Impact of Bivalirudin on Ischemia/Reperfusion Injury in Patients with Reperfused STEMI Assessed by Cardiac Magnetic Resonance

Thrombin is an important ischemia/reperfusion injury (IRI) mediator in patients with ST-elevation myocardial infarction (STEMI). This study examines the use of bivalirudin, a direct thrombin inhibitor, in reducing IRI in STEMI patients. STEMI patients (n = 21) were treated with bivalirudin and compared to 21 patients treated with unfractionated heparin (UFH) from the EARLY Assessment of Myocardial Tissue Characteristics by CMR in STEMI (EARLY-MYO-CMR) registry (NCT03768453). Infarct size (IS) and left ventricular ejection fraction (LVEF) were comparable between the two groups at follow up. During the first cardiac magnetic resonance (CMR) scan within the first week after percutaneous coronary intervention (PCI), all patients in both the bivalirudin and UFH groups exhibited myocardial edema. However, the myocardium edema volume was significantly less in the bivalirudin group (p p p < 0.05). These findings were corroborated by T2 and T1 mapping results. The study concluded that the use of bivalirudin for anticoagulation is associated with attenuated IRI in STEMI patients who receive primary PCI

Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI

Conotoxins (CTXs) are a variety of mixed polypeptide toxins, among which α-conotoxin MI (CTX-MI) is the most toxic. Serious toxic symptoms, a lack of counteracting drugs, and cumbersome detection processes have made CTX-MI a hidden danger for humans. One of the obstacles to resolving this problem is the absence of specific recognition elements. Aptamers have shown great advantages in the fields of molecule detection, drug development, etc. In this study, we screened and characterized aptamers for CTX-MI through a programmed process. MBMI-01c, the isolated aptamer, showed great affinity, with an affinity constant (KD) of 0.524 μM, and it formed an antiparallel G-quadruplet (GQ) structure for the specific recognition of CTX-MI. Additionally, an aptasensor based on the biolayer interferometry (BLI) platform was developed and displayed high precision, specificity, and repeatability with a limit of detection (LOD) of 0.26 μM. This aptasensor provides a potential tool for the rapid detection of CTX-MI in 10 min. The aptamer can be further developed for the enrichment, detoxification, and biological studies of CTX-MI. Additionally, the programmed process is applicable to screening and characterizing aptamers for other CTXs

In situ synthesis of large-area single sub-10 nm nanoparticle arrays by polymer pen lithography

In order to take advantage of the unique properties of nanoparticles in integrated devices, it is desirable to position monodispersed nanoparticles on substrates with controlled placement. Herein, we utilize small molecules such as ethylene glycol (EG) or glycerol to facilitate the delivery of nanoparticle precursors to the substrates in the polymer pen lithography (PPL) process. Subsequently, large-area ordered single nanoparticle arrays, including sub-10 nm Ag nanoparticle, 30 nm Au nanoparticle and 80 nm Fe2O3 nanoparticle arrays have been synthesized in situ with controllable sizes and pitches

Hybridization of Metal Nanoparticles with Metal–Organic Frameworks Using Protein as Amphiphilic Stabilizer

Here, a facile strategy is reported to efficiently hybridize metal nanoparticles (MNPs) with typical metal–organic frameworks (MOFs) of ZIF-8 (zeolitic imidazolate framework-8), which employs bovine serum albumin (BSA, a serum albumin protein derived from cows) as the amphiphilic stabilizer to increase the affinity of MNP toward MOFs. For instance, the as-synthesized PdNPs/ZIF-8 composites with diameter from 100 to 200 nm always maintain well-defined crystalline structure, and the PdNPs with small size of ∼2 nm are well-dispersed in the crystal of MOFs without serious aggregations due to the BSA stabilizer. In Suzuki cross-coupling reactions of aryl halide, the PdNPs/ZIF-8 as catalysts have exhibited high activity and satisfied reusability owing to the use of BSA stabilizer as well as the fixing of MOFs matrixes. In addition, the strategy also can be extended to synthesize other kinds of MNPs/MOFs hybrid composites with tunable particle size, which brings more opportunity for functional MOFs hybrid materials

