Application of probiotics and prebiotics for promoting growth of Tiger shrimp (Penaeus monodon): an approach to eco-friendly shrimp aquaculture

The current study has been conducted to evaluate the growth performance of shrimp (Penaeus monodon) by applying eco-friendly culture mechanism like prebiotics and probiotics. The experiment was carried out for 95 days in different shrimp farms at coastal district of Bagerhat, Bangladesh. Three different treatments viz., probiotic treated as T1, prebiotics treated as T2 and both probiotics and prebiotics as T3 with a control group were designed to conduct the experiment. The size of the experimental ponds was five acre and the stocking density was 4/m2 in each treatment. CP NASA shrimp feed (32% protein) was given thrice in a day during the study period. After 95 days of culture period, the maximum weight gain was observed at T3 (33.78±0.18 g) whereas the minimum weight gain was observed at control group (25.69±0.10 g). The survival rate was the highest in T3 (89.01%) followed by T2 (75.51%) and T1 (53.44%) and the lowest rate was observed in control group (50.88%). Overall production was higher in T3 (833.78 kg ha-1) compared to T2 (553.40 kg ha-1), T1 (447.84 kg ha-1) and Control group (310.57 kg ha-1). pH value was found to maximum in T3 (7.71±0.08) and it was minimum in T1 (7.41±0.10). In addition, the maximum TAN value was found to be 2.22±0.19 mg L-1 in C pond and it was minimum in T3 (0.32±0.06 mg L-1). Therefore, it could be concluded that combine application of probiotics and prebiotics might be the reliable media to enhance production of shrimp by maintaining eco-friendly environment in aquaculture. Int. J. Agril. Res. Innov. Tech. 10(2): 15-20, December 202

Application of probiotics and prebiotics for promoting growth of Tiger shrimp (Penaeus monodon): an approach to eco-friendly shrimp aquaculture

The current study has been conducted to evaluate the growth performance of shrimp (Penaeus monodon) by applying eco-friendly culture mechanism like prebiotics and probiotics. The experiment was carried out for 95 days in different shrimp farms at coastal district of Bagerhat, Bangladesh. Three different treatments viz., probiotic treated as T1, prebiotics treated as T2 and both probiotics and prebiotics as T3 with a control group were designed to conduct the experiment. The size of the experimental ponds was five acre and the stocking density was 4/m2 in each treatment. CP NASA shrimp feed (32% protein) was given thrice in a day during the study period. After 95 days of culture period, the maximum weight gain was observed at T3 (33.78±0.18 g) whereas the minimum weight gain was observed at control group (25.69±0.10 g). The survival rate was the highest in T3 (89.01%) followed by T2 (75.51%) and T1 (53.44%) and the lowest rate was observed in control group (50.88%). Overall production was higher in T3 (833.78 kg ha-1) compared to T2 (553.40 kg ha-1), T1 (447.84 kg ha-1) and Control group (310.57 kg ha-1). pH value was found to maximum in T3 (7.71±0.08) and it was minimum in T1 (7.41±0.10). In addition, the maximum TAN value was found to be 2.22±0.19 mg L-1 in C pond and it was minimum in T3 (0.32±0.06 mg L-1). Therefore, it could be concluded that combine application of probiotics and prebiotics might be the reliable media to enhance production of shrimp by maintaining eco-friendly environment in aquaculture.&#x0D; Int. J. Agril. Res. Innov. Tech. 10(2): 15-20, December 2020</jats:p

Cytogenetics, genome size and anther anatomy in Bulbostylis

Fil: López, María Gabriela. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: López, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Fernández, Aveliano. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.Fil: Fernández Aveliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Fil: Galdeano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.Cyperaceae display diverse and unusual cytogenetical characteristics, but are still sparsely documented and poorly understood in this respect. Six new chromosome counts are presented for Bulbostylis. Absolute DNA content was close to 1 pg in all species and was independent from chromosome number. Despite variations in chromosome number from 2n = 30 to 2n = 102, DNA content only varied from 0.932 pg to 1.198 pg. The studies presented in this work showed that species possess holokinetic chromosomes. The size of stomata and pollen grains did not vary. These results would confirm that the chromosome evolution of these species was via agmatoploidy and symploidy rather than by polyploidy. Abnormalities during meiotic spindle formation in microsporogenesis followed by simultaneous cytokinesis resulted in four microspores, of which only one was functional, resulting in a pseudomonad maturing into pollen grains with the usual vegetative and generative nuclei in addition to three degenerated microspore nuclei

Antiviral activity of Cynodon dactylon on white spot syndrome virus (WSSV)-infected shrimp: an attempt to mitigate risk in shrimp farming

White spot syndrome virus (WSSV) is the most contagious pathogen causing huge economic losses in the shrimp industry worldwide. Several medicinal plants are known to have antiviral activity through the inhibition of viral diseases in fish and shellfish. Hence, there is a need to investigate the ability of natural remedies like plant sources to mitigate this devastating disease in crustaceans. This study was carried out to test the antiviral activity of ethanolic extract of Cynodon dactylon on WSSV in tiger shrimp Penaeus monodon juveniles with an average weight of 13.541 ± 2.927 g. Different doses of the extract (75, 100, and 150 mg/kg) were administrated in vivo through intramuscular injection. The antiviral activity was determined by observing survival rates, and WSSV infection was confirmed at the end of the experiment through polymerase chain reaction (PCR) identification. Before the in vivo experiment, presence of antiviral compounds ((+)-catechin, vanillic acid, syringic acid, (−)-epicatechin, p-coumaric acid, and quercetin bioactive compounds) in C. dactylon was confirmed through high-performance liquid chromatography with diode-array detection (HPLC-DAD) analysis. The results obtained in this study show that a dose of 100 to 150 mg/kg body weight ethanolic extract of C. dactylon prevented the infection of WSSV marked by 100% survival and absence of WSSV-specific band using nested diagnostic PCR, thus demonstrating the suitability of ethanolic extract of C. dactylon as a possible prophylactic for the prevention of WSSV infections in shrimp culture.</p

Application of zymetin and super PS probiotics in hatchery, nursery, and grow-out phases of Macrobrachium rosenbergii and their impact on culture environment, production, and economics

This study evaluated the effect of two commercial probiotics, zymetin, and super PS, on the culture environment, production, and economics of Macrobrachium rosenbergii aquaculture. The experiment was conducted using three treatments, including T₁, probiotics applied in all culture phases; T₂, during grow-out; and a control (C). Prawns were fed commercial pelleted diets. Earthen ponds were used at a stocking density of two juveniles/m². Water concentrations of non-ionized ammonia and nitrite-nitrogen were significantly lower (p < 0.05) in T₁ than in T₂ and C. Probiotic application significantly (p < 0.05) reduced phosphate content and balanced soil pH. Growth performance, condition factor, survival, protein utilization, and production were significantly higher in T₁ followed by T₂ compared to C by the end of grow-out. Application of zymetin and super PS in all culture phases significantly (p < 0.05) reduced the feed conversion ratio in T₁compared to T₂ and C. Positive allometric growth was observed in T₁, whereas growth was isometric in C and T₂. Probiotic treatment during all culture phases achieved the highest net returns to land, family labor, and management. Application of probiotics during the entire culture cycle could be the best practice for improving production and economics in M. rosenbergii aquaculture