Pre-Figurative Structures for Social Connection

The world has many structures that foster social connection. Especially in the age of the internet, there are many off and online worlds that do so. Alternative festivals, temporary communities and electoral guerrilla theater organized online and practiced offline are all potential ways to prefigure the world we want to live in. Together, the FEAST team created physically and socially intelligent structures that facilitate cooperation, emotional release and transcend the expectations of architecture and infrastructure as fixed, emboldening viewers to become participants.http://deepblue.lib.umich.edu/bitstream/2027.42/169558/1/Honors_Capstone_Socially_Connected_Structures.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/169558/2/Honors_Capstone_Socially_Connected_Structures.ppt

Semisynthetic Nanoreactor for Reversible Single-Molecule Covalent Chemistry

Protein engineering has been used to remodel pores for applications in biotechnology. For example, the heptameric alpha-hemolysin pore (alpha HL) has been engineered to form a nanoreactor to study covalent chemistry at the single -molecule level. Previous work has been confined largely to the chemistry of cysteine side chains or, in one instance, to an irreversible reaction of an unnatural amino acid side chain bearing a terminal alkyne. Here, we present four different alpha HL pores obtained by coupling either two or three fragments by native chemical ligation (NCL). The synthetic alpha HL monomers were folded and incorporated into heptameric pores. The functionality of the pores was validated by hemolysis assays and by single-channel current recording. By using NCL to introduce a ketone amino acid, the nanoreactor approach was extended to an investigation of reversible covalent chemistry on an unnatural side chain at the single -molecule level

The Development of a Smart Magnetic Resonance Imaging and Chemical Exchange Saturation Transfer Contrast Agent for the Imaging of Sulfatase Activity

The molecular imaging of biomarkers plays an increasing role in medical diagnostics. In particular, the imaging of enzyme activity is a promising approach, as it enables the use of its inherent catalytic activity for the amplification of an imaging signal. The increased activity of a sulfatase enzyme has been observed in several types of cancers. We describe the development and in vitro evaluation of molecular imaging agents that allow for the detection of sulfatase activity using the whole-body, non-invasive MRI and CEST imaging methods. This approach relies on a responsive ligand that features a sulfate ester moiety, which upon sulfatase-catalyzed hydrolysis undergoes an elimination process that changes the functional group, coordinating with the metal ion. When Gd3+ is used as the metal, the complex can be used for MRI, showing a 25% decrease at 0.23T and a 42% decrease at 4.7T in magnetic relaxivity after enzymatic conversion, thus providing a "switch-off" contrast agent. Conversely, the use of Yb3+ as the metal leads to a "switch-on" effect in the CEST imaging of sulfatase activity. Altogether, the results presented here provide a molecular basis and a proof-of-principle for the magnetic imaging of the activity of a key cancer biomarker. </p

Momentum and Energy Distributions of Nucleons in Finite Nuclei due to Short-Range Correlations

The influence of short-range correlations on the momentum and energy distribution of nucleons in nuclei is evaluated assuming a realistic meson-exchange potential for the nucleon-nucleon interaction. Using the Green-function approach the calculations are performed directly for the finite nucleus $^{16}$O avoiding the local density approximation and its reference to studies of infinite nuclear matter. The nucleon-nucleon correlations induced by the short-range and tensor components of the interaction yield an enhancement of the momentum distribution at high momenta as compared to the Hartree-Fock description. These high-momentum components should be observed mainly in nucleon knockout reactions like $(e,e'p)$ leaving the final nucleus in a state of high excitation energy. Our analysis also demonstrates that non-negligible contributions to the momentum distribution should be found in partial waves which are unoccupied in the simple shell-model. The treatment of correlations beyond the Brueckner-Hartree-Fock approximation also yields an improvement for the calculated ground-state properties.Comment: 12 pages RevTeX, 7 figures postscript files appende

Generator Coordinate Calculations for the Breathing-Mode Giant Monopole Resonance in Relativistic Mean Field Theory

