Preliminary evidence for the role played by south westerly wind strength on the marine diatom content of an Antarctic Peninsula ice core (1980–2010)

Winds in the Southern Ocean drive exchanges of heat and carbon dioxide between the ocean and atmosphere. Wind dynamics also explain the dominant patterns of both basal and surface melting of glaciers and ice shelves in the Amundsen and Bellingshausen Seas. Long records of past wind strength and atmospheric circulation are needed to assess the significance of these recent changes. Here we present evidence for a novel proxy of past south westerly wind (SWW) strength over the Amundsen and Bellingshausen Seas, based on diatoms preserved in an Antarctic Peninsula ice core. Ecological affinities of the identified diatom taxa indicate an almost exclusively marine assemblage, dominated by open ocean taxa from the Northern Antarctic Zone (NAZ). Backtrajectory analysis shows the routes of air masses reaching the ice core site and reveals that many trajectories involve contact with surface waters in the NAZ of the Amundsen and Bellingshausen Seas. Correlation analyses between ice core diatom abundance and various wind vectors yield positive and robust coefficients for the 1980–2010 period, with average annual SWW speeds exhibiting the strongest match. Collectively, the data presented here provide new evidence that diatoms preserved in an Antarctic Peninsula ice core offer genuine potential as a new proxy for SWW strength

Short chain diamines are the physiological substrates of PACE family efflux pumps

Acinetobacter baumannii has rapidly emerged as a major cause of gram-negative hospital infections worldwide. A. baumannii encodes for the transport protein AceI, which confers resistance to chlorhexidine, a widely used antiseptic. AceI is also the prototype for the recently discovered proteobacterial antimicrobial compound efflux (PACE) family of transport proteins that confer resistance to a range of antibiotics and antiseptics in many gram-negative bacteria, including pathogens. The gene encoding AceI is conserved in the core genome of A. baumannii, suggesting that it has an important primordial function. This is incongruous with the sole characterized substrate of AceI, chlorhexidine, an entirely synthetic biocide produced only during the last century. Here we investigated a potential primordial function of AceI and other members of the PACE family in the transport of naturally occurring polyamines. Polyamines are abundant in living cells, where they have physiologically important functions and play multifaceted roles in bacterial infection. Gene expression studies revealed that the aceI gene is induced in A. baumannii by the short-chain diamines cadaverine and putrescine. Membrane transport experiments conducted in whole cells of A. baumannii and Escherichia coli and also in proteoliposomes showed that AceI mediates the efflux of these short-chain diamines when energized by an electrochemical gradient. Assays conducted using 8 additional diverse PACE family proteins identified 3 that also catalyze cadaverine transport. Taken together, these results demonstrate that short-chain diamines are common substrates for the PACE family of transport proteins, adding to their broad significance as a novel family of efflux pumps

Photochemical fingerprinting Is a sensitive probe for the detection of synthetic cannabinoid receptor agonists; toward robust point-of-care detection

With synthetic cannabinoid receptor agonist (SCRA) use still prevalent across Europe and structurally advanced generations emerging, it is imperative that drug detection methods advance in parallel. SCRAs are a chemically diverse and evolving group, which makes rapid detection challenging. We have previously shown that fluorescence spectral fingerprinting (FSF) has the potential to provide rapid assessment of SCRA presence directly from street material with minimal processing and in saliva. Enhancing the sensitivity and discriminatory ability of this approach has high potential to accelerate the delivery of a point-of-care technology that can be used confidently by a range of stakeholders, from medical to prison staff. We demonstrate that a range of structurally distinct SCRAs are photochemically active and give rise to distinct FSFs after irradiation. To explore this in detail, we have synthesized a model series of compounds which mimic specific structural features of AM-694. Our data show that FSFs are sensitive to chemically conservative changes, with evidence that this relates to shifts in the electronic structure and cross-conjugation. Crucially, we find that the photochemical degradation rate is sensitive to individual structures and gives rise to a specific major product, the mechanism and identification of which we elucidate through density-functional theory (DFT) and time-dependent DFT. We test the potential of our hybrid “photochemical fingerprinting” approach to discriminate SCRAs by demonstrating SCRA detection from a simulated smoking apparatus in saliva. Our study shows the potential of tracking photochemical reactivity via FSFs for enhanced discrimination of SCRAs, with successful integration into a portable device

In from the cold: an overview of the British Antarctic Survey fossil collections

The British Antarctic Survey is the custodian of more than 57,000 Antarctic fossils, of which some 2,500 are Type & Figured specimens. In addition to these, the geological collections include over 100,000 rock and mineral specimens representing all the main lithologies. Gathered over the last 60 years, the collections form an invaluable taxonomic and lithographic resource for the study of biodiversity, environmental change and planetary evolution. Methods of analysis are constantly being refined and up-dated so the collection needs to be effectively managed, stored and recorded if it is to continue to function as an investigative resource. Good curation is especially important given the high cost, in both time and money, of collecting specimens from such a remote and hostile environment. For the most part, the material is well documented but more curatorial time needs to be spent on the specimens if they are not to deteriorate through contamination or neglect

Practical Synthesis of Polyamine Succinamides and Branched Polyamines

Antibiotic resistance is now a growing threat to human health, further exacerbated by the lack of new antibiotics. We describe the practical synthesis of a series of substituted polyamine succinamides and branched polyamines that are potential new antibiotics against both Gram-positive and Gram-negative bacteria, including MRSA and Pseudomonas aeruginosa. They are prepared via 1,4-Michael addition of acrylonitrile and then hydrogenation of the nitrile functional groups to primary amines. They are built upon the framework of the naturally occurring polyamines thermine (3.3.3, norspermine) and spermine (3.4.3), homo- and heterodimeric polyamine succinic amides. Linking two of the same or different polyamines together via amide bonds can be achieved by introducing a carboxylic acid group on the first polyamine, then coupling that released carboxylic acid to a free primary amine in the second polyamine. If the addition of positive charges on the amino groups along the polyamine chains are a key factor in their antimicrobial activity against Gram-negative bacteria, then increasing them will increase the antimicrobial activity. Synthesising polyamine amide dimers will increase the total net positive charge compared to their monomers. The design and practical synthesis of such homo- and hetero-dimers of linear polyamines, spermine and norspermine, are reported. Several of these compounds do not display significant antibacterial activity against Gram-positive or Gram-negative bacteria, including MRSA and Pseudomonas aeruginosa. However, the most charged analogue, a branched polyamine carrying eight positive charges at physiological pH, displays antibiofilm activity with a 50 % reduction in PAO1 at 16-32 μg mL-1 .</p