Heterogeneous responses of dorsal root ganglion neurons in neuropathies induced by peripheral nerve trauma and the antiretroviral drug stavudine

© 2014 The Authors. European Journal of Pain published by John Wiley & Sons Ltd on behalf of European Pain Federation - EFIC®. Funding sources E.K.B. was funded by a BBSRC PhD studentship. A.N., A.S.C.R. and T.P. were funded by a Wellcome Trust Strategic Award (London Pain Consortium; ref. 083259). A.S.C.R. and W.H. were funded by the Innovative Medicines Initiative Joint Undertaking (Europain; grant agreement no. 115007). We thank Pfizer for providing stavudine. Conflicts of interest None declared. Funded by BBSRC PhD studentship Wellcome Trust Strategic Award. Grant Number: 083259 Innovative Medicines Initiative Joint Undertaking. Grant Number: 115007Peer reviewedPublisher PD

Gender and Water Management Practices in Ghana

This article discusses gender and water management practices in the Asante Akim South District in the Ashanti region of Ghana. Using a multi-stage sampling technique, eight communities from four out of six towns operating under the Phase III of the Rural Water Supply Project (RWSP) were selected. The study reveals a difference in the level of participation of men and women in decision-making and planning in RWSP. Fear, criticisms from other women, domestic and economic activities were major challenges to women’s participation in RWSP. The study concludes that, though women showed high participation in the management of RWSP, their participation in decision-making and planning was low. The study demonstrates that since gender plays a vital role in water management, it is crucial that men and women be educated on the importance of women’s active role in decision-making and planning in RWSP, to enhance its effective management. Key words: gender, rural, water supply, water management, participation, communit

A matching scheme for aquaculture; a graphing calculator approach

Graphing Calculator Technology possesses a lot of capabilities in solving a variety of scientific and non-scientific problems. Its programming abilities make it very flexible to use. Unfortunately, it has been underutilised. In this paper, we develop an application with the graphing calculator to handle the processes in a Matching Scheme for Assigning Fish Types to Ponds. The application gives an inclusion matrix which is used as the basis of the matching and finally gives summary of the assignment..Journal of Science and Technology (Ghana) Vol. 27 (2) 2007: pp. 99-10

Age of Transplant and Row Spacing Effects on Growth, Yield and Yield Components of Chilli Pepper (Capsicum annuum L.)

Two field experiments were conducted for two years (2013 and 2014) at the Multipurpose crop nursery of the University of Education, Winneba, Mampong-campus from May to September to evaluate the effect of three transplanting ages (30, 37 and 44 days) and four row spacing (30 x 30 cm, 40 x 30 cm, 50 x 30 cm and 60 x 30cm) on growth, yield and yield components of pepper. The experimental design used was a 3 x 4 factorial in randomized complete block design with three replicates for both experiments. The results showed that 44 aged transplants had the highest yield during the 2013 cropping season, tallest plant in both cropping seasons, highest number of branches and canopy width for the entire 2014 cropping season. The 30 aged transplants differed significantly from the other aged transplants in number of fruits per plant and widest fruit diameter during the 2013 cropping season. The 40 x 30 cm row spacing had the tallest plant and highest number of branches from 8 to 10 weeks after transplanting in both cropping seasons. The 30 x 30 cm row spacing differed significantly from the 50 x 30 cm and 60 x 30 cm row spacing in fruit yield during the 2013 cropping season. The 60 x 30 cm row spacing had the longest fruit length and the heaviest fruit weight per plant during the 2013 cropping season. It is concluded that for high fruit yield, farmers are to transplant pepper seedlings at 44 days using 30 x 30 cm row spacing

