Applicazione di un veloce metodo di estrazione del DNA per l’identificazione di prodotti ittici marini.

L'identificazione dei prodotti ittici è uno dei temi chiave in materia di sicurezza alimentare. L’errata etichettatura dei prodotti alimentari e la sostituzione di alcuni ingredienti rappresentano questioni emergenti in termini di qualità e sicurezza alimentare e nutrizionale. L'autenticazione e la tracciabilità dei prodotti alimentari, gli studi di tassonomia e di genetica di popolazione, così come l'analisi delle abitudini alimentari degli animali e la selezione delle prede, si basano su analisi genetiche tra cui la metodica molecolare del DNA barcoding, che consiste nell’amplificazione e nel sequenziamento di una specifica regione del gene mitocondriale chiamata COI. Questa tecnica biomolecolare è utilizzata per fronteggiare la richiesta di determinazione specifica e/o la reale provenienza dei prodotti commercializzati, nonché per smascherare errori di etichettatura e sostituzioni fraudolente, difficile da rilevare soprattutto nei prodotti ittici trasformati. Sul mercato sono disponibili differenti kit per l'estrazione del DNA da campioni freschi e conservati; l’impiego dei kit, aumenta drasticamente il costo dei progetti di caratterizzazione e di genotipizzazione dei campioni da analizzare. In questo scenario è stato messo a punto un metodo veloce di estrazione del DNA. Esso non prevede nessuna fase di purificazione per i prodotti ittici freschi e trasformati e si presta a qualsiasi analisi che preveda l’utilizzo della tecnica PCR. Il protocollo consente l'amplificazione efficiente del DNA da qualsiasi scarto industriale proveniente dalla lavorazione del pesce, indipendentemente dal metodo di conservazione del campione. L’applicazione di questo metodo di estrazione del DNA, combinato al successo e alla robustezza della amplificazione PCR (secondo protocollo barcode) ha permesso di ottenere, in tempi brevissimi e con costi minimi, il sequenziamento del DNA

OpenCoesione experience: citizen is the best communicator!

Communication plays an essential role in scientific projects, especially since it is possible to use social media and web platforms, to reach a wide audience. OpenCoesione is the “open-government” iniziative on cohesion polizie in Italy, a web plat form with navigable data on public programmed resources and expenses, that allows to evaluate how there sources are used. Any citizen can enrich this platform, analysing public projects and documentino the current state with open data. «A Scuola di OpenCoesione» is an innovative experimental project of school teaching of open government strategy of openness and transparency on development policies. The goal of A Scuola di Open Coesione is to involve and sensitize students and teachers to the use of contents and tools to learn and monitor the projects of cohesion policies, to build interdisciplinary educational programs starting from the projects financed on their respective territories and enriching, through crowd sourcing, the information base of OpenCoesione adding information and data that are not currently availableas, for example, geolocation,multimedia materials, the characteristics of the project, the physical performance of the works

DNA Barcoding as a tool for Zoological Taxonomy: Identification of bony fish in the Mediterranean Sea

The description of all the species present in nature is a vast task to be fulfilled by using the classical approach of morphological description of the organisms. In recent years, the traditional taxonomy, based primarily on identification keys of species, has shown a number of limitations in the use of the distinctive features in many animal taxa and inconsistencies with the genetic data. Furthermore, the increasing need to get a true estimate of biodiversity has led Zoological Taxonomy to seek new approaches and methodologies to support the traditional methods. The classification procedure has added modern criteriasuch as the evolutionary relationships and the genetic, biochemical and morphological characteristics of the organisms.Until now the Linnean binomial was the only abbreviated code associated with the description of the morphology of a species. The new technologies aim to achieve a short nucleotide sequence of the DNA to be used as an unique and solely label for a particular species, a specific genetic barcode. For both morphological and genetic approaches, skills and experience are required. Taxonomy is one of zoological disciplines that has been benefited from the achievements reached by modern molecular biotechnology. Using a molecular approach it is possible to identify cryptic species, to establish a family relationship between species and their membership of taxonomic categories or to reconstruct the evolutionary history of a taxon

The absolute calibration strategy of the ASTRI SST-2M telescope proposed for the Cherenkov Telescope Array and its external ground-based illumination system

ASTRI is the end-to-end prototype for the CTA small-size class of telescopes in a dual-mirror configuration (SST-2M) proposed by the Italian National Institute of Astrophysics (INAF) in the framework of the Cherenkov Telescope Array. ASTRI SST-2M has been installed at the Serra La Nave Astrophysical Observatory on Mount Etna (Sicily) and its Performance Verification Phase will start in autumn 2016. For the relative pixel calibration and gain monitoring, the ASTRI SST-2M camera is equipped with an internal illumination device, while an external, portable, illumination system, placed at a few km distance from the telescope, will be used for the absolute end-to-end calibration of the telescope spectral response. Moreover analysis of signals induced in the camera pixels by the night sky background (diffuse emission and reference stars) will be used to monitor the long term evolution of the telescope calibration. We present an overview of the ASTRI SST-2M absolute calibration strategy and the external illuminating device that will be used for its spectral calibratio

Exploiting videos for science communication: the edulab experience

Nowadays, science communication and popularisation are part of a scientist’s everiday life because because divulgation disseminates scientific information to the general pubblic, helping himto under stand specific scientific subject and the advancements of research, as well as inspiring critical reflection, awareness and environmentally responsible behaviours. A team of scientists, researches and technicians of the IAS-CNR of Capo Granitola welcomed this challenge, founding the EDULab group. Its core activities are addressed in science divulgation and experimentation of innovative approaches using emerging digital technologies. Among a variety of science comunication products, videos have proved to be one of the best ways to litterally «immerse» the audience in scientific research and make the experience fun, in particular for non scientic audience and young people

How much biodiversity is concealed in the word ‘biodiversity’?

