Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster

Electric shock is a common stimulus for nociception-research and the most widely used reinforcement in aversive associative learning experiments. Yet, nothing is known about the mechanisms it recruits at the periphery. To help fill this gap, we undertook a genome-wide association analysis using 38 inbred Drosophila melanogaster strains, which avoided shock to varying extents. We identified 514 genes whose expression levels and/or sequences covaried with shock avoidance scores. We independently scrutinized 14 of these genes using mutants, validating the effect of 7 of them on shock avoidance. This emphasizes the value of our candidate gene list as a guide for follow-up research. In addition, by integrating our association results with external protein-protein interaction data we obtained a shock avoidance- associated network of 38 genes. Both this network and the original candidate list contained a substantial number of genes that affect mechanosensory bristles, which are hairlike organs distributed across the fly's body. These results may point to a potential role for mechanosensory bristles in shock sensation. Thus, we not only provide a first list of candidate genes for shock avoidance, but also point to an interesting new hypothesis on nociceptive mechanisms

The Dexi-SH* model for a multivariate assessment of agro-ecological sustainability of dairy grazing systems

Dexi-SH* is an ex ante multivariate model for assessing the sustainability of dairy cows grazing systems. This model is composed of three sub-models that evaluate the impact of the systems on: (i) biotic resources; (ii) abiotic resources, and (iii) pollution risks. The structuring of the hierarchical tree was inspired by that of the Masc model. The choice of criteria and their aggregation modalities were discussed within a multi-disciplinary group of scientists. For each cluster, a utility function was established in order to determine weighting and priority functions between criteria. The model can take local and regional conditions and standards into account by adjusting criterion categories to the agroecological context, and the specific views of the decision makers by changing the weighting of criteria

Using indicators to assess the environmental impacts of wine growing activity: The INDIGO (R) method

International audienceEnvironmental assessment methods are needed by agronomists working on the enhancement of cropping systems to meet the demand for more sustainable farming practices. A growing number of operational methods based on a set of indicators have been designed, more for arable crops and livestock than for perennial crops like viticulture. Among them, the INDIGO(R) method, originally developed for arable crops, offers a compromise between feasibility and predictive quality. Here we present a modified and expanded version of INDIGO(R) for viticulture. The development of new indicators specific to viticulture and the adaptation of existing ones followed a five step approach: (i) preliminary definition of the objectives and identification of the end-users, (ii) construction of the indicator, (iii) selection of a reference value, (iv) sensitivity analysis and (v) validation. Stakeholders from professional institutions and winegrower organizations were closely associated with step (i) to define the framework and step (ii) to supply technical databases. We designed INDIGO(R) indicators with all available scientific and expert knowledge which was aggregated into expert systems associating fuzzy subsets or, when possible, quantitative equations. Four indicators; pesticides, nitrogen, energy and soil organic matter, were directly adapted from the initial INDIGO(R) method, whereas soil cover and frost protection management were new indicators. Potentialities of their use are highlighted by examples of implementation on different scales and for various purposes

Environmental assessment of pesticides in vineyards : I-Phy indicator

National audienc

Elaboration d'un indicateur couverture du sol en viticulture

International audienc

Elaboration d'un indicateur couverture du sol en viticulture

International audienc

Adaptation of a pesticide indicator to the watershed scale

International audienc

From field to watershed scale : adaptation of a vineyard pesticide indicator

International audienc

Adaptation of a pesticide indicator to the watershed scale

International audienc