based biosensor for the detection of toxic substances in air was established. Due to their basic necessity of liquid environment, cell-based biosensors were underrepresented in the field of gas sensors in the last decades. The adaption of a commercial sensor chip (Bionas®) for the measurement of pollutants in liquids enables the direct exposure of cells with air. Cells of the respiration tract (A549, RPMI2650, V79), which tend to survive at a gas phase, are used as biological receptors. Three physiological cell parameters are monitored continuously in parallel (acidification, respiration, morphology). Water insoluble gases (e.g. CO) as well as water soluble gases (e.g. NH3) were used as model gases to test the feasibility of the novel sensor system. MIR- measurements proofed the reproducibility of the draining method. This sensor system provides a basis for many sensing applications such as environmental monitoring, building technology and public security

Monitoring of irritant gas using a whole-cell-based sensor system

Cell-based sensors for the detection of gases have long been underrepresented, due to the cellular requirement of being cultured in a liquid environment. In this work we established a cell-based gas biosensor for the detection of toxic substances in air, by adapting a commercial sensor chip (Bionas ® ), previously used for the measurement of pollutants in liquids. Cells of the respiratory tract (A549, RPMI 2650, V79), which survive at a gas phase in a natural context, are used as biological receptors. The physiological cell parameters acidification, respiration and morphology are continuously monitored in parallel. Ammonia was used as a highly water-soluble model gas to test the feasibility of the sensor system. Infrared measurements confirmed the sufficiency of the medium draining method. This sensor system provides a basis for many sensor applications such as environmental monitoring, building technology and public security. © 2012 Elsevier B.V. All rights reserved.status: publishe

Air Quality Monitoring using a Whole-Cell based Sensor System

In this work, a novel whole-cell based biosensor for the detection of toxic substances in air was established. Due to their basic necessity of liquid environment, cell-based biosensors were underrepresented in the field of gas sensors in the last decades. The adaption of a commercial sensor chip (Bionas®) for the measurement of pollutants in liquids enables the direct exposure of cells with air. Cells of the respiration tract (A549, RPMI2650, V79), which tend to survive at a gas phase, are used as biological receptors. Three physiological cell parameters are monitored continuously in parallel (acidification, respiration, morphology). Water insoluble gases (e.g. CO) as well as water soluble gases (e.g. NH3) were used as model gases to test the feasibility of the novel sensor system. MIR-measurements proofed the reproducibility of the draining method. This sensor system provides a basis for many sensing applications such as environmental monitoring, building technology and public security. © 2011 Published by Elsevier Ltd.status: publishe