Surgical management of constrictive pericarditis

Background: Constrictive pericarditis is a disease characterized by marked thickening and dense scarring of the pericardium with pericardial sac obliteration, or calcification of the pericardium. Without treatment this disease is characterized by high morbidity and mortality.Objective: To review the surgical management of constructivepericarditis and the post operative challenges. Methods: Eleven patients who had pericardiectomy for constructive pericarditis between 2000 and 2005 were studied. Data was obtained from the operating theatre register, histopathological reports and patient’s casenotes. Results: The mean age was 33 years with a range of 14to 53 years. There were seven males (63.6%) and four females (36.4%). Seven (63.6%) out of the eleven patients operated were treated for pulmonary tuberculosis. The cause of pericardial constriction in four patients (36.4%) was undetermined. Follow up period was between 4-59 months. The mean follow up was 17.5 months. Seven patients (63.6%) were off diuretics and had no exercise intolerance. Patients were classified using the New York Heart Association (NYHA) n (NYHA) functional and therapeutic classification in class I-V. Two patients preoperatively in class III are now in class I after surgery on low dose diuretics. One patient who had calcific constrictive pericarditis and came in class III was now in class II with diuretics after 3 years of follow up. There was no postoperative mortality. One patient was lost to follow up. Conclusion: Pericardiectomy is a useful procedure for constrictive pericarditis and was beneficial to all thepatients in this study with an improvement in theirfunctional capacity. Intensive peri-operative monitoringand management reduced morbidity and mortality

Catch rate of juveniles Ethamatosa fimbriata, Sardinella maderensis, and Brachydeuterus auritus fishing in Freetown Peninsular

We investigated the incidences of juvenile fishing according to fishing practices in Freetown peninsular. The industrial trawl fishing sector, despite its technology is not fully selective to avoid bycatch of juvenile fish, and artisanal fishing is so less for economic reasons. Ineffective enforcement of fishing regulations is a major cause to the catch of both targeted and non-targeted juvenile fish. We collected data on the quantity of juvenile fish and the daily duration of fishing trips in four landing sites over a two-week period Bernoulli random variables and properties of uniform distribution were used to analyze the data. Catch rates of juveniles Ethamatosa fimbriata, Sardinella maderensis, and Brachydeuterus auritus around the Freetown peninsular were 170.8, 212.4 and 194.60 kg per hour per day, respectively. This rate revealed that the current trend of juvenile fish landings is a threat to the sustainability of fishery capital, revenue, and biodiversity.Key words: High juvenile, landing, national problem, Sierra Leone, by-catch juvenile

Dynamics of genotype-specific HPV clearance and reinfection in rural Ghana may compromise HPV screening approaches

Persistent Human Papillomavirus (HPV) infection is a prerequisite for cervical cancer development. Few studies investigated clearance of high-risk HPV in low-and-middle-income countries. Our study investigated HPV clearance and persistence over four years in women from North Tongu District, Ghana. In 2010/2011, cervical swabs of 500 patients were collected and HPV genotyped (nested multiplex PCR) in Accra, Ghana. In 2014, 104 women who previously tested positive for high-risk HPV and remained untreated were re-tested for HPV. Cytobrush samples were genotyped (GP5+/6+ PCR & Luminex-MPG readout) in Berlin, Germany. Positively tested patients underwent colposcopy and treatment if indicated. Of 104 women, who tested high-risk HPV+ in 2010/2011, seven (6,7%; 95%CI: 2.7-13.4%) had ≥1 persistent high-risk-infection after ~4 years (mean age 39 years). Ninety-seven (93,3%; 95%CI: 86.6-97.3%) had cleared the original infection, while 22 (21.2%; 95%CI: 13.8-30.3%) had acquired new high-risk infections with other genotypes. Persistent types found were HPV 16, 18, 35, 39, 51, 52, 58, and 68. Among those patients, one case of CIN2 (HPV 68) and one micro-invasive cervical cancer (HPV 16) were detected. This longitudinal observational data suggest that single HPV screening rounds may lead to over-referral. Including type-specific HPV re-testing or additional triage methods could help reduce follow-up rates

Pattern and Outcome of Chest Injuries at Bugando Medical Centre in Northwestern Tanzania.

