118 research outputs found
The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
Most patients with acute suppurative meningitis are otherwise healthy
individuals with regard to immune mechanisms against invasive bacterial
disease. This medical emergency is among the most dramatic and
potentially ravaging diseases that affect humans, particularly young
children. The illness often strikes suddenly, and can either result in
death or leave the survivors with significant neurological
dysfunctions. The demonstration of a bacterial aetiology is necessary
for decisions regarding treatment and prophylaxis. Conventional
bacteriological methods frequently fail to identify an agent, as a
result of administration of antibiotics or delayed lumbar punctures. We
investigated the major aetiologic sources of unspecified bacterial
meningitis cases (G00.9, ISCD-10) by polymerase chain reaction
(PCR)-based identification of Neisseria meningitidis (crgA),
Streptococcus pneumoniae (ply) and Haemophilus influenzae (bexA) in
cerebrospinal fluid samples. The multiplex PCR detected N. meningitidis
in 92%, S. pneumoniae in 4% and H. influenzae in 1% of the 192 clinical
samples assayed; 3% were negative for all three DNA targets. Bacterial
DNA detection was found to be a valuable adjunct to enhance bacterial
meningitis surveillance when the yield of specimens by culture is
reduced. The implementation of PCR assays as a diagnostic procedure in
Public Health Laboratories is perceived to be a significant advance in
the investigation of bacterial meningitis
Enterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil
The presence of enterotoxigenic Bacteroides fragilis and nontoxigenic
B. fragilis (NTBF) among 109 strains isolated from 1980-2008 in Brazil
were investigated by PCR. One strain, representing 0.9% of the total
analyzed strains, harbored the bft gene which was identified as bft-1
isoform based on PCR-RFLP and sequencing. Forty-nine strains (44.9%)
exhibited the NTBF pattern III which possesses the flanking region
required for pathogenicity island acquisition in which the bftgene is
codified. These data reinforce the potential of B. fragilis as an
emerging enteropathogen in our country
Enterotoxigenic and nontoxigenic Bacteroides fragilis strains isolated in Brazil
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Previous issue date: 2008Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Mèdica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Mèdica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Mèdica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instittuto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Farmácia. Departamento de Tecnologia Farmacêutica. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Biologia de Anaeróbios. Rio de Janeiro, RJ, Brasil.The presence of enterotoxigenic Bacteroides fragilis and nontoxigenic B. fragilis (NTBF) among 109 strains
isolated from 1980-2008 in Brazil were investigated by PCR. One strain, representing 0.9% of the total analyzed
strains, harbored the bft gene which was identified as bft-1 isoform based on PCR-RFLP and sequencing. Fortynine
strains (44.9%) exhibited the NTBF pattern III which possesses the flanking region required for pathogenicity
island acquisition in which the bft gene is codified. These data reinforce the potential of B. fragilis as an emerging
enteropathogen in our country
Assessment of a two-step nucleic acid amplification assay for detection of Neisseria meningitidis followed by capsular genogrouping
Immediate prevention of meningococcal disease relies in part on the prompt treatment with antibiotics of household and other close contacts of cases; however intervention with effective vaccination relies on identification of serogroup-causing strains. Parenteral antibiotic for patient with suspected meningococcal disease before hospital admission is currently recommended. Laboratory standard methods are hindered by failure to detect bacteria by this medical approach to improve patient prognosis. We assessed two polymerase chain reaction (PCR) assays to detect (crgA) and define the serogroups (siaD, orf-2, and ctrA) of Neisseria meningitidis in 120 cerebrospinal fluid (CSF) samples from positive cases (culture or antigen detection or direct smear). The PCR sensitivity for the identification of N. meningitidis was 100% (95% confidence interval, CI, 96-100%) compared to a sensitivity of 46% for culture (95% CI 37-55%), 61% for latex agglutination test (95% CI 52-70%), and 68% for Gram stain (95% CI 59-76%); PCR specificity was 97% (95% CI 82-100%). PCR correctly identified the serogroups A, B, C, W135, Y, and X in CSF samples with a sensitivity of 88% (95% CI 80-93%); the primer sets were 100% specific. The introduction of PCR-based assays shall increase laboratory confirmed cases, consequently enhancing surveillance of meningococcal disease
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