Fisetin: an integrated approach to identify a strategy promoting osteogenesis

Flavonoids may modulate the bone formation process. Among flavonoids, fisetin is known to counteract tumor growth, osteoarthritis, and rheumatoid arthritis. In addition, fisetin prevents inflammation-induced bone loss. In order to evaluate its favorable use in osteogenesis, we assayed fisetin supplementation in both in vitro and in vivo models and gathered information on nanoparticle-mediated delivery of fisetin in vitro and in a microfluidic system. Real-time RT-PCR, Western blotting, and nanoparticle synthesis were performed to evaluate the effects of fisetin in vitro, in the zebrafish model, and in ex vivo samples. Our results demonstrated that fisetin at 2.5 μM concentration promotes bone formation in vitro and mineralization in the zebrafish model. In addition, we found that fisetin stimulates osteoblast maturation in cell cultures obtained from cleidocranial dysplasia patients. Remarkably, PLGA nanoparticles increased fisetin stability and, consequently, its stimulating effects on RUNX2 and its downstream gene SP7 expression. Therefore, our findings demonstrated the positive effects of fisetin on osteogenesis and suggest that patients affected by skeletal diseases, both of genetic and metabolic origins, may actually benefit from fisetin supplementation

Inclusão de alho como vermífugo, promotor de crescimento e análise da qualidade sensorial da carne do Jundiá / Inclusion of garlic as a vermifuge, growth promoter and analysis of the sensory quality of Silver Catfish meat

Alguns alimentos podem ser utilizados para a melhoria da qualidade de vida dos animais de produção, contribuindo com o bem estar e aumentando a resistência a doenças. O estudo objetivou avaliar a inclusão do alho em pó em dietas para peixes como vermífugo, promotor de crescimento e verificar sua influência na qualidade sensorial do filé. Os experimentos foram conduzidos nas instalações Instituto Federal Farroupilha – campus Júlio de Castilhos, realizados dois experimentos, primeiro testou-se a utilização do alho como vermífugo e promotor de crescimento e no segundo, como promotor de crescimento e qualidade sensorial do filé. Foram analisados três tratamentos em triplicata nos dois experimentos realizados. No experimento I, ao final do período experimental, não obtivemos resultados significativos de crescimento, mas uma redução de 97 % nos casos de verminoses. No experimento II, os animais alimentados com a dieta Controle e adicionada de 1 % de alho em pó apresentaram maior peso final, que os animais que receberam a dieta com 0,5 % alho, a redução total dos casos de verminoses. Na avaliação da qualidade da carne, o índice de aceitabilidade foi >70 % para todas as amostras testadas. E na análise sensorial, tanto as pessoas de gênero masculino quanto as do gênero feminino aprovaram a carne produzida com a inclusão do alho na ração. O alho quando adicionado à dieta foi eficaz como vermífugo alternativo para cultivo de jundiás, melhorou o crescimento dos peixes e não prejudicou a qualidade sensorial da carne

Micropropagation of Malus sieboldii hybrids resistant to apple proliferation disease

72009 CIC 1restrictedInternational coauthor/editorNatural resistance to apple proliferation (AP) disease was found in apomictic Malus sieboldii-derived genotypes which can be used as rootstocks for apple. Whereas their agronomic value is currently improved in ongoing breeding programs they are recalcitrant to propagation by standard procedures. Therefore, a micropropagation protocol was developed for in vitro establishment, multiplication and rooting of eleven interesting AP-resistant genotypes. Four different macro and micro element formulations were tested: MS, QL, WPM and DKW. Phytohormones (0.25 µM IBA, 4.44 µM BAP and 0.28 µM GA3) and vitamins (MS modified for thiamine at 2.96 µM), established for the propagation of M. × domestica, were also suitable for the propagation of M. sieboldii genotypes. The MS medium yielded in general the highest proliferation rates and the best shoot growth. A significant improvement of the growth was obtained by replacing Fe-EDTA by Fe-EDDHA as iron source. By comparing four different rooting treatments a significantly higher percentage of rooting was observed when the induction was carried out in the dark with 25 µM IBA either in liquid or agar-solidified medium. Three classes of genotypes with low, medium and high rooting efficiency were found. The acclimatisation method used yielded survival rates between 90-100% for most of the genotypesrestrictedCiccotti, A.M.; Bisognin, C.; Battocletti, I.; Salvadori, A.; Herdemertens, M.; Wallbraun, M.; Jarausch, W.Ciccotti, A.M.; Bisognin, C.; Battocletti, I.; Salvadori, A.; Herdemertens, M.; Wallbraun, M.; Jarausch, W

MICROPROPAGATION OF MALUS SIEBOLDII HYBRIDS RESISTANT TO APPLE PROLIFERATION DISEASE

Natural resistance to apple proliferation (AP) disease was found in apomictic Malus sieboldii-derived genotypes which can be used as rootstocks for apple. Whereas their agronomic value is currently improved in ongoing breeding programs they are recalcitrant to propagation by standard procedures. Therefore, a micropropagation protocol was developed for in vitro establishment, multiplication and rooting of eleven interesting AP-resistant genotypes. Four different macro and micro element formulations were tested: MS, QL, WPM and DKW. Phytohormones (0.25 μM IBA, 4.44 μM BAP and 0.28 μM GA3) and vitamins (MS modified for thiamine at 2.96 μM), established for the propagation of M. × domestica, were also suitable for the propagation of M. sieboldii genotypes. The MS medium yielded in general the highest proliferation rates and the best shoot growth. A significant improvement of the growth was obtained by replacing Fe-EDTA by Fe-EDDHA as iron source. By comparing four different rooting treatments a significantly higher percentage of rooting was observed when the induction was carried out in the dark with 25 μM IBA either in liquid or agar-solidified medium. Three classes of genotypes with low, medium and high rooting efficiency were found. The acclimatisation method used yielded survival rates between 90-100% for most of the genotype