Chest injuries constitute a continuing challenge to the trauma or general surgeon practicing in developing countries. This study was conducted to outline the etiological spectrum, injury patterns and short term outcome of these injuries in our setting. This was a prospective study involving chest injury patients admitted to Bugando Medical Centre over a six-month period from November 2009 to April 2010 inclusive. A total of 150 chest injury patients were studied. Males outnumbered females by a ratio of 3.8:1. Their ages ranged from 1 to 80 years (mean = 32.17 years). The majority of patients (72.7%) sustained blunt injuries. Road traffic crush was the most common cause of injuries affecting 50.7% of patients. Chest wall wounds, hemothorax and rib fractures were the most common type of injuries accounting for 30.0%, 21.3% and 20.7% respectively. Associated injuries were noted in 56.0% of patients and head/neck (33.3%) and musculoskeletal regions (26.7%) were commonly affected. The majority of patients (55.3%) were treated successfully with non-operative approach. Underwater seal drainage was performed in 39 patients (19.3%). One patient (0.7%) underwent thoracotomy due to hemopericardium. Thirty nine patients (26.0%) had complications of which wound sepsis (14.7%) and complications of long bone fractures (12.0%) were the most common complications. The mean LOS was 13.17 days and mortality rate was 3.3%. Using multivariate logistic regression analysis, associated injuries, the type of injury, trauma scores (ISS, RTS and PTS) were found to be significant predictors of the LOS (P < 0.001), whereas mortality was significantly associated with pre-morbid illness, associated injuries, trauma scores (ISS, RTS and PTS), the need for ICU admission and the presence of complications (P < 0.001). Chest injuries resulting from RTCs remain a major public health problem in this part of Tanzania. Urgent preventive measures targeting at reducing the occurrence of RTCs is necessary to reduce the incidence of chest injuries in this region

Quality of glycemic control in type 2 diabetes mellitus (T2DM) and its association with markers of coagulation and inhibitors of fibrinolysis: A case–control study in the upper west region, Ghana

Background and Aims: Type 2 diabetes mellitus (T2DM) individuals are at a higher risk of developing diabetes complications, with approximately 80% complication-related mortality. The increased morbidity and mortality among T2DM patients are partly due to dysregulated hemostasis. This study determined the quality of glycemic control in T2DM and its association with markers of coagulation and inhibitors of fibrinolysis. Methods: This case–control study recruited 90 participants involving: 30 T2DM patients with good glycemic control, 30 with poor glycemic control, and 30 nondiabetic subjects as controls at a Municipal Hospital in Ghana. Fasting blood glucose, glycated hemoglobin, activated partial thromboplastin time (APTT), prothrombin time (PT), calculated international normalized ratio (INR), and full blood count (FBC) were determined for each respondent. Plasma levels of plasminogen activator inhibitor-1 (PAI-1) and thrombin activatable fibrinolysis inhibitor (TAFI) were determined using the solid-phase sandwich enzyme-linked immunosorbent assay method. Data were analyzed using R language software. Results: Plasma PAI-1 antigen levels were significantly higher in the participants with poor glycemic control as compared to participants with good glycemic control (p \u3c 0.0001). There was no significant difference in plasma TAFI levels between the participants with poor glycemic control as compared to participants with good glycemic control (p = 0.900). T2DM patients had significantly shorter APTT, PT, and INR than controls (p \u3c 0.05). At a cut-off of ≥ 161.70 pg/μL, PAI was independently associated with increasing odds (adjusted odds ratio = 13.71, 95% confidence interval: 3.67–51.26, p \u3c 0.0001) of poor glycemic control and showed the best diagnostic accuracy for poor glycemic control (area under the curve = 0.85, p \u3c 0.0001). Conclusion: PAI-1 levels were significantly increased in T2DM with poor glycemic control and emerged as the best predictor for poor glycemic control. Good glycemic management to control the plasma levels of PAI-1 is required to prevent hypercoagulability and thrombotic disorders