The breathing-mode giant monopole resonance (GMR) is studied within the framework of the relativistic mean-field theory using the Generator Coordinate Method (GCM). The constrained incompressibility and the excitation energy of isoscalar giant monopole states are obtained for finite nuclei with various sets of Lagrangian parameters. A comparison is made with the results of nonrelativistic constrained Skyrme Hartree-Fock calculations and with those from Skyrme RPA calculations. In the RMF theory the GCM calculations give a transition density for the breathing mode, which resembles much that obtained from the Skyrme HF+RPA approach and also that from the scaling mode of the GMR. From the systematic study of the breathing-mode as a function of the incompressibility in GCM, it is shown that the GCM succeeds in describing the GMR energies in nuclei and that the empirical breathing-mode energies of heavy nuclei can be reproduced by forces with an incompressibility close to $K = 300$ MeV in the RMF theory.Comment: 27 pages (Revtex) and 5 figures (available upon request), Preprint MPA-793 (March 1994

Will invertebrates require increasingly carbon-rich food in a warming world?

Elevated temperature causes metabolism and respiration to increase in poikilothermic organisms. We hypothesized that invertebrate consumers will therefore require increasingly carbon-rich diets in a warming environment because the increased energetic demands are primarily met using compounds rich in carbon, that is, carbohydrates and lipids. Here, we test this hypothesis using a new stoichiometric model that has carbon (C) and nitrogen (N) as currencies. Model predictions did not support the hypothesis, indicating instead that the nutritional requirements of invertebrates, at least in terms of food quality expressed as C∶N ratio, may change little, if at all, at elevated temperature. Two factors contribute to this conclusion. First, invertebrates facing limitation by nutrient elements such as N have, by default, excess C in their food that can be used to meet the increased demand for energy in a warming environment, without recourse to extra dietary C. Second, increased feeding at elevated temperature compensates for the extra demands of metabolism to the extent that, when metabolism and intake scale equally with temperature (have the same Q10), the relative requirement for dietary C and N remains unaltered. Our analysis demonstrates that future climate-driven increases in the C∶N ratios of autotroph biomass will likely exacerbate the stoichiometric mismatch between nutrient-limited invertebrate grazers and their food, with important consequences for C sequestration and nutrient cycling in ecosystems

Gauged motion in general relativity and in Kaluza-Klein theories

In a recent paper [1] a new generalization of the Killing motion, the {\it gauged motion}, has been introduced for stationary spacetimes where it was shown that the physical symmetries of such spacetimes are well described through this new symmetry. In this article after a more detailed study in the stationary case we present the definition of gauged motion for general spacetimes. The definition is based on the gauged Lie derivative induced by a threading family of observers and the relevant reparametrization invariance. We also extend the gauged motion to the case of Kaluza-Klein theories.Comment: 42 pages, revised version, typos correction along with some minor changes, Revtex forma

The Galactic Population of Low- and Intermediate-Mass X-ray Binaries

(abridged) We present the first study that combines binary population synthesis in the Galactic disk and detailed evolutionary calculations of low- and intermediate-mass X-ray binaries (L/IMXBs). We show that the formation probability of IMXBs with initial donor masses of 1.5--4 Msun is typically >~5 times higher than that of standard LMXBs, and suggest that the majority of the observed systems may have descended from IMXBs. Distributions at the current epoch of the orbital periods, donor masses, and mass accretion rates have been computed, as have orbital-period distributions of BMPs. Several significant discrepancies between the theoretical and observed distributions are discussed. The orbital-period distribution of observed BMPs strongly favors cases where the envelope of the neutron-star progenitor is more easily ejected during the common-envelope phase. However, this leads to a >~100-fold overproduction of the theoretical number of luminous X-ray sources relative to the total observed number of LMXBs. X-ray irradiation of the donor star may result in a dramatic reduction in the X-ray active lifetime of L/IMXBs, thus possibly resolving the overproduction problem, as well as the long-standing BMP/LMXB birthrate problem.Comment: 12 pages, emulateapj, submitted to Ap

Combining left atrial appendage closure and catheter ablation for atrial fibrillation: 2-year outcomes from a multinational registry

AIMS: Clinical practice guidelines do not recommend discontinuation of long-term oral anticoagulation in patients with a high stroke risk after catheter ablation for atrial fibrillation (AF). Left atrial appendage closure (LAAC) with Watchman has emerged as an alternative to long-term anticoagulation for patients accepting of the procedural risks. We report on the long-term outcomes of combining catheter ablation procedures for AF and LAAC from multicentre registries. METHODS AND RESULTS: Data were pooled from two prospective, real-world