GABA-A receptors in mPOAH simultaneously regulate sleep and body temperature in freely moving Rats

Sleep-wakefulness and body temperature are two circadian rhythmic biological phenomena. The role of GABAergic inputs in the medial preoptico-anterior hypothalamus (mPOAH) on simultaneous regulation of those phenomena was investigated in freely moving normally behaving rats. The GABA-A receptors were blocked by microinjecting picrotoxin, and the effects on electrophysiological parameters signifying sleep-wakefulness, rectal temperature and brain temperature were recorded simultaneously. The results suggest that, normally, GABA in the medial preoptic area acts through GABA-A receptor that induces sleep and prevents an excessive rise in body temperature. However, the results do not allow us to comment on the cause and effect relationship, if any, between changes in sleep-wakefulness and body temperature. The changes in brain and rectal temperatures showed a positive correlation, however, the former varied within a narrower range than that of the latter

Cardiovascular Risk Predictors High Sensitivity C-Reactive Protein and Plasminogen Activator Inhibitor-1 in Women with Lean Phenotype of Polycystic Ovarian Syndrome: A Prospective Case-Control Study

Objective Accumulating evidence suggests increased cardiovascular risk in women with polycystic ovarian syndrome (PCOS) due to a cluster of factors, such as obesity, lipid abnormalities, impaired glucose tolerance (IGT), and hypertension. Markers such as high-sensitivity C-reactive protein (hs-CRP) and plasminogen activator inhibitor-1 (PAI-1) can provide an adjunctive method for the assessment of cardiovascular risk and can indicate future coronary heart diseases in women with lean PCOS. Materials and Methods In this prospective case-control study, women clinically diagnosed with PCOS (n = 25) with normal body mass index (BMI) and age and BMI-matched healthy controls (n = 75) were enrolled. The quantitative data were expressed as mean ± standard deviation (SD). Unpaired Student's t-test was used to compare the values (PCOS vs. controls) and Pearson's correlation coefficient was used to elucidate the relationship between the variables. Results The mean level of fasting blood sugar, serum total cholesterol, low-density lipoprotein (LDL), thyroid-stimulating hormone (TSH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), hs-CRP, and PAI-1 were significantly increased in PCOS patients (p < 0.000) compared with the control patients. Of the reported cases, 54.16% had hs-CRP levels greater than 3 mg/L. When the cases were further divided into normal (n = 20) and IGT (n = 5), hs-CRP values were significantly higher in IGT group as compared with normal glucose tolerance (NGT) group. On bivariate correlation analysis, hs-CRP had significant correlations with PAI-1 (r = 0.41, p < 0.000), waist-to-hip ratio (WHR; r = 0.23, p = 0.02), fasting blood sugar (FBS; r = 0.26, p = 0.009), LDL (r = 0.20, p = 0.03), TSH (r = 0.42, p < 0.000), and LH-to-FSH ratio (r = 0.24, p = 0.01). Conclusion Women with lean phenotype of PCOS suffer from many metabolic abnormalities such as abdominal obesity, dyslipidemia, hyperandrogenemia, and insulin resistance. The findings of the study suggest that environment of ongoing low-grade inflammation due to infiltration further exacerbates the metabolic derangements and cardiovascular risk. The investigations as hs-CRP and PAI-1 will help in early identification, diagnosis, and management of cardiovascular diseases associated with lean type of PCOS. These markers can prove to be beneficial in monitoring any unfavorable changes in cardiometabolic profile of such patients

Triple plating of tibia in a complex bicondylar tibial plateau fracture

High-energy tibial plateau fracture poses a significant challenge and difficulty for orthopaedic surgeons. Fracture of tibial plateau involves major weight bearing joint and may alter knee kinematics. Anatomic reconstruction of the proximal tibial articular surfaces, restoration of the limb axis (limb alignment) and stable fixation permitting early joint motion are the goals of the treatment. In cases of complex bicondylar tibial plateau fractures, isolated lateral plating is frequently associated with varus malalignment and better results have been obtained with bilateral plating through dual incisions. However sometimes a complex type of bicondylar tibial plateau fractures is encountered in which medial plateau has a biplaner fracture in posterior coronal plane as well as sagittal plane. In such fractures it is imperative to fix the medial plateau with buttressing in both planes. One such fracture pattern of the proximal tibia managed by triple plating through dual posteromedial and anterolateral incisions is discussed in this case report with emphasis on mechanisms of this type of injury, surgical approach and management. Key words: Tibial fractures; Bone plates; Kne

