Properties and expression of Na+/K+-ATPase α-subunit isoforms in the brain of the swamp eel, Monopterus albus, which has unusually high brain ammonia tolerance

10.1371/journal.pone.0084298PLoS ONE812-POLN

The Influence of Typhoon “MITAG” on Waves and Currents in Zhoushan Sea Area, China

Typhoon “MITAG” was generated at the end of September 2019 and landed briefly in Zhoushan on October 1. Based on reanalysis data provided by ERA5 and NCEP, this paper analyzes the characteristics of wave and current during “MITAG”. The variation rule of waves and currents in different periods during the influence of “MITAG” was found. The results are as follows: The variation of significant wave height and mean wave period is related to its waveform. The single waveform has a long wave period and the correlation between wave height and wave period reaches 0.87 during the wind wave period. The wave period of the mixed waveform is shorter. The Ekman pumping of the ocean by “MITAG” is concentrated on the right side of the typhoon path when it is away from land; however, Ekman pumping is on the land side when the typhoon is close to the land. The sea surface height of the coastal sea area changes regularly with the distance of “MITAG”. The area which has a strong current is consistent with higher wave height

Effects of the decomposing liquid of Cladophora oligoclona on Hydrilla verticillata turion germination and seedling growth

Excessive proliferation of filamentous green algae (FGA) has been considered an important factor resulting in the poor growth or even decline of submerged macrophytes. However, there is a lack of detailed information regarding the effect of decaying FGA on submerged macrophytes. This study aimed to investigate whether the decomposing liquid from Cladophora oligoclona negatively affects Hydrilla verticillata turion germination and seedling growth. The results showed that the highest concentrations of decomposing liquid treatments inhibited the turion germination rate, which was the lowest than other treatments, at only 84%. The chlorophyll a fluorescence (JIP test) and physiological indicators (chlorophyll a content, soluble sugars, Ca2+/Mg2+-ATPase and PAL activity) were also measured. The chlorophyll a content in the highest concentration (40% of original decomposing liquid) treatment group decreased by 43.53% than that of the control; however, soluble sugars, Ca2+/Mg2+-ATPase, and PAL activity increased by 172.46%, 271.19%, and 26.43% respectively. The overall results indicated that FGA decay has a considerable effect on submerged macrophyte turion germination and seedling growth, which could inhibit their expansion and reproduction. This study emphasized the need to focus on effects of FGA decomposition on the early growth stages of submerged macrophytes and offered technological guidance for submerged vegetation restoration in lakes and shallow waters

Annual changes in periphyton communities and their diatom indicator species, in the littoral zone of a subtropical urban lake restored by submerged plants

Biological indicators of assessing surface waters depend on regionally based indices and sensitivity to environmental conditions. Periphytic algae often used as a potential biological assessment method for its environmental sensitivity characteristics. Here we studied the characteristics of the periphytic algae community and diatom species in the subtropical urban landscape lake known as West Lake, Hangzhou, China, which is in the process of ecological restoration. The changes in species composition, biomass and the community diversity of periphytic algae were determined. 71 taxa of 54 genera of periphytic algae were observed. The Bacillariophyta, Chlorophyta and Cyanophyta dominated in the community. The Bacillariophytes exhibited the highest species richness, whereas the Cyanophytes showed the highest cell density. Principal component analysis environmental variables showed that the TN was main nutrient factor to periphytic algae in the West Lake. In terms of the relatively high TN contents characteristics in subtropical urban landscape lake West Lake, the ecological optimum values and tolerance values of TN about diatom were calculated by weighted regression method. Synedra capitata, Achnanthes minutissima, Gomphonema acuminatum, Navicula graciloides were extracted as indicator species for environmental gradients of TN in the West Lake. Those findings provide a theoretical basis for the use of diatoms biological index to improve the effectiveness of monitoring and evaluating the nutritional level of TN in urban landscape lakes system. It would be significant to identify the nutritional status of the subtropical shallow water bodies and advise related management

Identification of novel Z/W chromosome-specific markers from the giant freshwater prawn Macrobrachium rosenbergii

The giant freshwater prawn (Macrobrachium rosenbergii) is a commercially cultured species in China. A sex chromosome-specific marker is crucial for sex identification and distinguishing sex chromosomes. Here, based on the results of reduced-representation genome sequencing and genome survey of M. rosenbergii, we identified steady sex-linked markers. Further nucleotide alignment analysis revealed 83.42% nucleotide similarity between the amplified W- and Z-chromosome fragments. qPCR confirmed that Z chromosome-specific fragments were present in males approximately twice as often compared to females. Similarly, the W chromosome-specific fragments were present in super females approximately twice as often compared to females. Our findings elucidate the ZW/ZZ sex determination type and contribute to exploring sex-related or sex-determining genes present on the sex chromosomes of M. rosenbergii

The Response of Regeneration Ability of <i>Myriophyllum spicatum</i> Apical Fragments to Decaying <i>Cladophora oligoclona</i>

During the restoration of eutrophic shallow lakes, submerged macrophyte recovery is often accompanied by an excessive proliferation of filamentous green algae (FGA). This can lead to the recession or even disappearance of the submerged macrophytes in these lakes. However, the use of plant fragments in reproduction and dispersion is an important life history strategy for submerged macrophytes. In this work, it studied apical fragment propagation in Myriophyllum spicatum and its physiological and biochemical responses to the decomposing liquid of Cladophora oligoclona. Myriophyllum spicatum apical fragments showed no new roots or buds when treated with 0.4 times the original decomposing liquid, and their PSII functional parameters were lower than those of the control. In contrast, the relevant sugar content accumulated to 115.26%, and the activities of an energy enzyme (Ca2+/Mg2+-ATPase) and a secondary metabolic-related enzyme (PAL) increased by 490.63% and 28.13%, showing an elevated defense response. These results indicated that the early regeneration of M. spicatum could be hindered by environmental stress, and that this may further affect the reproduction and colonization of these submerged macrophytes

Synthesis and Cytotoxic Activity Evaluation of Novel Arylpiperazine Derivatives on Human Prostate Cancer Cell Lines

A series of novel arylpiperazine derivatives was synthesized. The in vitro cytotoxic activities of all synthesized compounds against three human prostate cancer cell lines (PC-3, LNCaP, and DU145) were evaluated by a CCK-8 assay. Compounds 9 and 15 exhibited strong cytotoxic activities against LNCaP cells (IC50 &lt; 5 μM), and compound 8 (IC50 = 8.25 μM) possessed the most potent activity against DU145 cells. However, these compounds also exhibited cytotoxicity towards human epithelial prostate normal cells RWPE-1. The structure–activity relationship (SAR) of these arylpiperazine derivatives was also discussed based on the obtained experimental data