Under-Ice Water Quality Assessment in Perch Lake, Mud Lake, and Kimballs Bay

Previous studies have found lower-than-expected dissolved oxygen (DO) concentrations in a number of bays of the St. Louis River estuary. These hypoxic conditions, which can negatively impact fish and other organisms, are a concern for natural resource managers and anglers. In the current study, water quality was investigated under the ice in March 2020 at Perch Lake, Mud Lake, and Kimballs Bay. DO concentrations varied among sites but were consistent with previous findings, with higher DO near connections to the estuary than farther away, and higher at middle than at lower depths. This research indicates the importance of water exchange in maintaining higher DO concentrations, and the need for future research on mechanisms responsible for low DO in these bays

Substrates of Factor XIII-A: roles in thrombosis and wound healing

FXIII (Factor XIII) is a Ca2+-dependent enzyme which forms covalent ϵ-(γ-glutamyl)lysine cross-links between the γ-carboxy-amine group of a glutamine residue and the ϵ-amino group of a lysine residue. FXIII was originally identified as a protein involved in fibrin clot stabilization; however, additional extracellular and intracellular roles for FXIII have been identified which influence thrombus resolution and tissue repair. The present review discusses the substrates of FXIIIa (activated FXIII) involved in thrombosis and wound healing with a particular focus on: (i) the influence of plasma FXIIIa on the formation of stable fibrin clots able to withstand mechanical and enzymatic breakdown through fibrin–fibrin cross-linking and cross-linking of fibrinolysis inhibitors, in particular α2-antiplasmin; (ii) the role of intracellular FXIIIa in clot retraction through cross-linking of platelet cytoskeleton proteins, including actin, myosin, filamin and vinculin; (iii) the role of intracellular FXIIIa in cross-linking the cytoplasmic tails of monocyte AT1Rs (angiotensin type 1 receptors) and potential effects on the development of atherosclerosis; and (iv) the role of FXIIIa on matrix deposition and tissue repair, including cross-linking of extracellular matrix proteins, such as fibronectin, collagen and von Willebrand factor, and the effects on matrix deposition and cell–matrix interactions. The review highlights the central role of FXIIIa in the regulation of thrombus stability, thrombus regulation, cell–matrix interactions and wound healing, which is supported by observations in FXIII-deficient humans and animals