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Not AvailableFeasibility of culturing pengba (Osteobrama belangeri) as a candidate species in polyculture along with other carps in net pen enclosure was studied in Takmu lake, Manipur. A net pen measuring 0.1 ha was constructed using nylon net supported by bamboo poles. The average length and weight of stocked fingerlings (@5 no. m-2) were as follows: pengba (9.4 cm, 10.6 g), Labeo catla (16.1 cm, 39.0 g), Labeo rohita (14.8 cm, 38.6 g), Cirrhinus mrigala (16.2 cm, 41.9 g), Hypophthalmichthys molitrix (14.2 cm, 30.3 g), Ctenopharyngodon idella (22.4 cm, 115.2 g) and Cyprinus carpio (14.8 cm, 55.1 g). Fishes were fed pelleted feed (23.4% crude protein) @ 5% of body weight twice-a-day for 180 days. Highest growth (in terms of weight gain % and specific growth rate) was recorded in pengba followed by C. idella, L. catla, C. mrigala, L. rohita, C. carpio and H. molitrix. The pen aquaculture system was also found to be economically viable with a benefit-cost ratio of 1.37. In conclusion, net pen aquaculture of pengba along with other carps is economically viable and technically feasible in floodplain wetlands of Manipur.ICA

Seasonal modulation of deep slow-slip and earthquakes on the Main Himalayan Thrust.

The interaction between seasonally-induced non-tectonic and tectonic deformation along the Himalayan plate boundary remains debated. Here, we propose that tectonic deformation along this plate boundary can be significantly influenced by the deformation induced by the non-tectonic hydrological loading cycles. We explore seasonal mass oscillations by continental water storage in Southeast Asia and Himalayan arc region using continuous Global Positioning System measurements and satellite data from the Gravity Recovery and Climate Experiment. We suggest that the substantially higher transient displacements above the base of the seismogenic zone indicate a role of changes in aseismic slip rate on the deep megathrust that may be controlled by seasonal hydrological loading. We invoke modulation of aseismic slip on the megathrust down-dip of the seismogenic zone due to a fault resonance process induced by the seasonal stress changes. This process modulates mid-crustal ramp associated micro-seismicity and influences the timing of Central Himalayan earthquakes

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Not AvailableTo establish normal values of blood biochemical indices for different indigenous local donkey population available in various agro-climatic zones, blood samples were collected from 233 adult and apparently healthy donkeys. The samples were analysed for metabolites (albumin, total serum protein, direct bilirubin, total bilirubin, cholesterol, HDL cholesterol, urea, uric acid, triglyceride, creatinine) and minerals (calcium, phosphorus) to evaluate significant difference within and between populations. Confidence limit of each biochemical indices showed a close range as compared to their actual range observed under varied geographic areas. All the metabolites and minerals showed significant variations in their levels within and between donkey populations which could possibly be due to the differences in the nutritional status of donkeys, their managemental aspects and biochemical metabolism. In agro-climatic zone 1 (Spiti and Leh areas), having low vegetation cover with poor nutritious grasses for a limited period, levels of most of the biochemical profiles in donkey populations belonging to these areas were significantly lower than those in other zones (VI, IX, XII, XIV). This study indicated that normal biochemical values of different indices for a particular population should not be used as such for disease prognosis, diagnosis and therapeutic monitoring of other donkey population belonging to other agro-climatic zone having different nutritional and managemental practicesNot Availabl

Descriptive epidemiology of equine influenza in India (2008-2009): temporal and spatial trends

Equine influenza is a contagious viral disease that affects all members of the family Equidae, i.e. horses, donkeys and mules. The authors describe the pattern of equine influenza outbreaks in a number of states of India from July 2008 to June 2009. The disease was first reported in June 2008 in Katra (Jammu and Kashmir) and spread to ten other states within a year. All outbreaks of equine influenza in the various states were confirmed by laboratory investigations (virus isolation and/or serological confirmation based on haemagglutination inhibition [HI] assays of paired samples) before declaring them as equine influenza virus-affected state(s). The virus (H3N8) was reported from various locations in the country including Katra, Mysore (Karnataka), Ahmedabad (Gujarat), Gopeshwar and Uttarkashi (Uttarakhand) and was isolated in 9- to 11-day-old embryonated chicken eggs. The virus was confirmed as H3N8 by HI assays with standard serum and amplification of full-length haemagglutinin and neuraminidase genes by reverse transcriptase-polymerase chain reaction. Serum samples (n = 4 740) of equines from 13 states in India screened by HI revealed 1 074 (22.65%) samples as being positive for antibodies to equine influenza virus (H3N8)