Rewritable multilevel memory performance of a tetraazatetracene donor-acceptor derivative with good endurance

A new tetraazatetracene derivative, 2,3-[4,4′-bis(N,N-diphenylamino)benzyl]-5,12-bis[(triisopropylsilyl)ethynyl]-1,4,6,11-tetraazatetracene (TPAs-BTTT), displays rewritable multilevel memory behavior, which is probably induced by multielectron intramolecular charge transfer (CT)

Effects of initial mist conditions on simulation accuracy of humidity distribution in an environmental chamber

This paper firstly presents experimental study of humidity distribution in an environmental chamber at different inlet temperatures and humidity. Experiment results show that, under fixed inlet temperature condition, the uniformity of the relative humidity (RH) in the chamber is better when the inlet RH is higher. It is mainly because of the coupled heat and mass transfer occurred between water droplets and air. In practice, direct measurement of humidity distribution in a chamber is infeasible and CFD simulation is a possible way to predict the humidity distribution. However, the use of CFD tools for predicting humidity field is rarely reported. Boundary and initial mist conditions are critical to the accuracy and computation efficiency of CFD simulation. This study then presents a CFD simulation method of the humidity distribution inside chamber using FLUENT based on some assumptions and simplification. Moreover, droplet sizes were measured by laser diffraction analyzer. The simplified droplet size distribution is used as initial condition for CFD simulation. Experiment results and CFD simulation results are compared and it can be concluded that the CFD simulation method can achieve satisfactory accuracy with reasonable computation load

Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway

Over-expression of aspartyl (asparagynal)-β-hydroxylase (ASPH) contributes to hepatocellular carcinoma (HCC) invasiveness, but the role of ASPH hydroxylase activity in this process remains to be defined. As such, the current study investigated the role of ASPH hydroxylase activity in downstream signalling of HCC tumorgenesis and, specifically, metastasis development. Over-expression of wild-type ASPH, but not a hydroxylase mutant, promoted HCC cell migration in vitro, as well as intrahepatic and distant metastases in vivo. The enhanced migration and epithelial to mesenchymal transition (EMT) activation was notably absent in response to hydroxylase activity blockade. Vimentin, a regulator of EMT, interacted with ASPH and likely mediated the effect of ASPH hydroxylase activity with cell migration. The enhanced hydroxylase activity in tumor tissues predicted worse prognoses of HCC patients. Collectively, the hydroxylase activity of ASPH affected HCC metastasis through interacting with vimentin and regulating EMT. As such, ASPH might be a promising therapeutic target of HCC. Keywords: ASPH, Hydroxylase, Hepatocellular carcinoma, Metastasis, Epithelial-mesenchymal transition, Vimenti

Beclin 2 Functions in Autophagy, Degradation of G Protein-Coupled Receptors, and Metabolism

The molecular mechanism of autophagy and its relationship to other lysosomal degradation pathways remain incompletely understood. Here, we identified a previously uncharacterized mammalian-specific protein, Beclin 2, which, like Beclin 1, functions in autophagy and interacts with class III PI3K complex components and Bcl-2. However, Beclin 2, but not Beclin 1, functions in an additional&nbsp;lysosomal degradation pathway. Beclin 2 is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (GPCRs) through its interaction with GASP1. Beclin 2 homozygous knockout mice have decreased embryonic viability, and heterozygous knockout mice have defective autophagy, increased levels of brain cannabinoid 1 receptor, elevated food intake, and obesity and insulin resistance. Our findings identify Beclin 2 as a converging regulator of autophagy and GPCR turnover and highlight the functional and mechanistic diversity of Beclin family members&nbsp;in autophagy, endolysosomal trafficking, and metabolism