1992 Archaeological Excavations at the Retallick-Brewer House site in Annapolis, Maryland, 18AP37

This report provides a detailed summary of the archaeological excavations that were conducted over a period of 2 weeks at the Retallick-Brewer House site located in Annapolis, Maryland. The project was initiated by the Griffis Foundation in order to gain some insight into the changes that have occurred at this property over its 200 year occupation. The project was completed by staff and volunteers of Archaeology In Annapolis, a joint venture of the University of Maryland, College Park, and Historic Annapolis Foundation. The design of this report follows the "Guidelines for Archaeological Investigations in Maryland" (McNamara 1981). This report contains descriptive summaries of individual levels along with an interpretation for each excavated unit in order to allow archaeologists and interested others access to the information contained within

Ampelometric evaluation of wild grape (Vitis vinifera L. ssp. sylvestris (C.C. Gmel.) Hegi) accessions in the germplasm collection of FEM-IASMA, Italy

In this paper, 45 wild grapevine accessions collected during two consecutive years were compared for 36 ampelometric traits using digital image analysis. The sample set contained male and female individuals from different geographic regions: Germany, North Italy, Central Italy, South Italy, Sardinia and Turkey. The leaf morphological data from the collected samples suggest that geographic origin, gender and vintage could have an effect on ampelometric traits in this species

GRA.LE.D. (GRApevine LEaf Digitalization) Software for the Detection and Graphic Reconstruction of Ampelometric Differences Between Vitis Leaves

Raster graphic ampelometric software was not exclusively developed for the estimation of leaf area,but also for the characterization of grapevine (Viti vinifera L.) leaves. The software was written in C++programming language, using the C++ Builder 2007 for Windows 95-XP and Linux operation systems. Ithandles desktop-scanned images. On the image analysed with the GRA.LE.D., the user has to determine11 points. These points are then connected and the distances between them calculated. The GRA.LE.D.software supports standard ampelometric measurements such as leaf area, angles between the veins andlengths of the veins. These measurements are recorded by the software and exported into plain ASCII textfiles for single or multiple samples. Twenty-two biometric data points of each leaf are identified by theGRA.LE.D. It presents the opportunity to statistically analyse experimental data, allows comparison ofcultivars and enables graphic reconstruction of leaves using the Microsoft Excel Chart Wizard. The GRA.LE.D. was thoroughly calibrated and compared to other widely used instruments and methods such asphoto-gravimetry, LiCor Li3100, WinDIAS2.0 and ImageTool. By comparison, the GRA.LE.D. presentedthe most accurate measurements of leaf area, but the LiCor Li3100 and the WinDIAS2.0 were faster, whilethe photo-gravimetric method proved to be the most time-consuming. The WinDIAS2.0 instrument wasthe least reliable. The GRA.LE.D. is uncomplicated, user-friendly, accurate, consistent, reliable and haswide practical application

Stability of Ampelometric Characteristics of Vitis vinifera L. cv. ‘Syrah’ and ‘Sauvignon blanc’ Leaves: Impact of Within-vineyard Variability and Pruning Method/Bud Load

Historically, grapevine (Vitis vinifera L.) leaf characterisation has been a driving force in the identificationof cultivars. In this study, ampelometric (foliometric) analysis was done on leaf samples collected fromhand-pruned, mechanically pruned and minimally pruned ‘Sauvignon blanc’ and ‘Syrah’ vines to estimatethe impact of within-vineyard variability and a change in bud load on the stability of leaf properties.The results showed that within-vineyard variability of ampelometric characteristics was high within acultivar, irrespective of bud load. In terms of the O.I.V. coding system, zero to four class differences wereobserved between minimum and maximum values of each characteristic. The value of variability of eachcharacteristic was different between the three levels of bud load and the two cultivars. With respect to budload, the number of shoots per vine had a significant effect on the characteristics of the leaf laminae. Singleleaf area and lengths of veins changed significantly for both cultivars, irrespective of treatment, while anglebetween veins proved to be a stable characteristic. A large number of biometric data can be recorded on asingle leaf; the data measured on several leaves, however, are not necessarily unique for a specific cultivar.The leaf characteristics analysed in this study can be divided into two groups according to the response toa change in bud load, i.e. stable (angles between the veins, depths of sinuses) and variable (length of theveins, length of the petiole, single leaf area). The variable characteristics are not recommended to be usedin cultivar identification, unless the pruning method/bud load is known

Assembly in G1 phase and long-term stability are unique intrinsic features of CENP-A nucleosomes

