Empirical Analysis of Subsidy Industrial Policy’s Effect on Export Innovation in the Chinese Manufacturing

The relatively low export innovation capacity is not conductive to the steady transformation and upgrading of China’s manufacturing industry, and it is necessary to implement suitable policies to enhance export innovation capacity. This study empirically analyzes the data of 827,471 manufacturing enterprises from 2000 till 2013 to investigate the impact of subsidy policy on export innovation. The overall results show that China’s subsidy policy has a significant crowding-out effect on export innovation, and subsidy for relatively small enterprises is more conducive to promoting export innovation; however, enterprises’ independent investment does not own much impact on export innovation. The econometric results made from perspectives of the sub-region and the industry reveal that subsidy policy is not conducive to export innovation, independent investment is not beneficial to export innovation, and export innovation ability is positively correlated with enterprise scale, but the influence coefficient shows obvious differences

Research Progress on Factors Affecting Oil-Absorption Performance of Cement-Based Materials

With the wide application of petroleum resources, oil substances have polluted the environment in every link from crude oil extraction to utilization. Cement-based materials are the main materials in civil engineering, and the study of their adsorption capacity for oil pollutants can expand the scope of functional engineering applications of cement-based materials. Based on the research status of the oil-wet mechanism of different kinds of oil-absorbing materials, this paper lists the types of conventional oil-absorbing materials and introduces their application in cement-based materials while outlining the influence of different oil-absorbing materials on the oil-absorbing properties of cement-based composites. The analysis found that 10% Acronal S400F emulsion can reduce the water absorption rate of cement stone by 75% and enhance the oil-absorption rate by 62%. Adding 5% polyethylene glycol can increase the oil–water relative permeability of cement stone to 1.2. The oil-adsorption process is described by kinetic and thermodynamic equations. Two isotherm adsorption models and three adsorption kinetic models are explained, and oil-absorbing materials and adsorption models are matched. The effects of specific surface area, porosity, pore interface, material outer surface, oil-absorption strain, and pore network on the oil-absorption performance of materials are reviewed. It was found that the porosity has the greatest influence on the oil-absorbing performance. When the porosity of the oil-absorbing material increases from 72% to 91%, the oil absorption can increase to 236%. In this paper, by analyzing the research progress of factors affecting oil-absorption performance, ideas for multi-angle design of functional cement-based oil-absorbing materials can be obtained

Migration and distribution of complex fracture proppant in shale reservoir volume fracturing

In this paper, a large-scale experimental system was established to identify the migration and distribution laws of complex fracture proppant in shale reservoir volume fracturing. With this system, the effects of secondary fracture angle, fluid displacement, proppant concentration and size, fracturing fluid viscosity and other factors on the migration and distribution of proppant were tested, and the migration and distribution of proppant in primary/secondary fractures were analyzed. The following results were obtained. First, the fluid flow pattern in fractures transforms gradually from laminar flow into turbulent flow with the increase of fracture supporting height. Second, the migration modes of proppant in fractures mainly include suspended migration and gliding migration. Third, the distribution form of proppant in primary fractures before branching is related to secondary fracture angle, fluid displacement and proppant concentration and size, among which the fluid displacement is the most important factor. Fourth, the mass ratio of proppant in primary fractures after branching is proportional to the secondary fracture angle, fluid displacement, fracturing fluid viscosity and proppant concentration and size, and is inversely proportional to the flow ratio between secondary fractures and primary fractures. Fifth, the mass ratio of proppant in secondary fractures after branching is proportional to fluid displacement, fracturing fluid viscosity and flow ratio between secondary fractures and primary fractures, and is inversely proportional to secondary fracture angle and proppant concentration and size. Sixth, the angle at the leading edge of proppant bank in the primary fractures after branching is proportional to the proppant concentration and size and the flow ratio between secondary fractures and primary fractures, but is inversely proportional to secondary fracture angle, fluid displacement and fracturing fluid viscosity. Seventh, the angle at the leading edge of proppant bank in the secondary fractures after branching is proportional to the secondary fracture angle and the proppant concentration and size, but is inversely proportional to the fluid displacement, fracturing fluid viscosity and flow ratio between secondary fractures and primary fractures. In conclusion, the research results can provide a theoretical support for proppant optimization and program design of shale reservoir volume fracturing. Keywords: Shale, Reservoir, Volume fracturing, Complex fracture, Proppant, Migration and distribution, Experimental syste

Transcriptome Sequencing to Identify Important Genes and lncRNAs Regulating Abdominal Fat Deposition in Ducks