Non peer reviewe

Quando la scienza brancola nel buio: accordo di collaborazione tra l’Istituto dei Ciechi “Opere riunite I. Florio – F. ed A. Salamone” di Palermo e il CNR IAS di Capo Granitola

La convenzione oggetto del presente rapporto nasce nel contesto delle complesse attività di EduLab, gruppo di divulgazione scientifica che,seppur già attivo dal2008, nasce ufficialmente nel 2015e mette insieme ricercatori di varie discipline che condividono la passione di divulgare la scienza e i risultati da essa prodotti nei propri ambiti di competenza.La comunicazione scientifica, molto sentita e ben strutturata all’interno dell’IAS (ex. IAMC), ha portato a numerosi risultati e ad un numero ragguardevole di progetti e programmi, ed è un’attività ormai talmente ben consolidata da diventare organica all’istituto e alle sue strategie. I prodotti sviluppati sono tanti e anche i successi ottenuti, quali premi sulle attività di divulgazione, la partecipazione ad Expo 2015 ed a numerose edizioni in ambito di manifestazioni nazionali quali: Festival della Scienza, Esperienza insegna, Notte della scienza e Notte dei ricercatori, Illuminiamo la scienza. Grazie ad un curriculum unico e complesso, il gruppo è stato il driver della nascita dell’Osservatorio della Biodiversità della Regione Sicilia, oggi riferimento regionale per la didattica e la divulgazione scientifica sul tema della biodiversità, visitato ad oggi da più di 500 classi di studenti siciliani. Le ricadute culturali, di visibilità, nonché di immagine per l’istituto sono tantissime

“How a good video can remove ‘barriers’ for a more inclusive science communication”

There is a burgeoning body of evidence suggesting that technology can enhance learning, and multiple studies have shown that videos represent a very effective tool in science engagement and education. In fact, video media can transform the complexities of science and nature into something more tangible and tractable (Dabylchuk et al., 2018). Documentaries can hence be powerful tools for learning, to raise awareness for important topics, and adding a scientific approach makes it possible to deepen knowledge about the world. According to Dale’s cone of experience (Fig. 1), people memorize 10% of what they read, 20% of what they hear, 30% of what they see and 50% of what they hear and see (Wiman and Mierhenry, 1969). These statistics seems to convey a very clear message: blind or visually impaired people are penalised in the learning compared to sighted ones. However, our experience in the area of inclusive science communication has led us to wonder: are we sure that sight is a fundamental means for learning? Or is it possible that sight is a predominant sense over others, and therefore it can even mislead or limit learning? To answer this question, we held an ad-hoc educational laboratory with the users of the “IstitutodeiCiechiOpereRiunite I. Florio – F. ed A. Salamone” of Palermo, with which we have established a convention on 29/01/2019. Specifically, we involved 20 users with different degrees of visual impairment, in the projection of three science videos, selected from those realised by the EDUlab divulgation group over the years. At the end of the video projection, users were asked to express their opinions and their personal interpretations of scientific issues, allowing us to understand what e how many information have been received and, above all, if sighted usersare really advantaged compared to the visually impaired ones

“Beaks and berries”: zoological laboratory (II) of the formative proposal of inclusive scientific divulgation

Searching a topic that help us about the implementation of an inclusive divulgation, aimed also at sensory disabilitiesusers, we have chosen birds as a key to connect different scientific issues (pollution, environmental changes, exploitation of resources) and create new knowledge in a heterogeneous auditorium, through the suggestions that the workshop lessons can infuse on the users, whether they are disabled or not. All this to bring them closer to thescientific contents to which they do not have easy access. In particular, the project aims to develop, deepen and consolidate, through communication and information, issues concerning the concept of biodiversity, in order to sensitize users to safeguard the species and habitats of our territory and make them as the main actors of scientific dissemination.Indeed, weestabilisheda convention between the “Istituto dei Ciechi Opere riunite I. Florio – F. ed A. Salamone” of Palermo and the “Consiglio Nazionale delle Ricerche - Istituto per lo studio degli impatti Antropici e Sostenibilità in ambiente marino” of Capo Granitola (TP). The main role of this convention is to bring disable people closer to these scientific subjects and make the contents of our laboratories as inclusive as possible.The project "The world of birds", developed from January to April 2019 is divided into three main themes, the second of which is the focus of this report: The bird alimentation