Centromeres are the site of kinetochore formation during mitosis. Centromere protein A (CENP-A), the centromere-specific histone H3 variant, is essential for the epigenetic maintenance of centromere position. Previously we showed that newly synthesized CENP-A is targeted to centromeres exclusively during early G1 phase and is subsequently maintained across mitotic divisions. Using SNAP-based fluorescent pulse labeling, we now demonstrate that cell cycle-restricted chromatin assembly at centromeres is unique to CENP-A nucleosomes and does not involve assembly of other H3 variants. Strikingly, stable retention is restricted to the CENP-A/H4 core of the nucleosome, which we find to outlast general chromatin across several cell divisions. We further show that cell cycle timing of CENP-A assembly is independent of centromeric DNA sequences and instead is mediated by the CENP-A targeting domain. Unexpectedly, this domain also induces stable transmission of centromeric nucleosomes, independent of the CENP-A deposition factor HJURP. This demonstrates that intrinsic properties of the CENP-A protein direct its cell cycle-restricted assembly and induces quantitative mitotic transmission of the CENP-A/H4 nucleosome core, ensuring long-term stability and epigenetic maintenance of centromere position.FCT fellpwships: (SFRH/BD/74284/2010, SFRH/BPD/69115/2010), National Institutes of Health grant: (GM082989), Burroughs Wellcome Fund (Career Award in the Biomedical Sciences), Rita Allen Foundation Scholar Award, Instituto Gulbenkian de Ciência, European Commission FP7 Program, EMBO

Conservation value of the native Hungarian wild grape (Vitis sylvestris Gmel.) evaluated by microsatellite markers

Wild grape (Vitis sylvestris GMEL.) has became a highly threatened species in Europe because of habitat loss, competition with alien grape species and intensive forest exploitation. Twenty-three Vitis spp. samples were analysed at 8 microsatellite loci to estimate the genetic diversity of the natural Vitis sylvestris specimens. In order to analyse the morphological traits and to perform molecular analysis, 11 native individuals were sampled from 6 remnant Hungarian habitats of the wild grape. To compare the genetic relationships among the wild specimens, samples from Turkish habitats, as well as cultivars of Vitis vinifera, Vitis labrusca and Vitis riparia were also included. Genetic diversity was higher within the Hungarian wild grape locations, with a mean of He = 0.74, which was higher that of samples originating from a larger area of Turkey, He = 0.69. Most of the Hungarian samples formed a well-defined, separate branch on the NJ tree. Based on the morphological traits and molecular analysis on the territory of Szentendre Island, formerly considered to be one of the largest native locations of wild grape, interspecific hybrids of Vitis sylvestris and Vitis riparia were identified. It can be concluded from the results that most of the Hungarian habitats studied could be valuable for the conservation. This paper reports on Hungarian Vitis sylvestris habitats, providing the first genetic study on diversity and on the relationship of Vitis sylvestris to other Vitis specimens, wild or cultivated, in the central part of the Carpathian Basin

1991 Archaeological Excavations at the Charles Carroll House in Annapolis, Maryland, 18AP45

This report provides a detailed summary of archaeological excavations that were conducted by Archaeology in Annapolis inside the ground story of the Charles Carroll House in Annapolis (18AP45) during the summer and fall of 1991. This project was initiated by Charles Carroll House of Annapolis, Inc. (CCHA), and was made possible through an agreement between CCHA and Historic Annapolis Foundation. It was designed as an initial phase of a larger project to restore the Carroll House to its late 18th-century appearance, while at the same time adding modern facilities to accomodate receptions, conferences, and other adaptive uses. These excavations were conducted between June and mid October of 1991, prior to interior house restoration, with monitoring of site restoration activities continuing well into 1992. Archaeologists, working with fieldschool students, and volunteers, tested all identified rooms in the house's ground story and then expanded excavations as deemed necessary and as time permitted. In designing the project and in preparing this final report, the staff followed the "Guidelines for Archaeological Investigations in Maryland" (McNarnara 1981). The report includes several levels of summaries (from descriptive summaries of soil levels excavated from the individual units (Appendix A), to interpretive room summaries) in an effort to make the data easily accessible and understandable to archaeologists and others interested in this site

The heart in sporadic inclusion body myositis: a study in 51 patients

The purpose of this study was to explore the prevalence and nature of cardiac abnormalities in sporadic inclusion body myositis (sIBM). Fifty-one sIBM patients were cross-sectionally studied using history-taking, physical examination, measurements of serum creatine kinase activity, the MB fraction (CK-MB), cardiac troponin T (cTnT) and I (cTnI), a 12-lead electrocardiogram (ECG) and 2-dimensional echocardiography. Present cardiac history was abnormal in 12 (24%) out of 51 patients, 12 (24%) patients had abnormalities on ECG, mostly aspecific, and in 12 (24%) patients the echocardiograph showed abnormalities. Elevated CK-MB was present in 42 (82%) patients and 40 (78%) had an elevated cTnT in the absence of acute cardiac pathology. In contrast, in one patient (2%) cTnI was elevated. There was no apparent association between elevated biomarkers, ECG or echocardiographic abnormalities. The prevalence of cardiac abnormalities in sIBM does not seem to be higher than would be expected in these elderly patients. Elevated CK-MB and cTnT levels are common, in contrast to cTnI, but do not reflect cardiac pathology