Mutations in human dynamin block an intermediate stage in coated vesicle formation

The role of human dynamin in receptor-mediated endocytosis was investigated by transient expression of GTP-binding domain mutants in mammalian cells. Using assays which detect intermediates in coated vesicle formation, the dynamin mutants were found to block endocytosis at a stage after the initiation of coat assembly and preceding the sequestration of ligands into deeply invaginated coated pits. Membrane transport from the ER to the Golgi complex was unaffected indicating that dynamin mutants specifically block early events in endocytosis. These results demonstrate that mutations in the GTP-binding domain of dynamin block Tfn-endocytosis in mammalian cells and suggest that a functional dynamin GTPase is required for receptor-mediated endocytosis via clathrin-coated pits

Dust Emission from Herbig Ae/Be stars - Evidence for Disks and Envelopes

IR and mm-wave emission from Herbig Ae/Be stars has produced conflicting conclusions regarding the dust geometry in these objects. We show that the compact dimensions of the mm-wave emitting regions are a decisive indication for disks. But a disk cannot explain the spectral energy distribution (SED) unless it is embedded in an extended envelope that (1) dominates the IR emission and (2) provides additional disk heating on top of the direct stellar radiation. Detailed radiative transfer calculations based on the simplest model for envelope-embedded disks successfully fit the data from UV to mm wavelengths and show that the disks have central holes. This model also resolves naturally some puzzling results of IR imaging.Comment: 9 pages, 2 figures. accepted to ApJ

The Brown Dwarf Kinematics Project (BDKP). III. Parallaxes for 70 Ultracool Dwarfs

We report parallax measurements for 70 ultracool dwarfs (UCDs). Using both literature values and our sample, we report new polynomial relations between spectral type and M$_{JHK}$. Including resolved L/T transition binaries in the relations, we find no reason to differentiate between a "bright" (unresolved binary) and "faint" (single source) sample across the L/T boundary. Isolating early T dwarfs, we find that the brightening of T0-T4 sources is prominent in M$_{J}$ where there is a [1.2 - 1.4] magnitude difference. A similar yet dampened brightening of [0.3 - 0.5] magnitude happens at M$_{H}$ and a plateau or dimming of [-0.2 - -0.3] magnitude is seen in M$_{K}$. Comparing with evolutionary models that vary gravity, metallicity, and cloud thickness we find that a near constant temperature of 1200 $\pm$100 K along a narrow spectral subtype of T0-T4 is required to account for the brightening and color magnitude diagram of the L-dwarf/T-dwarf transition. Furthermore, there is a significant population of both L and T dwarfs which are red or potentially "ultra-cloudy" compared to the models, many of which are known to be young indicating a correlation between enhanced photospheric dust and youth. For the low surface-gravity or young companion L dwarfs we find that 8 out of 10 are at least [0.2-1.0] magnitude underluminous in M$_{JH}$ and/or M$_{K}$ compared to equivalent spectral type objects. We speculate that this is a consequence of increased dust opacity and conclude that low-surface gravity L dwarfs require a completely new spectral-type/absolute magnitude polynomial for analysis.Comment: 65 pages, Accepted for publication to Ap

High-resolution imaging at the SOAR telescope

Bright single and binary stars were observed at the 4.1-m telescope with a fast electron-multiplication camera in the regime of partial turbulence correction by the visible-light adaptive optics system. We compare the angular resolution achieved by simple averaging of AO-corrected images (long-exposure), selection and re-centering (shift-and-add or "lucky" imaging) and speckle interferometry. The effect of partial AO correction, vibrations, and image post-processing on the attained resolution is shown. Potential usefulness of these techniques is evaluated for reaching the diffraction limit in ground-based optical imaging. Measurements of 75 binary stars obtained during these tests are given and objects of special interest are discussed. We report tentative resolution of the astrometric companion to Zeta Aqr B. A concept of advanced high-resolution camera is outlined.Comment: Accepted for publication in PASP. 14 pages, 9 figures, 2 tabl

