Leydig cells express neural cell adhesion molecules in vivo and in vitro

The neural cell adhesion molecule (NCAM) polypeptides are expressed by numerous tissues during embryonic development, where they are involved in cell-cell interactions. In the adult, NCAM expression is confined to a few cell types, including neurons and peptide-hormone-producing cells. Here we demonstrate that the Leydig cells of the adult rat, mouse, and hamster testes express NCAM as well. Western blotting showed that an NCAM of approximately 120 kDa was present in the adult testes of all three species investigated. This form was also found in freshly isolated mouse Leydig cells and in Leydig cells after 2 days in culture. After 4 days in culture, mouse Leydig cells expressed additional NCAM isoforms of approximately 140 and 180 kDa, indicating changes in alternative splicing of NCAM primary transcripts. Also, NCAM mRNA of all isoforms, as detected by S1-nuclease protection assays, increased with time in culture. The expression of the cell adhesion molecule NCAM by adult Leydig cells may explain the aggregation of Leydig cells in clusters in rodent testes, which could be a prerequisite for functional coordination of groups of Leydig cells. Furthermore, the presence of this neural and endocrine marker may indicate a closer relationship between Leydig cells and neural and peptide-hormone-producing cells than is considered to exist at the present time

Nuclear Magnetic Resonance and Hyperfine Structure

Contains research objectives and reports on three research projects

Communicative competence and psychological aspects when interacting with client in tourism

The article deals with the role of psychological preparation of specialists in service and tourism to building relationships with clients of travel agencies. Attention is paid to what is important in psychological preparation of the expert assigned to the speech skill, because the possession of speech skills influences the formation of the motives and interests of the client, speed, completeness and the strength of assimilation of advertising materials, economy, recall; accuracy, consistency and brightness of managerial playback of the advertised materia

Automated operation of a home made torque magnetometer using LabVIEW

In order to simplify and optimize the operation of our home made torque magnetometer we created a new software system. The architecture is based on parallel, independently running instrument handlers communicating with a main control program. All programs are designed as command driven state machines which greatly simplifies their maintenance and expansion. Moreover, as the main program may receive commands not only from the user interface, but also from other parallel running programs, an easy way of automation is achieved. A program working through a text file containing a sequence of commands and sending them to the main program suffices to automatically have the system conduct a complex set of measurements. In this paper we describe the system's architecture and its implementation in LabVIEW.Comment: 6 pages, 7 figures, submitted to Rev. Sci. Inst

Coherent Ro-vibrational Revivals in a Thermal Molecular Ensemble

We report an experimental and theoretical study of the evolution of vibrational coherence in a thermal ensemble of nitrogen molecules. Rotational dephasing and rephasing of the vibrational coherence is detected by coherent anti-Stokes Raman scattering. The existence of ro-vibrational coupling and the discrete energy spectrum of the rotational bath lead to a whole new class of full and fractional ro-vibrational revivals. Following the rich ro-vibrational dynamics on a nanosecond time scale with sub-picosecond time resolution enables us to determine the second-order ro-vibrational constant $gamma_e$ and assess new possibilities of controlling decoherence.Comment: submitted at Physical Review

Magnet Laboratory Research

Contains a report on a research project

On the characterisation of a Bragg spectrometer with X-rays from an ECR source

Narrow X-ray lines from helium-like argon emitted from a dedicated ECR source have been used to determine the response function of a Bragg crystal spectrometer equipped with large area spherically bent silicon (111) or quartz (10$\bar{1}$) crystals. The measured spectra are compared with simulated ones created by a ray-tracing code based on the expected theoretical crystal's rocking curve and the geometry of the experimental set-up.Comment: Version acceptee (NIM

Enhancing strong-field induced molecular vibration with femtosecond pulse shaping

This work investigates the utility of femtosecond pulse shaping in increasing the efficiency of Raman excitation of molecules in the strong-field interaction regime. We study experimentally and theoretically the effect of pulse shaping on the strength of non-resonant coherent anti-Stokes Raman scattering in iodine vapor at laser intensities exceeding $10^{13}$ W/cm$^2$. We show that unlike the perturbative case, shaping strong non-resonant laser pulses can increase the signal strength beyond that observed with the transform-limited excitation. Both adiabatic and non-adiabatic schemes of excitation are explored, and the differences of their potential in increasing the excitation efficiency are discussed.Comment: 10 pages, 11 figures, experimental and theoretical stud

The intercontinental schizophrenia outpatient health outcomes (IC-SOHO) study: baseline clinical and functional characteristics and antipsychotic use patterns in the North Africa and Middle Eastern (AMEA) region: original article

Objective: To describe the baseline findings of the Intercontinental Schizophrenia Outpatient Health Outcomes (IC-SOHO) study in the North Africa and Middle Eastern sub-region (AMEA-SOHO) Method: The IC-SOHO study is an ongoing prospective, three-year, non-interventional observational study of schizophrenia treatment, clinical characteristics and mental health services utilization in two North African and two Middle Eastern countries. The study population consists of non-hospitalised patients who had initiated treatment with or changed to a new antipsychotic. Results: The baseline findings of the IC-SOHO study (AMEA Subset) appear to reflect clinical practice in Turkey, Saudi Arabia, Egypt and Algeria (N=1, 398). Overall, the patients were moderately to markedly ill and either overweight (46%) or obese (8%) when they entered the study. Functionally, the majority of patients were not involved in social activities, could not care for themselves and were unemployed. Substance and alcohol dependency/abuse was not a problem in this study population. At baseline the majority of patients were treated with typical antipsychotics (oral and depot); and anticholinergics were the most commonly prescribed concomitant medication. Sexual side effects were most frequently reported among the surveyed adverse events. Overall compliance/adherence to medication was good. Conclusion: The baseline IC-SOHO data highlighted various clinical and functional characteristics and antipsychotic use patterns in a group of outpatients with schizophrenia in a naturalistic setting. Once completed, the IC-SOHO study will add further to this knowledge base. SA Psychiatry Rev. Vol.7(3) 2004: 27-3

Measuring Pancharatnam's relative phase for SO(3) evolutions using spin polarimetry

In polarimetry, a superposition of internal quantal states is exposed to a single Hamiltonian and information about the evolution of the quantal states is inferred from projection measurements on the final superposition. In this framework, we here extend the polarimetric test of Pancharatnam's relative phase for spin$-{1/2}$ proposed by Wagh and Rakhecha [Phys. Lett. A {\bf 197}, 112 (1995)] to spin $j\geq 1$ undergoing noncyclic SO(3) evolution. We demonstrate that the output intensity for higher spin values is a polynomial function of the corresponding spin$-{1/2}$ intensity. We further propose a general method to extract the noncyclic SO(3) phase and visibility by rigid translation of two $\pi /2$ spin flippers. Polarimetry on higher spin states may in practice be done with spin polarized atomic beams.Comment: New title, minor corrections, journal reference adde