88 research outputs found
Digitalisierung: Perspektiven für Arbeitsmodelle der Zukunft in Wirtschaft und Verwaltung
Die Digitalisierung ist derzeit einer der wichtigsten Wandlungstreiber in Wirtschaft und Gesellschaft. Die aus den neuen technischen Möglichkeiten resultierenden Veränderungen wirken sich auch auf das Verhalten von Kunden und Mitarbeitenden aus. In Bezug auf die Zusammenarbeit in Unternehmen und Organisationen sind bestehende Arbeitsmodelle für administrative Arbeit anzupassen und neue Formen digitaler Kollaboration einzuführen. Hiermit verbunden sind Änderungen der Arbeitszeitmodelle, der Organisation der Mitarbeitenden, der Art der Zusammenarbeit und Führung sowie der Gestaltung von Büroräumlichkeiten. In einem vergleichenden Case-Study-Ansatz von zwei Schweizer Großunternehmen und einer großen Verwaltungseinheit des Bundes werden die existierenden Arbeitsmodelle für Büroarbeit analysiert und auf Entwicklungsperspektiven hin untersucht. Ein derzeit vorhandener Mix aus traditionellen und agilen Arbeitsmodellen wird sich in Zukunft im digitalen Kontext voraussichtlich unternehmensspezifisch stärker in Richtung einer höheren Agilität weiterentwickeln
Histone H2A and H2B Are Monoubiquitinated at AID-Targeted Loci
Background: Somatic hypermutation introduces base substitutions into the rearranged and expressed immunoglobulin (Ig) variable regions to promote immunity. This pathway requires and is initiated by the Activation Induced Deaminase (AID) protein, which deaminates cytidine to produce uracils and UG mismatches at the Ig genes. Subsequent processing of uracil by mismatch repair and base excision repair factors contributes to mutagenesis. While selective for certain genomic targets, the chromatin modifications which distinguish hypermutating from non-hypermutating loci are not defined. Methodology/Principal Findings: Here, we show that AID-targeted loci in mammalian B cells contain ubiquitinated chromatin. Chromatin immunoprecipitation (ChIP) analysis of a constitutively hypermutating Burkitt\u27s B cell line, Ramos, revealed the presence of monoubiquitinated forms of both histone H2A and H2B at two AID-associated loci, but not at control loci which are expressed but not hypermutated. Similar analysis using LPS activated primary murine splenocytes showed enrichment of the expressed V(H) and S gamma 3 switch regions upon ChIP with antibody specific to AID and to monoubiquitinated H2A and H2B. In the mechanism of mammalian hypermutation, AID may interact with ubiquitinated chromatin because confocal immunofluorescence microscopy visualized AID colocalized with monoubiquitinated H2B within discrete nuclear foci. Conclusions/Significance: Our results indicate that monoubiquitinated histones accompany active somatic hypermutation, revealing part of the histone code marking AID-targeted loci. This expands the current view of the chromatin state during hypermutation by identifying a specific nucleosome architecture associated with somatic hypermutation
Correlation between radiographic signs of third molar proximity with inferior alveolar nerve and postoperative occurrence of neurosensory disorders: A prospective, double-blind study
Prey consumption of Orius similis Zheng (Het., Anthocoridae) with different aphid species as prey
The laboratory experiments showed that the predatory bug
Orius similis
Zheng, originally from China, is able to prey on different aphid species. It consumed significantly higher
Aphis gossypii
Glover and
Aphis pomi
(De Geer) than other aphid species during its entire nymphal development and adult stage as females (mated and unmated) and males. There were no significant differences in the mean daily and total prey consumption of females and males during the nymphal development. The mean daily prey consumption of adult
O. similis
was significantly higher by mated females than unmated females and males. No significant differences in the mean daily prey consumption of unmated females and males were detected. When
A. gossypii
and
A. pomi
were offered, the mean total prey consumption was significantly higher by females (mated and unmated) than by males. It was significantly higher by mated females than unmated females and males with
Acyrthosiphon pisum
(Harris) and
Myzus persicae
(Sulzer) as prey.
O. similis
adapted smoothly to fluctuating prey availability that increasing or decreasing prey consumption of the predator is depended on prey population density. The results showed that
O. similis
seems to be a promising predator for biological control against different aphid species
Effect of temperature and light intensity on early infection behaviour of a Western Australian isolate of Plasmopara viticola
Epidemiology of grapevine anthracnose and downy mildew in an Auckland, New Zealand vineyard
Structure of molecular tweezer complexes in the solid state: NMR experiments, X-ray investigations, and quantum chemical calculations
The structure of supramolecular complexes formed by a naphthalene-spaced tweezer molecule as host and 1,4-dicyanobenzene (DCNB), 1,2,4,5-tetracyanobenzene (TCNB), and 7,7,8,8-tetracyano-p-quinodimethane (TCNQ) as aromatic, electron-deficient guests is investigated by solid-state NMR and X-ray diffraction measurements. Quantum chemical calculations using linear scaling methods are applied to predict and to assign the H-1 NMR chemical shifts of the complexes. By combining experiment and theory, insights into intra- and intermolecular effects influencing the proton chemical shifts of the host-guest system are provided in the solid state
Warp-Knitted Spacer Fabrics : A Versatile Platform to Generate Fiber-Reinforced Hydrogels for 3D Tissue Engineering
Mesenchymal stem cells (MSCs) possess huge potential for regenerative medicine. For tissue engineering approaches, scaffolds and hydrogels are routinely used as extracellular matrix (ECM) carriers. The present study investigated the feasibility of using textile-reinforced hydrogels with adjustable porosity and elasticity as a versatile platform for soft tissue engineering. A warp-knitted poly (ethylene terephthalate) (PET) scaffold was developed and characterized with respect to morphology, porosity, and mechanics. The textile carrier was infiltrated with hydrogels and cells resulting in a fiber-reinforced matrix with adjustable biological as well as mechanical cues. Finally, the potential of this platform technology for regenerative medicine was tested on the example of fat tissue engineering. MSCs were seeded on the construct and exposed to adipogenic differentiation medium. Cell invasion was detected by two-photon microscopy, proliferation was measured by the PrestoBlue assay. Successful adipogenesis was demonstrated using Oil Red O staining as well as measurement of secreted adipokines. In conclusion, the given microenvironment featured optimal mechanical as well as biological properties for proliferation and differentiation of MSCs. Besides fat tissue, the textile-reinforced hydrogel system with adjustable mechanics could be a promising platform for future fabrication of versatile soft tissues, such as cartilage, tendon, or muscle
- …