Color Transformations for the 2MASS Second Incremental Data Release

Transformation equations are presented to convert colors and magnitudes measured in the AAO, ARNICA, CIT, DENIS, ESO, LCO (Persson standards), MSSSO, SAAO, and UKIRT photometric systems to the photometric system inherent to the 2MASS Second Incremental Data Release. The transformations have been derived by comparing 2MASS photometry with published magnitudes and colors for stars observed in these systems. Transformation equations have also been derived indirectly for the Bessell & Brett (1988) and Koornneef (1983) homogenized photometric systems.Comment: To appear in AJ, May 200

L- and M-band imaging observations of the Galactic Center region

We present near-infrared H-, K-, L- and M-band photometry of the Galactic Center from images obtained at the ESO VLT in May and August 2002, using the NAOS/CONICA (H and K) and the ISAAC (L and M) instruments. The large field of view (70" x 70") of the ISAAC instrument and the large number of sources identified (L-M data for 541 sources) allows us to investigate colors, infrared excesses and extended dust emission. Our new L-band magnitude calibration reveals an offset to the traditionally used calibrations, which we attribute to the use of the variable star IRS7 as a flux calibrator. Together with new results on the extinction towards the Galactic Center (Scoville et al. 2003; Raab 2000), our magnitude calibration results in stellar color properties expected from standard stars and removes any necessity to modify the K-band extinction. The large number of sources for which we have obtained L-M colors allows us to measure the M-band extinction to A_M=(0.056+-0.006)A_V (approximately =A_L), a considerably higher value than what has so far been assumed. L-M color data has not been investigated previously, due to lack of useful M-band data. We find that this color is a useful diagnostic tool for the preliminary identification of stellar types, since hot and cool stars show a fairly clear L-M color separation. This is especially important if visual colors are not available, as in the Galactic Center. For one of the most prominent dust embedded sources, IRS3, we find extended L- and M-band continuum emission with a characteristic bow-shock shape. An explanation for this appearance is that IRS3 consists of a massive, hot, young mass-losing star surrounded by an optically thick, extended dust shell, which is pushed northwest by wind from the direction of the IRS16 cluster and SgrA*.Comment: 24 pages, 7 figures, 2 tables, accepted for publication in Astronomy & Astrophysic

The Vela Pulsar in the Near-Infrared

We report on the first detection of the Vela pulsar in the near-infrared with the VLT/ISAAC in the Js and H bands. The pulsar magnitudes are Js=22.71 +/- 0.10 and H=22.04 +/- 0.16. We compare our results with the available multiwavelength data and show that the dereddened phase-averaged optical spectrum of the pulsar can be fitted with a power law F_nu propto nu^(-alpha_nu) with alpha_nu = 0.12 +/- 0.05, assuming the color excess E(B-V)=0.055 +/-0.005 based on recent spectral fits of the emission of the Vela pulsar and its supernova remnant in X-rays. The negative slope of the pulsar spectrum is different from the positive slope observed over a wide optical range in the young Crab pulsar spectrum. The near-infrared part of the Vela spectrum appears to have the same slope as the phase-averaged spectrum in the high energy X-ray tail, obtained in the 2-10 keV range with the RXTE. Both of these spectra can be fitted with a single power law suggesting their common origin. Because the phase-averaged RXTE spectrum in this range is dominated by the second X-ray peak of the pulsar light curve, coinciding with the second main peak of its optical pulse profile, we suggest that this optical peak can be redder than the first one. We also detect two faint extended structures in the 1.5''-3.1'' vicinity of the pulsar, projected on and aligned with the south-east jet and the inner arc of the pulsar wind nebula, detected in X-rays with Chandra. We discuss their possible association with the nebula.Comment: 12 pages, 8 figures, accepted for publication in A&A, the associated near-infrared images in the fits format are available at http://www.ioffe.ru/astro/NSG/obs/vela-ir

A Model for the Voltage Steps in the Breakdown of the Integer Quantum Hall Effect

In samples used to maintain the US resistance standard the breakdown of the dissipationless integer quantum Hall effect occurs as a series of dissipative voltage steps. A mechanism for this type of breakdown is proposed, based on the generation of magneto-excitons when the quantum Hall fluid flows past an ionised impurity above a critical velocity. The calculated generation rate gives a voltage step height in good agreement with measurements on both electron and hole gases. We also compare this model to a hydrodynamic description of breakdown.Comment: 4 pages including 3 figure