Abdominal fat deposition is an important trait in meat-producing ducks. F2 generations of 304 Cherry Valley and Runzhou Crested White ducks were studied to identify genes and lncRNAs affecting abdominal fat deposition. RNA sequencing was used to study abdominal fat tissue of four ducks each with high or low abdominal fat rates. In all, 336 upregulated and 297 downregulated mRNAs, and 95 upregulated and 119 downregulated lncRNAs were identified. Target gene prediction of differentially expressed lncRNAs identified 602 genes that were further subjected to Gene Ontology and KEGG pathway analysis. The target genes were enriched in pathways associated with fat synthesis and metabolism and participated in biological processes, including Linoleic acid metabolism, lipid storage, and fat cell differentiation, indicating that these lncRNAs play an important role in abdominal fat deposition. PPAPA, FOXO3, FASN, PNPLA2, FKBP5, TCF7L2, BMP2, FGF2, LIFR, ZBTB16, SIRT, GYG2, NCOR1, and NR3C1 were involved in the regulation of abdominal fat deposition. PNPLA2, TCF7L2, FGF2, LIFR, BMP2, FKBP5, GYG2, and ZBTB16 were regulated by the lncRNAs TCONS_00038080, TCONS_0033547, TCONS_00066773, XR_001190174.3, XR_003492471.1, XR_003493494.1, XR_001192142.3, XR_002405656.2, XR_002401822.2, XR_003497063.1, and so on. This study lays foundations for exploring molecular mechanisms underlying the regulation of abdominal fat deposition in ducks and provides a theoretical basis for breeding high-quality meat-producing ducks

Metabolomics and Proteomics Characterizing Hepatic Reactions to Dietary Linseed Oil in Duck

The imbalance in polyunsaturated fatty acid (PUFA) composition in human food is ubiquitous and closely related to obesity and cardiovascular diseases. The development of n-3 PUFA-enriched poultry products is of great significance for optimizing fatty acid composition. This study aimed to improve our understanding of the effects of dietary linseed oil on hepatic metabolism using untargeted metabolomics and 4D label-free proteome analysis. A total of 91 metabolites and 63 proteins showed differences in abundance in duck livers between the high linseed oil and control groups. Pathway analysis revealed that the biosynthesis of unsaturated fatty acids, linoleic acid, glycerophospholipid, and pyrimidine metabolisms were significantly enriched in ducks fed with linseed oil. Meanwhile, dietary linseed oil changed liver fatty acid composition, which was reflected in the increase in the abundance of downstream metabolites, such as α-linolenic acid (ALA; 18:3n-3) as a substrate, including n-3 PUFA and its related glycerophospholipids, and a decrease in downstream n-6 PUFA synthesis using linoleic acid (LA; 18:2n-6) as a substrate. Moreover, the anabolism of PUFA in duck livers showed substrate-dependent effects, and the expression of related proteins in the process of fatty acid anabolism, such as FADS2, LPIN2, and PLA2G4A, were significantly regulated by linseed oil. Collectively, our work highlights the ALA substrate dependence during n-3 PUFA synthesis in duck livers. The present study expands our knowledge of the process products of PUFA metabolism and provides some potential biomarkers for liver health

Effects of linseed oil supplementation duration on fatty acid profile and fatty acid metabolism-related genes in the muscles of Chinese crested white ducks

ABSTRACT: Meat rich in polyunsaturated fatty acids is considered beneficial to health. Supplementing the diet with linseed oil promotes the deposition of polyunsaturated fatty acids (PUFAs) in poultry, a conclusion that has been confirmed multiple times in chicken meat. However, fewer studies have focused on the effects of dietary fatty acids on duck meat. Therefore, this study aims to evaluate the effects of the feeding time of a linseed oil diet on duck meat performance and gene expression, including meat quality performance, plasma biochemical indicators, fatty acid profile, and gene expression. For this study, we selected 168 Chinese crested ducks at 28 days old and divided them into three groups, with 56 birds in each group. The linseed oil content in the different treatment groups was as follows: the control group (0% flaxseed oil), the 14d group (2% linseed oil), and the 28d group (2% linseed oil). Ducks in the two experimental groups were fed a linseed oil diet for 28 and 14 days at 28 and 42 days of age, respectively. The results showed that linseed oil had no negative effect on duck performance (slaughter rate, breast muscle weight, and leg muscle weight) or meat quality performance (pH, meat color, drip loss, and shear force) (P > 0.05). The addition of linseed oil in the diet increased plasma total cholesterol and high-density lipoprotein cholesterol levels (P < 0.05), while decreasing triglyceride content (P < 0.05). Furthermore, the supplementation of linseed oil for four weeks affected the composition of muscle fatty acids. Specifically, levels of α-linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid were increased (P < 0.05), while eicosatetraenoic acid content was negatively correlated with flaxseed oil intake (P < 0.05). qRT-PCR analysis further revealed that the expression of FATP1, FABP5, and ELOVL5 genes in the breast muscle, as well as FABP3 and FADS2 genes in the thigh muscle, increased after four weeks of linseed oil supplementation (P < 0.05). However, after two weeks of feeding, CPT1A gene expression inhibited fatty acid deposition, suggesting an increase in fatty acid oxidation (P < 0.05). Overall, the four-week feeding time may be a key factor in promoting the deposition of n-3 PUFAs in duck meat. However, the limitation of this study is that it remains unknown whether longer supplementation time will continue to affect the deposition of n-3 PUFAs. Further experiments are needed to explain how prolonged feeding of linseed oil will affect the meat quality traits and fatty acid profile of duck meat