Quantitative sensory testing of the equine face

Background: Quantitative sensory testing methods are now standard in the evaluation of sensory function in man, while few normal equine values have been reported. Objectives: The aim of this experimental study was (a) to define the tactile sensory, mechanical nociceptive and thermal nociceptive thresholds of the equine face; (b) to assess the effect of age, sex, stimulation site and shaving; (c) to evaluate the reliability of the methods and (d) to provide reference facial quantitative sensory testing values. Study design: Method description. Methods: Thirty-four healthy Warmblood horses were used in the study. Six (tactile sensory threshold) and five (mechanical nociceptive and thermal nociceptive thresholds) areas of the left side of the face with clear anatomical landmarks were evaluated. Ten horses had two (mechanical nociceptive threshold) or three (tactile sensory and thermal nociceptive thresholds) of these areas shaved for another study. A linear Mixed model was used for data analysis. Results: All thresholds increased with age (tactile sensory threshold: by 0.90 g/y (CI = [0.12 g; 0.36 g]) P = .001; mechanical nociceptive threshold: by 0.25 N/y (CI = [0.13-0.36 N]) P = .000; thermal nociceptive threshold: by 0.2°C/y (CI = [0.055-0.361]) P = .008). Sex had no effect on thresholds (tactile sensory threshold: P = .1; mechanical nociceptive threshold: P = .09; thermal nociceptive threshold: P = .2). Stimulation site affected tactile sensory and mechanical nociceptive thresholds (P = .001 and P = .008), but not thermal nociceptive threshold (P = .9). Shaving had no significant effect on any of the thresholds (tactile sensory threshold: P = .06; mechanical nociceptive threshold: P = .08; thermal nociceptive threshold: P = .09). Main limitations: Only the left side was investigated and measurements were obtained on a single occasion. Conclusions: Handheld quantitative sensory testing does not require shaving or clipping to provide reliable measurements. Stimulation over the nostril (tactile sensory threshold), temporomandibular joint (mechanical nociceptive threshold) and supraorbital foramen (thermal nociceptive threshold) resulted in the most consistent thresholds

Four classes of interactions for evolutionary games

The symmetric four-strategy games are decomposed into a linear combination of 16 basis games represented by orthogonal matrices. Among these basis games four classes can be distinguished as it is already found for the three-strategy games. The games with self-dependent (cross-dependent) payoffs are characterized by matrices consisting of uniform rows (columns). Six of 16 basis games describe coordination-type interactions among the strategy pairs and three basis games span the parameter space of the cyclic components that are analogous to the rock-paper-scissors games. In the absence of cyclic components the game is a potential game and the potential matrix is evaluated. The main features of the four classes of games are discussed separately and we illustrate some characteristic strategy distributions on a square lattice in the low noise limit if logit rule controls the strategy evolution. Analysis of the general properties indicates similar types of interactions at larger number of strategies for the symmetric matrix games. © 2015 American Physical Society

Current without bias and diode effect in shuttling transport of nanoshafts

A row of parallely ordered and coupled molecular nanoshafts is shown to develop a shuttling transport of charges at finite temperature. The appearance of a cu rrent without applying an external bias voltage is reported as well as a natura l diode effect allowing unidirectional charge transport along one field directi on while blocking the opposite direction. The zero-bias voltage current appears above a threshold of initial thermal and/or dislocation energy

Thyrotropin-releasing hormone (TRH) promotes wound re-epithelialisation in frog and human skin

There remains a critical need for new therapeutics that promote wound healing in patients suffering from chronic skin wounds. This is, in part, due to a shortage of simple, physiologically and clinically relevant test systems for investigating candidate agents. The skin of amphibians possesses a remarkable regenerative capacity, which remains insufficiently explored for clinical purposes. Combining comparative biology with a translational medicine approach, we report the development and application of a simple ex vivo frog (Xenopus tropicalis) skin organ culture system that permits exploration of the effects of amphibian skin-derived agents on re-epithelialisation in both frog and human skin. Using this amphibian model, we identify thyrotropin-releasing hormone (TRH) as a novel stimulant of epidermal regeneration. Moving to a complementary human ex vivo wounded skin assay, we demonstrate that the effects of TRH are conserved across the amphibian-mammalian divide: TRH stimulates wound closure and formation of neo-epidermis in organ-cultured human skin, accompanied by increased keratinocyte proliferation and wound healing-associated differentiation (cytokeratin 6 expression). Thus, TRH represents a novel, clinically relevant neuroendocrine wound repair promoter that deserves further exploration. These complementary frog and human skin ex vivo assays encourage a comparative biology approach in future wound healing research so as to facilitate the rapid identification and preclinical testing of novel, evolutionarily conserved, and clinically relevant wound healing promoters

Histological assessment of paxgene tissue fixation and stabilization reagents

Within SPIDIA, an EC FP7 project aimed to improve pre analytic procedures, the PAXgene Tissue System (PAXgene), was designed to improve tissue quality for parallel molecular and morphological analysis. Within the SPIDIA project promising results were found in both genomic and proteomic experiments with PAXgene-fixed and paraffin embedded tissue derived biomolecules. But, for this technology to be accepted for use in both clinical and basic research, it is essential that its adequacy for preserving morphology and antigenicity is validated relative to formalin fixation. It is our aim to assess the suitability of PAXgene tissue fixation for (immuno)histological methods. Normal human tissue specimens (n = 70) were collected and divided into equal parts for fixation either with formalin or PAXgene. Sections of the obtained paraffin-embedded tissue were cut and stained. Morphological aspects of PAXgene-fixed tissue were described and also scored relative to formalin-fixed tissue. Performance of PAXgene-fixed tissue in immunohistochemical and in situ hybridization assays was also assessed relative to the corresponding formalin-fixed tissues. Morphology of PAXgene-fixed paraffin embedded tissue was well preserved and deemed adequate for diagnostics in most cases. Some antigens in PAXgene-fixed and paraffin embedded sections were detectable without the need for antigen retrieval, while others were detected using standard, formalin fixation based, immunohistochemistry protocols. Comparable results were obtained with in situ hybridization and histochemical stains. Basically all assessed histological techniques were found to be applicable to PAXgene-fixed and paraffin embedded tissue. In general results obtained with PAXgene-fixed tissue are comparable to those of formalin-fixed tissue. Compromises made in morphology can be called minor compared to the advantages in the molecular pathology possibilities